scholarly journals Isolation and characterization of cadmium-resistant mutants of Neurospora crassa

1989 ◽  
Vol 35 (3) ◽  
pp. 359-365 ◽  
Author(s):  
Wendy B. Levine ◽  
George A. Marzluf
Keyword(s):  
Linkage Group ◽  
Neurospora Crassa ◽  
Growth Conditions ◽  
Uptake System ◽  
Cadmium Resistance ◽  
Morphological Alterations ◽  
Heat Stable ◽  
Isolation And Characterization ◽  
Resistant Mutants ◽  
Energy Dependent

This study identified and characterized four cadmium-resistant mutants of Neurospora crassa. One of these mutants maps to linkage group II and the other three map to linkage group VII, whereas a naturally occurring resistant trait in a strain from Japan resides at a distinct but unmapped locus. Transport of cadmium into Neurospora cells occurs by more than a single uptake system and involves both energy-dependent and -independent components. The resistant mutants transport cadmium in the same manner as does the cadmium-sensitive wild-type strain. Cadmium resistance in these mutants does not appear to result from an increase in cytosolic heat-stable cadmium-binding proteins. Cadmium does not induce the typical heat-shock response in conidia. Under various growth conditions, each of the mutants exhibited morphological alterations, possibly involving the cell wall or plasma membrane.Key words: cadmium, Neurospora, transport, resistant mutants.

Die Aufnahme der Zucker Sorbose, Fructose und Glucose durch Sorbose-resistente Mutanten von Neurospora crassa

1967 ◽  
Vol 22 (3) ◽  
pp. 327-335 ◽  
Author(s):  
Walter Klingmüller
Keyword(s):  
Neurospora Crassa ◽  
Nitrous Acid ◽  
Acid Treatment ◽  
Chromosome Complement ◽  
Sugar Metabolism ◽  
Type I ◽  
Linkage Groups ◽  
Saturation Kinetics ◽  
Resistant Mutants ◽  
Energy Dependent

Sorbose-resistant mutants of Neurospora crassa, arising spontaneously or after nitrous acid treatment, can be mapped in at least 6 separate geneloci, scattered over the chromosome complement. Neither combined enzymatic optic tests, nor radio-paperchromatography give evidence for altered enzymes of sugar metabolism or gain of the ability to phosphorylate and utilize sorbose in these mutants.The increase of radioactivity of germinated conidia as a function of time of incubation with 14C-labelled sorbose revealed that the mutants have a decreased rate of active (energy-dependent) sorbose uptake as compared to the wildtype. No difference in rate of either fructose or glucose uptake between mutants and wildtype could be found.Uptake of sorbose exhibited saturation kinetics for mutants and wildtype. Km values of the mutants were nearly equal to that of the wildtype; there are indications that Vmax is lowered in some of them. If supernatants of conidia of 2 mutants (sorr Α-1 and sorrB-57), mapping in separate linkage groups and thus representing two separate genes, were added to pregerminated wildtype conidia, their rate of sorbose-uptake was not depressed. These 2 mutants are therefore not of the excretor-type, i. e. they must be transport-defective mutants. Data available so far exclude that the mutants have a defect in a common carbohydrate carrier, or in a carrier specific for sorbose transport. It is concluded that mutants sorr Α-1 and sorr B-57 are permease-mutants, with genes A and B coding for 2 separate permeases or permease-subunits.

scholarly journals COMPLEMENTATION ANALYSIS OF LINKED CIRCADIAN CLOCK MUTANTS OF NEUROSPORA CRASSA

Genetics ◽  
1976 ◽  
Vol 82 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Jerry F Feldman ◽  
Marian N Hoyle
Keyword(s):  
Linkage Group ◽  
Circadian Clock ◽  
Neurospora Crassa ◽  
Period Length ◽  
Complementation Analysis ◽  
Wild Type ◽  
The Right

ABSTRACT A fourth mutant of Neurospora crassa, designated frq-4, has been isolated in which the period length of the circadian conidiation rhythm is shortened to 19.3 ± 0.3 hours. This mutant is tightly linked to the three previously isolated frq mutants, and all four map to the right arm of linkage group VII about 10 map units from the centromere. Complementation tests suggest, but do not prove, that all four mutations are allelic, since each of the four mutants is co-dominant with the frq  + allele—i.e., heterokaryons have period lengths intermediate between the mutant and wild-type—and since heterokaryons between pairs of mutants also have period lengths intermediate between those of the two mutants.

Purification and partial amino acid sequence of thuricin S, a new anti-Listeriabacteriocin from Bacillus thuringiensis

2007 ◽  
Vol 53 (2) ◽  
pp. 284-290 ◽  
Author(s):  
Sonia Chehimi ◽  
François Delalande ◽  
Sophie Sablé ◽  
Mohamed-Rabeh Hajlaoui ◽  
Alain Van Dorsselaer ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Proteinase K ◽  
Terminal Sequence ◽  
Partial Amino Acid Sequence ◽  
Ph Values ◽  
Heat Stable ◽  
Isolation And Characterization ◽  
Terminal Amino ◽  
Edman Sequencing

We report the isolation and characterization of a new bacteriocin, thuricin S, produced by the Bacillus thuringiensis subsp. entomocidus HD198 strain. This antibacterial activity is sensitive to proteinase K, is heat-stable, and is stable at a variety of pH values (3–10.5). The monoisotopic mass of thuricin S purified by high perfomance liquid chromatography, as determined with mass spectrometry ESI-TOF-MS, is 3137.61 Da. Edman sequencing and NanoESI-MS/MS experiments provided the sequence of the 18 N-terminal amino acids. Interestingly, thuricin S has the same N-terminal sequence (DWTXWSXL) as bacthuricin F4 and thuricin 17, produced by B. thuringiensis strains BUPM4 and NEB17, respectively, and could therefore be classified as a new subclass IId bacteriocin.

Isolation and characterization of the heat stable enterotoxin from a pathogenic bovine strain of Escherichia coli

1984 ◽  
Vol 9 (4) ◽  
pp. 399-414 ◽  
Author(s):  
Charles Gerday ◽  
Marianne Herman ◽  
Jacques Olivy ◽  
Nicole Gerardin-Otthiers ◽  
Dominique Art ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heat Stable ◽  
Isolation And Characterization ◽  
Bovine Strain

Isolation and Characterization of Apoptosis-Resistant Mutants from a Radiosensitive Mouse Lymphoma Cell Line

1998 ◽  
Vol 149 (1) ◽  
pp. 41 ◽  
Author(s):  
Hidehiko Kawai ◽  
Yukika Kitamura ◽  
Osamu Nikaido ◽  
Masaaki Tatsuka ◽  
Hiroko Hama-Inaba ◽  
...  
Keyword(s):  
Cell Line ◽  
Lymphoma Cell ◽  
Lymphoma Cell Line ◽  
Isolation And Characterization ◽  
Resistant Mutants ◽  
Mouse Lymphoma
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