Genetic mapping of arg, cpa, car and tsm genes in Saccharomyces cerevisiae by trisomic analysis

1982 ◽  
Vol 6 (2) ◽  
pp. 93-98 ◽  
Author(s):  
F. Hilger ◽  
M. Pr�vot ◽  
R. Mortimer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Mapping ◽  
Trisomic Analysis

Cloning and genetic mapping of SNF1, a gene required for expression of glucose-repressible genes in Saccharomyces cerevisiae

1984 ◽  
Vol 4 (1) ◽  
pp. 49-53
Author(s):  
J L Celenza ◽  
M Carlson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Mapping ◽  
Gene Product ◽  
Structural Gene ◽  
Glucose Repression ◽  
Carbon Sources ◽  
Recombinant Plasmids ◽  
The Right ◽  
Integrating Vector

A functional SNF1 gene product is required to derepress expression of many glucose-repressible genes in Saccharomyces cerevisiae. Strains carrying a snf1 mutation are unable to grow on sucrose, galactose, maltose, melibiose, or nonfermentable carbon sources; utilization of these carbon sources is regulated by glucose repression. The inability of snf1 mutants to utilize sucrose results from failure to derepress expression of the structural gene for invertase at the RNA level. We isolated recombinant plasmids carrying the SNF1 gene by complementation of the snf1 defect in S. cerevisiae. A 3.5-kilobase region is common to the DNA segments cloned in five different plasmids. Transformation of S. cerevisiae with an integrating vector carrying a segment of the cloned DNA resulted in integration of the plasmid at the SNF1 locus. This result indicates that the cloned DNA is homologous to sequences at the SNF1 locus. By mapping a plasmid marker linked to SNF1 in this transformant, we showed that the SNF1 gene is located on chromosome IV. We then mapped snf1 to a position 5.6 centimorgans distal to rna3 on the right arm; snf1 is not extremely closely linked to any previously mapped mutation.

scholarly journals GENETIC MAPPING IN SACCHAROMYCES IV. MAPPING OF TEMPERATURE-SENSITIVE GENES AND USE OF DISOMIC STRAINS IN LOCALIZING GENES

Genetics ◽  
1973 ◽  
Vol 74 (1) ◽  
pp. 33-54
Author(s):  
R K Mortimer ◽  
D C Hawthorne
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Chromosome Number ◽  
Genetic Mapping ◽  
Genetic Markers ◽  
Genetic Maps ◽  
Temperature Sensitive ◽  
Yeast Saccharomyces Cerevisiae ◽  
Haploid Chromosome Number ◽  
Number Of Genes

ABSTRACT Through use of tetrad, random spore, trisomic, and mitotic analysis procedures a large number of genes, including 48 new genetic markers, were studied for their locations on the genetic maps of the yeast Saccharomyces cerevisiae. Eighteen new centromere linked genes were discovered and all but one was located on various ones of the 16 previously-established chromosomes. Five fragments of linked genes were also assigned to chromosomes; four were located on known chromosomes while the fifth determined one arm of a new chromosome. The experiments indicate that seventeen is likely to be the haploid chromosome number in this yeast. Most chromosomes have been established by genetic means to be metacentric and their genetic lengths vary from 5 cM to approximately 400 cM. Functionally-related sets of genes generally were found to be dispersed over the genome.

scholarly journals Genetic mapping of Ty elements in Saccharomyces cerevisiae.

1984 ◽  
Vol 4 (2) ◽  
pp. 329-339 ◽  
Author(s):  
H L Klein ◽  
T D Petes
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Statistical Analysis ◽  
Genetic Mapping ◽  
Transposable Element ◽  
Genetic Map ◽  
Selectable Marker ◽  
Repeated Element ◽  
Ty Elements ◽  
Reciprocal Exchange ◽  
Leu2 Gene

We used transformation to insert a selectable marker at various sites in the Saccharomyces cerevisiae genome occupied by the transposable element Ty. The vector CV9 contains the LEU2+ gene and a portion of the repeated element Ty1-17. Transformation with this plasmid resulted in integration of the vector via a reciprocal exchange using homology at the LEU2 locus or at the various Ty elements that are dispersed throughout the S. cerevisiae genome. These transformants were used to map genetically sites of several Ty elements. The 24 transformants recovered at Ty sites define 19 distinct loci. Seven of these were placed on the genetic map. Two classes of Ty elements were identified in these experiments: a Ty1-17 class and Ty elements different from Ty1-17. Statistical analysis of the number of transformants at each class of Ty elements shows that there is preferential integration of the CV9 plasmid into the Ty1-17 class.

scholarly journals High-Resolution Genetic Mapping With Ordered Arrays of Saccharomyces cerevisiae Deletion Mutants

Genetics ◽  
2002 ◽  
Vol 162 (3) ◽  
pp. 1091-1099 ◽  
Author(s):  
Paul Jorgensen ◽  
Bryce Nelson ◽  
Mark D Robinson ◽  
Yiqun Chen ◽  
Brenda Andrews ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Resolution ◽  
Genetic Mapping ◽  
Large Scale ◽  
Model Organisms ◽  
Deletion Mutants ◽  
Wild Type ◽  
Allele Specific ◽  
Single Allele ◽  
Kinase Signaling Pathway

Abstract We present a method for high-resolution genetic mapping that takes advantage of the ordered set of viable gene deletion mutants, which form a set of colinear markers covering almost every centimorgan of the Saccharomyces cerevisiae genome, and of the synthetic genetic array (SGA) system, which automates the construction of double mutants formed by mating and meiotic recombination. The Cbk1 kinase signaling pathway, which consists minimally of CBK1, MOB2, KIC1, HYM1, and TAO3 (PAG1), controls polarized morphogenesis and activation of the Ace2 transcription factor. Deletion mutations in the Cbk1 pathway genes are tolerated differently by common laboratory strains of S. cerevisiae, being viable in the W303 background but dead in the S288C background. Genetic analysis indicated that the lethality of Cbk1 pathway deletions in the S288C background was suppressed by a single allele specific to the W303 background. SGA mapping (SGAM) was used to locate this W303-specific suppressor to the SSD1 locus, which contains a known polymorphism that appears to compromise SSD1 function. This procedure should map any mutation, dominant or recessive, whose phenotype is epistatic to wild type, that is, a phenotype that can be scored from a mixed population of cells obtained by germination of both mutant and wild-type spores. In principle, SGAM should be applicable to the analysis of multigenic traits. Large-scale construction of ordered mutations in other model organisms would broaden the application of this approach.

scholarly journals Genetic mapping of quantitative phenotypic traits in Saccharomyces cerevisiae

2012 ◽  
Vol 12 (2) ◽  
pp. 215-227 ◽  
Author(s):  
Steve Swinnen ◽  
Johan M Thevelein ◽  
Elke Nevoigt
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Mapping ◽  
Phenotypic Traits
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