Molecular cloning and genetic mapping of the PET494 gene of Saccharomyces cerevisiae

1984 ◽  
Vol 195 (1-2) ◽  
pp. 275-280 ◽  
Author(s):  
Peter P. Müller ◽  
Thomas D. Fox
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Mapping ◽  
Molecular Cloning

Cloning and genetic mapping of SNF1, a gene required for expression of glucose-repressible genes in Saccharomyces cerevisiae

1984 ◽  
Vol 4 (1) ◽  
pp. 49-53
Author(s):  
J L Celenza ◽  
M Carlson
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Genetic Mapping ◽  
Gene Product ◽  
Structural Gene ◽  
Glucose Repression ◽  
Carbon Sources ◽  
Recombinant Plasmids ◽  
The Right ◽  
Integrating Vector

A functional SNF1 gene product is required to derepress expression of many glucose-repressible genes in Saccharomyces cerevisiae. Strains carrying a snf1 mutation are unable to grow on sucrose, galactose, maltose, melibiose, or nonfermentable carbon sources; utilization of these carbon sources is regulated by glucose repression. The inability of snf1 mutants to utilize sucrose results from failure to derepress expression of the structural gene for invertase at the RNA level. We isolated recombinant plasmids carrying the SNF1 gene by complementation of the snf1 defect in S. cerevisiae. A 3.5-kilobase region is common to the DNA segments cloned in five different plasmids. Transformation of S. cerevisiae with an integrating vector carrying a segment of the cloned DNA resulted in integration of the plasmid at the SNF1 locus. This result indicates that the cloned DNA is homologous to sequences at the SNF1 locus. By mapping a plasmid marker linked to SNF1 in this transformant, we showed that the SNF1 gene is located on chromosome IV. We then mapped snf1 to a position 5.6 centimorgans distal to rna3 on the right arm; snf1 is not extremely closely linked to any previously mapped mutation.

Molecular cloning, genetic mapping, and expression analysis of four zebrafish c/ebp genes

Gene ◽  
2001 ◽  
Vol 281 (1-2) ◽  
pp. 43-51 ◽  
Author(s):  
Susan E. Lyons ◽  
Bixiong C. Shue ◽  
Lin Lei ◽  
Andrew C. Oates ◽  
Leonard I. Zon ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Molecular Cloning ◽  
Expression Analysis

scholarly journals Molecular cloning and transcriptional analysis of the start gene CDC25 of Saccharomyces cerevisiae

1986 ◽  
Vol 5 (9) ◽  
pp. 2363-2369 ◽  
Author(s):  
Enzo Martegani ◽  
Maurizio D. Baroni ◽  
Gianni Frascotti ◽  
Lilia Alberghina
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Cloning ◽  
Transcriptional Analysis

scholarly journals Molecular cloning of invasion plasmid antigen (ipa) genes from Shigella flexneri: analysis of ipa gene products and genetic mapping.

1987 ◽  
Vol 169 (6) ◽  
pp. 2561-2569 ◽  
Author(s):  
J M Buysse ◽  
C K Stover ◽  
E V Oaks ◽  
M Venkatesan ◽  
D J Kopecko
Keyword(s):  
Genetic Mapping ◽  
Molecular Cloning ◽  
Shigella Flexneri ◽  
Gene Products
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