Enhanced gene expression in insect cells and silkworm larva by modified polyhedrin promoter using repeated burst sequence and very late transcriptional factor-1

2010 ◽  
Vol 107 (6) ◽  
pp. 909-916 ◽  
Author(s):  
Suganthi Lavender Manohar ◽  
Shin Kanamasa ◽  
Takuya Nishina ◽  
Tatsuya Kato ◽  
Enoch Y. Park
Keyword(s):  
Gene Expression ◽  
Insect Cells ◽  
Transcriptional Factor ◽  
Polyhedrin Promoter ◽  
Silkworm Larva

scholarly journals Involvement of microRNA in Solid Cancer: Role and Regulatory Mechanisms

Biomedicines ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 343
Author(s):  
Ying-Chin Lin ◽  
Tso-Hsiao Chen ◽  
Yu-Min Huang ◽  
Po-Li Wei ◽  
Jung-Chun Lin
Keyword(s):  
Gene Expression ◽  
Regulatory Mechanism ◽  
Transcriptional Factor ◽  
Regulatory Mechanisms ◽  
Solid Cancer ◽  
Detailed Knowledge ◽  
Altered Expression ◽  
Human Malignancy ◽  
Regulated Expression ◽  
Pivotal Mechanism

MicroRNAs (miRNAs) function as the post-transcriptional factor that finetunes the gene expression by targeting to the specific candidate. Mis-regulated expression of miRNAs consequently disturbs gene expression profile, which serves as the pivotal mechanism involved in initiation or progression of human malignancy. Cancer-relevant miRNA is potentially considered the therapeutic target or biomarker toward the precise treatment of cancer. Nevertheless, the regulatory mechanism underlying the altered expression of miRNA in cancer is largely uncovered. Detailed knowledge regarding the influence of miRNAs on solid cancer is critical for exploring its potential of clinical application. Herein, we elucidate the regulatory mechanism regarding how miRNA expression is manipulated and its impact on the pathogenesis of distinct solid cancer.

Reproducible and Easy Production of Mammalian Proteins by Transient Gene Expression in High Five Insect Cells

2021 ◽  
pp. 129-140
Author(s):  
Maren Schubert ◽  
Manfred Nimtz ◽  
Federico Bertoglio ◽  
Stefan Schmelz ◽  
Peer Lukat ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insect Cells ◽  
Transient Gene Expression ◽  
Mammalian Proteins

scholarly journals Efficient production of antibody Fab fragment by transient gene expression in insect cells

2017 ◽  
Vol 124 (2) ◽  
pp. 221-226 ◽  
Author(s):  
Keita Mori ◽  
Hirotsugu Hamada ◽  
Takafumi Ogawa ◽  
Yuki Ohmuro-Matsuyama ◽  
Tomohisa Katsuda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insect Cells ◽  
Transient Gene Expression ◽  
Efficient Production ◽  
Fab Fragment

scholarly journals Oncogenic Mutants of RON and MET Receptor Tyrosine Kinases Cause Activation of the β-Catenin Pathway

2001 ◽  
Vol 21 (17) ◽  
pp. 5857-5868 ◽  
Author(s):  
Alla Danilkovitch-Miagkova ◽  
Alexei Miagkov ◽  
Alison Skeel ◽  
Noboru Nakaigawa ◽  
Berton Zbar ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tyrosine Kinases ◽  
Receptor Tyrosine Kinases ◽  
Cell Transformation ◽  
Transcriptional Factor ◽  
Adenomatous Polyposis ◽  
Polyposis Coli ◽  
Met Receptor ◽  
Constitutive Activation ◽  
Oncogenic Protein

ABSTRACT β-Catenin is an oncogenic protein involved in regulation of cell-cell adhesion and gene expression. Accumulation of cellular β-catenin occurs in many types of human cancers. Four mechanisms are known to cause increases in β-catenin: mutations of β-catenin, adenomatous polyposis coli, or axin genes and activation of Wnt signaling. We report a new cause of β-catenin accumulation involving oncogenic mutants of RON and MET receptor tyrosine kinases (RTKs). Cells transfected with oncogenic RON or MET were characterized by β-catenin tyrosine phosphorylation and accumulation; constitutive activation of a Tcf transcriptional factor; and increased levels of β-catenin/Tcf target oncogene proteins c-mycand cyclin D1. Interference with the β-catenin pathway reduced the transforming potential of mutated RON and MET. Activation of β-catenin by oncogenic RON and MET constitutes a new pathway, which might lead to cell transformation by these and other mutant growth factor RTKs.

ECDYSONE-INDUCIBLE FOREIGN GENE EXPRESSION IN STABLY-TRANSFORMED LEPIDOPTERAN INSECT CELLS

2001 ◽  
Vol 37 (9) ◽  
pp. 564 ◽  
Author(s):  
SHUICHIRO TOMITA ◽  
YUKIE KAWAI ◽  
SOO DONG WOO ◽  
MANABU KAMIMURA ◽  
KIKUO IWABUCHI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insect Cells ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Lepidopteran Insect

A Sensitive Model System for In Vivo Monitoring of Baculovirus Gene Expression in Single Infected Insect Cells

1992 ◽  
Vol 10 (5) ◽  
pp. 565-569 ◽  
Author(s):  
Matti Karp ◽  
Karl Åkerman ◽  
Christer Lindqvist ◽  
Ari Kuusisto ◽  
Petri Saviranta ◽  
...  
Keyword(s):  
Gene Expression ◽  
Insect Cells ◽  
Model System ◽  
In Vivo Monitoring ◽  
Baculovirus Gene ◽  
Infected Insect

Baculoviruses as vectors for foreign gene expression in insect cells

1990 ◽  
Vol 28 (2) ◽  
pp. 215-224 ◽  
Author(s):  
Allan E. Atkinson ◽  
Matthew D. Weitzman ◽  
Louis Obosi ◽  
David J. Beadle ◽  
Linda A. King
Keyword(s):  
Gene Expression ◽  
Insect Cells ◽  
Foreign Gene ◽  
Foreign Gene Expression

Synthesis of functional bovine opsin in insect cells under control of the baculovirus polyhedrin promoter

1988 ◽  
Vol 13 (2) ◽  
pp. 65-71 ◽  
Author(s):  
J. J. M. Janssen ◽  
W. J. M. van de Ven ◽  
W. A. H. M. van Groningen-Luyben ◽  
J. Roosien ◽  
J. M. Vlak ◽  
...  
Keyword(s):  
Insect Cells ◽  
Polyhedrin Promoter
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