Synthesis of functional bovine opsin in insect cells under control of the baculovirus polyhedrin promoter

1988 ◽  
Vol 13 (2) ◽  
pp. 65-71 ◽  
Author(s):  
J. J. M. Janssen ◽  
W. J. M. van de Ven ◽  
W. A. H. M. van Groningen-Luyben ◽  
J. Roosien ◽  
J. M. Vlak ◽  
...  
Keyword(s):  
Insect Cells ◽  
Polyhedrin Promoter

scholarly journals Production of human c-myc protein in insect cells infected with a baculovirus expression vector.

1985 ◽  
Vol 5 (10) ◽  
pp. 2860-2865 ◽  
Author(s):  
C Miyamoto ◽  
G E Smith ◽  
J Farrell-Towt ◽  
R Chizzonite ◽  
M D Summers ◽  
...  
Keyword(s):  
Insect Cells ◽  
Immunoblot Analysis ◽  
Subcellular Fractionation ◽  
Autographa Californica ◽  
Baculovirus Expression ◽  
Polyhedrin Promoter ◽  
Baculovirus Expression Vector ◽  
Myc Gene ◽  
Infected Insect ◽  
Nuclear Polyhedrosis

A cDNA fragment coding for human c-myc was inserted into the genome of the baculovirus Autographa californica nuclear polyhedrosis virus adjacent to the strong polyhedrin promoter. Insect cells infected with the recombinant virus produced significant amounts of c-myc protein, which constituted the major phosphoprotein component in these cells. By immunoprecipitation and immunoblot analysis, two proteins of 61 and 64 kilodaltons were detected with c-myc-specific antisera. The insect-derived proteins were compared with recombinant human c-myc-encoded proteins synthesized in Escherichia coli and Saccharomyces cerevisiae cells. The c-myc gene product was found predominantly in the nucleus by subcellular fractionation of infected insect cells.

Potato leafroll virus protein P1 contains a serine proteinase domain

Microbiology ◽  
2000 ◽  
Vol 81 (7) ◽  
pp. 1857-1864 ◽  
Author(s):  
X. Li ◽  
M. D. Ryan ◽  
J. W. Lamb
Keyword(s):  
Insect Cells ◽  
Plant Cells ◽  
Serine Proteinase ◽  
Potato Leafroll Virus ◽  
Autographa Californica ◽  
Virus Protein ◽  
Conserved Residues ◽  
Intermolecular Reaction ◽  
Polyhedrin Promoter ◽  
Leafroll Virus

The multi-domain potato leafroll virus replicase protein P1 was expressed in insect cells from the polyhedrin promoter of Autographa californica nucleopolyhedrovirus. Using antisera raised against P1, it was shown that P1 was cleaved near the VPg in insect cells in a manner similar to that in plant cells, to produce a ∼27 kDa C-terminal fragment. Furthermore, it was shown that the proposed serine proteinase-like domain within P1 is responsible for this processing and that this can occur in a trans (intermolecular) reaction. Four conserved residues within the serine proteinase domain that are essential for catalysis have been identified, consistent with the proposal that this domain comprises a serine proteinase.

scholarly journals Production of human c-myc protein in insect cells infected with a baculovirus expression vector

1985 ◽  
Vol 5 (10) ◽  
pp. 2860-2865
Author(s):  
C Miyamoto ◽  
G E Smith ◽  
J Farrell-Towt ◽  
R Chizzonite ◽  
M D Summers ◽  
...  
Keyword(s):  
Insect Cells ◽  
Immunoblot Analysis ◽  
Subcellular Fractionation ◽  
Autographa Californica ◽  
Baculovirus Expression ◽  
Polyhedrin Promoter ◽  
Baculovirus Expression Vector ◽  
Myc Gene ◽  
Infected Insect ◽  
Nuclear Polyhedrosis

A cDNA fragment coding for human c-myc was inserted into the genome of the baculovirus Autographa californica nuclear polyhedrosis virus adjacent to the strong polyhedrin promoter. Insect cells infected with the recombinant virus produced significant amounts of c-myc protein, which constituted the major phosphoprotein component in these cells. By immunoprecipitation and immunoblot analysis, two proteins of 61 and 64 kilodaltons were detected with c-myc-specific antisera. The insect-derived proteins were compared with recombinant human c-myc-encoded proteins synthesized in Escherichia coli and Saccharomyces cerevisiae cells. The c-myc gene product was found predominantly in the nucleus by subcellular fractionation of infected insect cells.

Enhanced gene expression in insect cells and silkworm larva by modified polyhedrin promoter using repeated burst sequence and very late transcriptional factor-1

2010 ◽  
Vol 107 (6) ◽  
pp. 909-916 ◽  
Author(s):  
Suganthi Lavender Manohar ◽  
Shin Kanamasa ◽  
Takuya Nishina ◽  
Tatsuya Kato ◽  
Enoch Y. Park
Keyword(s):  
Gene Expression ◽  
Insect Cells ◽  
Transcriptional Factor ◽  
Polyhedrin Promoter ◽  
Silkworm Larva

Evaluation of the nucleopolyhedrovirus of Anticarsia gemmatalis as a vector for gene therapy in mammals

2020 ◽  
Vol 20 ◽  
Author(s):  
Cintia N. Parsza ◽  
Diego L. Mengual Gómez ◽  
Jorge Alejandro Simonin ◽  
Mariano Nicolás Belaich ◽  
Pablo Daniel Ghiringhelli
Keyword(s):  
Gene Therapy ◽  
Mammalian Cells ◽  
Insect Cells ◽  
Autographa Californica ◽  
Anticarsia Gemmatalis ◽  
Agricultural Pests ◽  
Mammalian Cell Lines ◽  
Delivery Vector ◽  
Wide Range ◽  
Insect Pathogens

Background: Baculoviruses are insect pathogens with important biotechnological applications that transcend their use as biological controllers of agricultural pests. One species, Autographa californica multiple nucleopolhyedrovirus (AcMNPV) has been extensively exploited as a molecular platform to produce recombinant proteins and as a delivery vector for genes in mammals, because it can transduce a wide range of mammalian cells and tissues without replicating or producing progeny. Objective/Method: To investigate if the budded virions of Anticarsia gemmatalis multiple nucleopolhyedrovirus (AgMNPV) species has the same ability, the viral genome was modified by homologous recombination into susceptible insect cells to integrate reporter genes and then it was evaluated on mammalian cell lines in comparative form with respect to equivalent viruses derived from AcMNPV. Besides, the replicative capacity of AgMNPV´s virions in mammals was determined. Results: The experiments carried out showed that the recombinant variant of AgMNPV transduces and support the expression of delivered genes but not replicates in mammalian cells. Conclusion: Consequently, this insect pathogen is proposed as an alternative of non-infectious viruses in humans to explore new approaches in gene therapy and other applications based on the use of mammalian cells.

Altered phosphorylation pattern of simian virus 40 T antigen expressed in insect cells by using a baculovirus vector.

1990 ◽  
Vol 64 (10) ◽  
pp. 4799-4807 ◽  
Author(s):  
A Höss ◽  
I Moarefi ◽  
K H Scheidtmann ◽  
L J Cisek ◽  
J L Corden ◽  
...  
Keyword(s):  
Insect Cells ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Baculovirus Vector ◽  
Phosphorylation Pattern
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