Discrimination of Rhizobium japonicum, Rhizobium lupini, Rhizobium trifolii, Rhizobium leguminosarum and of bacteriods by uptake of 2-ketoglutaric acid, glutamic acid and phosphate

1976 ◽  
Vol 107 (3) ◽  
pp. 257-262 ◽  
Author(s):  
D. Werner ◽  
K. Bergh�user
1970 ◽  
Vol 16 (10) ◽  
pp. 1003-1009 ◽  
Author(s):  
Ryszard Staniewski

Two hundred and thirty strains of Rhizobium trifolii, Rhizobium leguminosarum for pea, vetch, horse bean, and Lathyrus spp., Rhizobium phaseoli and Rhizobium meliloti were subjected to phage typing. On the basis of their sensitivity to phages these strains were divided into three groups: I, II, and III.In group I, consisting of R. trifolii, R. leguminosarum for pea, vetch, and horse bean, and R. phaseoli, 18 phage types were established. Group II included some strains of R. trifolii and R. leguminosarum for pea and vetch. Among them three phage types were distinguished. Group III included R. meliloti strains and one strain of Rhizobium lupini for lupine. In that group 10 phage types were found.


1966 ◽  
Vol 12 (6) ◽  
pp. 1253-1261 ◽  
Author(s):  
P. K. Chhonkar ◽  
N. S. Subba-Rao

Fungi associated with the nodules of nine common legumes were isolated and identified. The nodules had a mycoflora consisting of Cephalosporium, Alternaria, Aspergillus, Penicillium, Rhizopus, Acrothecium, Fusarium, Rhizoctonia, Curvularia, Pythium, and Trichoderma. Mycoflora of nodules of Trifolium alexandrinum was also analyzed in relation to the age of the plant and the volume of nodules. The fungi were screened for antibiotic activity towards six species of rhizobia. The results showed that Rhizobium trifolii, Rhizobium phaseoli, Rhizobium leguminosarum, and Rhizobium japonicum were relatively more susceptible to fungal antibiotics than either Rhizobium meliloti or Rhizobium sp. (cowpea group). Mycocolonization in soil by an isolate of Cephalosporium sp. significantly reduced the fresh weight and nitrogen status of T. alexandrinum.


1977 ◽  
Vol 102 (1) ◽  
pp. 95-104 ◽  
Author(s):  
K. Planque ◽  
I. R. Kennedy ◽  
G. E. de Vries ◽  
A. Quispel ◽  
A. A. N. van Brussel

1979 ◽  
Vol 25 (10) ◽  
pp. 1169-1174 ◽  
Author(s):  
James R. Manhart ◽  
Peter P. Wong

All species of Rhizobium except R. lupini had nitrate reductase activity. Only R. lupini was incapable of growth with nitrate as the sole source of nitrogen. However, the conditions necessary for the induction of nitrate reductase varied among species of Rhizobium. Rhizobium japonicum and some Rhizobium species of the cowpea strains expressed nitrate reductase activities both in the root nodules of appropriate leguminous hosts and when grown in the presence of nitrate. Rhizobium trifolii, R. phaseoli, and R. legnminosarum did not express nitrate reductase activities in the root nodules, but they did express them when grown in the presence of nitrate. In bacteroids of R. japonicum and some strains of cowpea Rhizobium, high N2 fixation activities were accompanied by high nitrate reductase activities. In bacteroids of R. trifolii, R. leguminosarum, and R. phaseoli, high N2 fixation activities were not accompanied by high nitrate reductase activities.


1983 ◽  
Vol 192 (1-2) ◽  
pp. 171-176 ◽  
Author(s):  
C. A. Wijffelman ◽  
E. Pees ◽  
A. A. N. van Brussel ◽  
P. J. J. Hooykaas

1968 ◽  
Vol 19 (6) ◽  
pp. 919 ◽  
Author(s):  
M/S Chowdhury ◽  
KC Marshall ◽  
CA Parker

The growth characteristics of Rhizobium trifolii and R. lupini in a sterilized infertile sandy soil were studied at different temperatures. R. trifolii grew at a faster rate than R. lupini at all temperatures up to 30°C. The optimum temperature for growth for both species was found to be approximately 30°. Temperatures higher than 35° were found to be lethal for both species, the cells of R. trifolii being the more sensitive. In a fertile sandy loam the growth rates of both species were improved, the effect being more pronounced with R. lupini. The presence of the appropriate host plants in the sterilized infertile sand resulted in faster growth rates in both species, particularly R. lupini.


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