Detection of the ergosterol and episterol isomers lichesterol and fecosterol in nystatin-resistant mutants of Neurospora crassa

1974 ◽  
Vol 12 (6) ◽  
pp. 459-466 ◽  
Author(s):  
D. C. Morris ◽  
S. Safe ◽  
R. E. Subden
1974 ◽  
Vol 133 (2) ◽  
pp. 123-133 ◽  
Author(s):  
Maria Leonor Teles Grilo ◽  
Walter Klingmüller

1989 ◽  
Vol 35 (3) ◽  
pp. 359-365 ◽  
Author(s):  
Wendy B. Levine ◽  
George A. Marzluf

This study identified and characterized four cadmium-resistant mutants of Neurospora crassa. One of these mutants maps to linkage group II and the other three map to linkage group VII, whereas a naturally occurring resistant trait in a strain from Japan resides at a distinct but unmapped locus. Transport of cadmium into Neurospora cells occurs by more than a single uptake system and involves both energy-dependent and -independent components. The resistant mutants transport cadmium in the same manner as does the cadmium-sensitive wild-type strain. Cadmium resistance in these mutants does not appear to result from an increase in cytosolic heat-stable cadmium-binding proteins. Cadmium does not induce the typical heat-shock response in conidia. Under various growth conditions, each of the mutants exhibited morphological alterations, possibly involving the cell wall or plasma membrane.Key words: cadmium, Neurospora, transport, resistant mutants.


1989 ◽  
Vol 171 (1) ◽  
pp. 53-58 ◽  
Author(s):  
K E Allen ◽  
M T McNally ◽  
H S Lowendorf ◽  
C W Slayman ◽  
S J Free

1963 ◽  
Vol 4 (1) ◽  
Author(s):  
W. Klingmüller ◽  
F. Kaudewitz

1973 ◽  
Vol 15 (4) ◽  
pp. 831-844 ◽  
Author(s):  
G. R. Hoffmann ◽  
H. V. Malling ◽  
T. J. Mitchell

Mutants resistant to inhibitory concentrations of 5-fluorodeoxyuridine (FdUrd) were induced by ultraviolet light. Resistance was demonstrated by growth-tube experiments and by plating efficiencies in the presence and in the absence of FdUrd. Linkage studies involving five mutants revealed the existence of at least two loci conferring resistance to the inhibitor. These loci, designated fdu-1 and fdu-2, were assigned to Linkage Groups VII and IV, respectively. Both resistance genes are recessive to their sensitive alleles in heterokaryons. Resistance to FdUrd is stable both in the presence and in the absence of the inhibitor. Possible mechanisms of resistance to FdUrd are discussed.


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