Isolation and characterization of yeast mutants auxotrophic for 2′-deoxythymidine 5′-monophosphate

1979 ◽  
Vol 168 (2) ◽  
pp. 141-151 ◽  
Author(s):  
J. G. Little ◽  
R. H. Haynes
Keyword(s):  
Isolation And Characterization ◽  
Yeast Mutants

scholarly journals Isolation and characterization of awamori yeast mutants with l-leucine accumulation that overproduce isoamyl alcohol

2015 ◽  
Vol 119 (2) ◽  
pp. 140-147 ◽  
Author(s):  
Hiroshi Takagi ◽  
Keisuke Hashida ◽  
Daisuke Watanabe ◽  
Ryo Nasuno ◽  
Masataka Ohashi ◽  
...  
Keyword(s):  
Isoamyl Alcohol ◽  
Isolation And Characterization ◽  
Yeast Mutants

Isolation and characterization of yeast mutants blocked in mevalonic acid formation

1984 ◽  
Vol 123 (2) ◽  
pp. 424-430 ◽  
Author(s):  
Madeleine Servouse ◽  
Nicole Mons ◽  
Jean-Louis Baillargeat ◽  
Francis Karst
Keyword(s):  
Mevalonic Acid ◽  
Acid Formation ◽  
Isolation And Characterization ◽  
Yeast Mutants

Isolation and characterization of sake yeast mutants deficient in γ-aminobutyric acid utilization in sake brewing

2004 ◽  
Vol 97 (6) ◽  
pp. 412-418 ◽  
Author(s):  
Toshinari Takahashi ◽  
Akihisa Furukawa ◽  
Shodo Hara ◽  
Haruhiko Mizoguchi
Keyword(s):  
Aminobutyric Acid ◽  
Isolation And Characterization ◽  
Yeast Mutants

Isolation and characterization of sake yeast mutants with enhanced isoamyl acetate productivity

2017 ◽  
Vol 123 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Toshinari Takahashi ◽  
Yusuke Ohara ◽  
Michiyo Sawatari ◽  
Kazuo Sueno
Keyword(s):  
Isoamyl Acetate ◽  
Isolation And Characterization ◽  
Yeast Mutants

Isolation and Characterization of Yeast Mutants Defective in the Dolichol Pathway for N-Glycosylation

1996 ◽  
pp. 219-228
Author(s):  
Jack Roos ◽  
Xu Jin ◽  
Sandra Centoducati ◽  
José Luz ◽  
Natarajan Ramani ◽  
...  
Keyword(s):  
Isolation And Characterization ◽  
Dolichol Pathway ◽  
Yeast Mutants

scholarly journals Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway.

1995 ◽  
Vol 131 (3) ◽  
pp. 591-602 ◽  
Author(s):  
T M Harding ◽  
K A Morano ◽  
S V Scott ◽  
D J Klionsky
Keyword(s):  
Secretory Pathway ◽  
Protein Targeting ◽  
Protein Localization ◽  
Amino Terminal ◽  
Isolation And Characterization ◽  
Alternate Mechanism ◽  
Complementation Groups ◽  
Vacuolar Protein ◽  
Yeast Mutants

In Saccharomyces cerevisiae the vacuolar protein aminopeptidase I (API) is localized to the vacuole independent of the secretory pathway. The alternate targeting mechanism used by this protein has not been characterized. API is synthesized as a 61-kD soluble cytosolic precursor. Upon delivery to the vacuole, the amino-terminal propeptide is removed by proteinase B (PrB) to yield the mature 50-kD hydrolase. We exploited this delivery-dependent maturation event in a mutant screen to identify genes whose products are involved in API targeting. Using antiserum to the API propeptide, we isolated mutants that accumulate precursor API. These mutants, designated cvt, fall into eight complementation groups, five of which define novel genes. These five complementation groups exhibit a specific defect in maturation of API, but do not have a significant effect on vacuolar protein targeting through the secretory pathway. Localization studies show that precursor API accumulates outside of the vacuole in all five groups, indicating that they are blocked in API targeting and/or translocation. Future analysis of these gene products will provide information about the subcellular components involved in this alternate mechanism of vacuolar protein localization.

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