Growth yields and the efficiency of oxidative phosphorylation of Paracoccus denitrificans during two- (carbon) substrate-limited growth

1979 ◽  
Vol 121 (3) ◽  
pp. 213-223 ◽  
Author(s):  
H. W. van Verseveld ◽  
J. P. Boon ◽  
A. H. Stouthamer
Keyword(s):  
Oxidative Phosphorylation ◽  
Paracoccus Denitrificans ◽  
Growth Yields ◽  
Carbon Substrate ◽  
Limited Growth

Design and analysis of experiments on double substrate-limited growth of Paracoccus denitrificans

1984 ◽  
Vol 50 (1) ◽  
pp. 102-102
Author(s):  
E. D. Schoen ◽  
M. A. E. van der Heijden ◽  
H. W. van Verseveld ◽  
A. H. Stouthamer ◽  
J. C. Jager
Keyword(s):  
Paracoccus Denitrificans ◽  
Limited Growth ◽  
Double Substrate

scholarly journals Partial diploids of Escherichia coli carrying normal and mutant alleles affecting oxidative phosphorylation

1977 ◽  
Vol 162 (3) ◽  
pp. 665-670 ◽  
Author(s):  
F Gibson ◽  
G B Cox ◽  
J A Downie ◽  
J Radik
Keyword(s):  
Escherichia Coli ◽  
Fluorescence Quenching ◽  
Oxidative Phosphorylation ◽  
Atpase Activity ◽  
Adenosine Triphosphatase ◽  
Growth Yields ◽  
Normal Allele ◽  
Adenosine Triphosphatase Activity

A plasmid was isolated which included the region of the Escherichia coli chromosome carrying the known genes concerned with oxidative phosphorylation (unc genes). This plasmid was used to prepare partial diploids carrying normal unc alleles on the episome and one of the three mutant alleles (unc A401, uncB402 or unc-405) on the chromosome. These strains were compared with segregants from which the plasmid had been lost. Dominance of either normal ormutant unc alleles was determined by growth on succinate, growth yields on glucose, Mg-ATPase (Mg2+-stimulated adenosine triphosphatase) activity, atebrin-fluorescence quenching, ATP-dependent transhydrogenase activity and oxidative phosphorylation. In all the above tests, dominance of the normal allele was observed. However, in membranes from the diploid strains which carried a normal allele and either of the mutant alleles affecting Mg-ATPase activity (uncA401 or unc-405), the energy-linked functions were only partially restored.

scholarly journals Involvement of the cbb3-Type Terminal Oxidase in Growth Competition of Bacteria, Biofilm Formation, and in Switching between Denitrification and Aerobic Respiration

2020 ◽  
Vol 8 (8) ◽  
pp. 1230
Author(s):  
Igor Kučera ◽  
Vojtěch Sedláček
Keyword(s):  
Paracoccus Denitrificans ◽  
Aerobic Denitrification ◽  
Wild Type ◽  
Bacterial Strains ◽  
Aerobic Growth ◽  
Limited Growth ◽  
Terminal Oxidases ◽  
Ubiquinol Oxidase ◽  
Fitness Advantage ◽  
Transport Chain

Paracoccus denitrificans has a branched electron transport chain with three terminal oxidases transferring electrons to molecular oxygen, namely aa3-type and cbb3-type cytochrome c oxidases and ba3-type ubiquinol oxidase. In the present study, we focused on strains expressing only one of these enzymes. The competition experiments showed that possession of cbb3-type oxidase confers significant fitness advantage during oxygen-limited growth and supports the biofilm lifestyle. The aa3-type oxidase was shown to allow rapid aerobic growth at a high oxygen supply. Activity of the denitrification pathway that had been expressed in cells grown anaerobically with nitrate was fully inhibitable by oxygen only in wild-type and cbb3 strains, while in strains aa3 and ba3 dinitrogen production from nitrate and oxygen consumption occurred simultaneously. Together, the results highlight the importance of the cbb3-type oxidase for the denitrification phenotype and suggest a way of obtaining novel bacterial strains capable of aerobic denitrification.

Subcellular and cellular studies on relationship between structure and uncoupling effect of phenylhydrazonopropanedinitriles on oxidative phosphorylation

1988 ◽  
Vol 53 (5) ◽  
pp. 1094-1101 ◽  
Author(s):  
Ernest Šturdík ◽  
Štefan Baláž ◽  
Edita Ďurčová ◽  
Marián Antalík ◽  
Pavol Sulo ◽  
...  
Keyword(s):  
Oxidative Phosphorylation ◽  
Dissociation Constants ◽  
Paracoccus Denitrificans ◽  
Physicochemical Characteristics ◽  
Saturation Curve ◽  
Chemical Parameters ◽  
Thiol Compounds ◽  
Physico Chemical ◽  
Bell Shaped Curve

The characterization of relations between the assumed activity-determining physico-chemical parameters (partition coefficients, dissociation constants, reactivity to thiol compounds) of o-, m-, and p-substituted phenylhydrazonopropanedinitriles and their uncoupling effect on oxidative phosphorylation in mitochondria isolated from rat liver, Paracoccus denitrificans bacteria and animal leukemia P 388 cells have shown that from the viewpoint of action mechanism the concept of proton-translocating (pH-gradient disturbing) effect is more plausible than the modification of the corresponding membrane proteins. The dependence of the activity of the compounds studied on their physicochemical characteristics is represented by a saturation curve with a plateau starting at log P 5-6 when the uncoupling effect is plotted versus lipophilicity and by a bell-shaped curve having a maximum at pKa 4·5-5·5 with respect to basicity.

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