anterograde labeling
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Cells ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 2427 ◽  
Author(s):  
Guixin Zhang ◽  
William Rodemer ◽  
Isabelle Sinitsa ◽  
Jianli Hu ◽  
Michael E. Selzer

Many studies of axon regeneration in the lamprey focus on 18 pairs of large identified reticulospinal (RS) neurons, whose regenerative abilities have been individually quantified. Their axons retract during the first 2 weeks after transection (TX), and many grow back to the site of injury by 4 weeks. However, locomotor movements begin before 4 weeks and the lesion is invaded by axons as early as 2 weeks post-TX. The origins of these early regenerating axons are unknown. Their identification could be facilitated by studies in central nervous system (CNS) wholemounts, particularly if spatial resolution and examination by confocal microscopy were not limited by light scattering. We have used benzyl alcohol/benzyl benzoate (BABB) clearing to enhance the resolution of neuronal perikarya and regenerated axons by confocal microscopy in lamprey CNS wholemounts, and to assess axon regeneration by retrograde and anterograde labeling with fluorescent dye applied to a second TX caudal or rostral to the original lesion, respectively. We found that over 50% of the early regenerating axons belonged to small neurons in the brainstem. Some propriospinal neurons located close to the TX also contributed to early regeneration. The number of early regenerating propriospinal neurons decreased with distance from the original lesion. Descending axons from the brainstem were labeled anterogradely by application of tracer to a second TX close to the spinal–medullary junction. This limited contamination of the data by regenerating spinal axons whose cell bodies are located rostral or caudal to the TX and confirmed the regeneration of many small RS axons as early as 2 weeks post-TX. Compared with the behavior of axotomized giant axons, the early regenerating axons were of small caliber and showed little retraction, probably because they resealed rapidly after injury.


2020 ◽  
Vol 21 (5) ◽  
pp. 1570 ◽  
Author(s):  
Estrella Calvo ◽  
Santiago Milla-Navarro ◽  
Isabel Ortuño-Lizarán ◽  
Violeta Gómez-Vicente ◽  
Nicolás Cuenca ◽  
...  

Combined administration of N-Methyl-D-Aspartate (NMDA) and kainic acid (KA) on the inner retina was studied as a model of excitotoxicity. The right eye of C57BL6J mice was injected with 1 µL of PBS containing NMDA 30 mM and KA 10 mM. Only PBS was injected in the left eye. One week after intraocular injection, electroretinogram recordings and immunohistochemistry were performed on both eyes. Retinal ganglion cell (RGC) projections were studied by fluorescent-cholerotoxin anterograde labeling. A clear decrease of the retinal “b” wave amplitude, both in scotopic and photopic conditions, was observed in the eyes injected with NMDA/KA. No significant effect on the “a” wave amplitude was observed, indicating the preservation of photoreceptors. Immunocytochemical labeling showed no effects on the outer nuclear layer, but a significant thinning on the inner retinal layers, thus indicating that NMDA and KA induce a deleterious effect on bipolar, amacrine and ganglion cells. Anterograde tracing of the visual pathway after NMDA and KA injection showed the absence of RGC projections to the contralateral superior colliculus and lateral geniculate nucleus. We conclude that glutamate receptor agonists, NMDA and KA, induce a deleterious effect of the inner retina when injected together into the vitreous chamber.


2013 ◽  
Vol 21 (4) ◽  
pp. 10-13 ◽  
Author(s):  
Brendan Brinkman

Researchers have long sought to visualize neural projections and other features of animal brains by imaging them using light microscopes. Connectomics studies strive to elucidate the connections among nerve cells, but brain is dense, and gray matter is tremendously light-scattering. So scientists have not been able to peer as deeply into brain tissue as is necessary to view its complex connections and structures without cutting brain tissue into thin sections. The slicing causes damage that makes it even more difficult to reconstruct exactly how nervous system structures are interconnected. Issues with light scattering have also limited studies of microvasculature, developmental structures, and other features in mouse embryos and other tissues. Traditional methods of retrograde or anterograde labeling have been able to provide high specificity of connection circuitry, but at the price of losing the organizational context of the network. In addition, there is a limit to the practical number of labels that can be used at once. Even multicolor methods have required serial section reconstruction.


2012 ◽  
Vol 525 (2) ◽  
pp. 157-162 ◽  
Author(s):  
Jianhua Zhang ◽  
Mingchu Xi ◽  
Simon J. Fung ◽  
Sharon Sampogna ◽  
Michael H. Chase

2009 ◽  
Vol 1302 ◽  
pp. 76-84 ◽  
Author(s):  
William R. Reed ◽  
Alice Shum-Siu ◽  
Ashley Whelan ◽  
Stephen M. Onifer ◽  
David S.K. Magnuson

2009 ◽  
Vol 178 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Gary C. Walter ◽  
Robert J. Phillips ◽  
Elizabeth A. Baronowsky ◽  
Terry L. Powley

2009 ◽  
Vol 26 (2) ◽  
pp. 215-226 ◽  
Author(s):  
S. H. LINDSTROM ◽  
N. NACSA ◽  
T. BLANKENSHIP ◽  
P. G. FITZGERALD ◽  
C. WELLER ◽  
...  

AbstractThe visual system of birds includes an efferent projection from a visual area, the isthmo-optic nucleus in the midbrain, back to the retina. Using a combination of anterograde labeling of efferent fibers, reconstruction of dye-filled neurons, NADPH-diaphorase staining, and transmission electron microscopy, we have examined the distribution of efferent fibers and their synaptic structures in the chicken retina. We show that efferent fibers terminate strictly within the ventral retina. In two completely mapped retinas, only 2 fibers from a total of 15,359 terminated in the dorsal retina. The major synapse made by each efferent fiber is with a single efferent target amacrine cell (TC). This synapse consists of 5–25 boutons of 2 μm diameter, each with multiple active zones, pressed into the TC soma or synapsing with a basketwork of rudimentary TC dendrites in the inner nuclear layer (INL). This basketwork, which is sheathed by Muller cell processes, defines a private neuropil in the INL within which TCs were also seen to receive input from retinal neurons. In addition to the major synapse, efferent fibers typically produce several very thin processes that terminate nearby in single small boutons and for which the soma of a local amacrine cell is one of the likely postsynaptic partners. A minority of efferent fibers also give rise to a thicker process, terminating in a strongly diaphorase-positive ball about 5 μm in diameter.


2008 ◽  
Vol 294 (4) ◽  
pp. G855-G867 ◽  
Author(s):  
Nick J. Spencer ◽  
Aoife Kerrin ◽  
Vladimir P. Zagorodnyuk ◽  
Grant W. Hennig ◽  
Melodie Muto ◽  
...  

The mechanosensitive endings of low-threshold, slowly adapting pelvic afferents that innervate the rectum have been previously identified as rectal intraganglionic laminar endings (rIGLEs) that lie within myenteric ganglia. We tested whether the aganglionic rectum of piebald-lethal (sl/sl) mice lacks rIGLEs and whether this could explain impaired distension-evoked reflexes from this region. Extracellular recordings were made from fine rectal nerves in C57BL/6 wild-type and sl/sl mice, combined with anterograde labeling. In C57BL/6 mice, graded circumferential stretch applied to the rectum activated graded increases in firing of slowly adapting rectal mechanoreceptors. In sl/sl mice, graded stretch of the aganglionic rectum activated similar graded increases in rectal afferent firing. Stretch-sensitive afferents responded at low mechanical thresholds and fired more intensely at noxious levels of stretch. They could also be activated by probing their receptive fields with von Frey hairs and by muscle contraction. Anterograde labeling from recorded rectal nerves identified the mechanoreceptors of muscular afferents in the aganglionic rectal smooth muscle. A population of afferents were also recorded in both C57BL/6 and sl/sl mice that were activated by von Frey hair probing, but not stretch. In summary, the aganglionic rectum is innervated by a population of stretch-sensitive rectal afferent mechanoreceptor which develops and functions in the absence of any enteric ganglia. These results suggest that in patients with Hirschsprung's disease the inability to activate extrinsic distension reflexes from the aganglionic rectum is unlikely to be due to the absence of stretch-sensitive extrinsic mechanoreceptors.


2007 ◽  
Vol 70 (5) ◽  
pp. 297-301 ◽  
Author(s):  
Riichi Shibata-Iwasaki ◽  
Hideyuki Dekimoto ◽  
Yu Katsuyama ◽  
Satoshi Kikkawa ◽  
Toshio Terashima

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