Comparative studies of hepatotoxicity and fumonisin B1 and B2 content of water and chloroform/methanol extracts of Fusarium moniliforme strain MRC 826 culture material

1990 ◽  
Vol 112 (2) ◽  
pp. 81-92 ◽  
Author(s):  
Kenneth A. Voss ◽  
Ronald D. Plattner ◽  
Charles W. Bacon ◽  
William P. Norred
1995 ◽  
Vol 74 (2) ◽  
pp. 306-313 ◽  
Author(s):  
L.F. KUBENA ◽  
T.S. EDRINGTON ◽  
C. KAMPS-HOLTZAPPLE ◽  
R.B. HARVEY ◽  
M.H. ELISSALDE ◽  
...  

1993 ◽  
Vol 72 (3) ◽  
pp. 456-466 ◽  
Author(s):  
T.S. WEIBKING ◽  
D.R. LEDOUX ◽  
A.J. BERMUDEZ ◽  
J.R. TURK ◽  
G.E. ROTTINGHAUS ◽  
...  

1997 ◽  
Vol 76 (2) ◽  
pp. 256-264 ◽  
Author(s):  
LF Kubena ◽  
TS Edrington ◽  
RB Harvey ◽  
TD Phillips ◽  
AB Sarr ◽  
...  

1994 ◽  
Vol 77 (2) ◽  
pp. 522-525 ◽  
Author(s):  
Paul E Nelson ◽  
Jean H Juba ◽  
P Frank Ross ◽  
Larry G Rice

Abstract Fumonisins were produced by strains of Fusarium moniliforme and F. proliferatum on a medium consisting of 500 g yellow corn kernels and 500 mL distilled water added to a 30.5 × 61 cm autoclavable polyethylene bag. The corn was inoculated by drawing a suspension from a lyophilized culture into a sterile 5 mL syringe fitted with a 19 gauge needle and injecting 1 mL through the side of each polyethylene culture bag. Bags of inoculated com were incubated in the dark at 20° to 22°C for 4 weeks. Seven to 8 days after inoculation, holes were punched near the tops of the bags to promote aeration. After a 4 week incubation, cultures were soaked in chloroform–acetone (50 + 50, v/v) in 4 L flasks overnight to kill fungus and to remove water. Next, the culture media was filtered through 2 mm nylon mesh screens and air dried from 24 to 48 h. Fumonisin concentrations were determined by liquid chromatography/o-phthalaldialdehyde fluorescence. Confirmation was by gas chromatography/mass spectrometry. We observed that the 3 most important factors in the production of fumonisins in bulk corn cultures were temperature control, moisture, and aeration. Extraction by acetonitrile–water (50 + 50, v/v) for 30 min produced the highest yields of fumonisins. Measurable concentrations were reduced by as much as 50% when culture material was heated at 50°C overnight. Fusarium moniliforme strains consistently produced fumonisin B1 as the major component, but some strains of F. proliferatum produced fumonisin B2 and/or fumonisin B3 at higher concentrations than fumonisin B1. Results were calculated on the basis of dried culture material.


Toxicology ◽  
2001 ◽  
Vol 163 (1) ◽  
pp. 11-22 ◽  
Author(s):  
J.D Bailly ◽  
G Benard ◽  
J.-Y Jouglar ◽  
S Durand ◽  
P Guerre

1995 ◽  
Vol 74 (8) ◽  
pp. 1295-1303 ◽  
Author(s):  
L.F. KUBENA ◽  
T.S. EDRINGTON ◽  
C. KAMPS-HOLTZAPPLE ◽  
R.B. HARVEY ◽  
M.H. ELISSALDE ◽  
...  

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