Subcellular and cellular studies on relationship between structure and uncoupling effect of phenylhydrazonopropanedinitriles on oxidative phosphorylation

1988 ◽  
Vol 53 (5) ◽  
pp. 1094-1101 ◽  
Author(s):  
Ernest Šturdík ◽  
Štefan Baláž ◽  
Edita Ďurčová ◽  
Marián Antalík ◽  
Pavol Sulo ◽  
...  

The characterization of relations between the assumed activity-determining physico-chemical parameters (partition coefficients, dissociation constants, reactivity to thiol compounds) of o-, m-, and p-substituted phenylhydrazonopropanedinitriles and their uncoupling effect on oxidative phosphorylation in mitochondria isolated from rat liver, Paracoccus denitrificans bacteria and animal leukemia P 388 cells have shown that from the viewpoint of action mechanism the concept of proton-translocating (pH-gradient disturbing) effect is more plausible than the modification of the corresponding membrane proteins. The dependence of the activity of the compounds studied on their physicochemical characteristics is represented by a saturation curve with a plateau starting at log P 5-6 when the uncoupling effect is plotted versus lipophilicity and by a bell-shaped curve having a maximum at pKa 4·5-5·5 with respect to basicity.

2015 ◽  
Vol 88 (1) ◽  
pp. 15-22 ◽  
Author(s):  
Igor Jerković ◽  
Carlo Ignazio Giovanni Tuberoso ◽  
Goran Baranović ◽  
Zvonimir Marijanović ◽  
Marina Kranjac ◽  
...  

2021 ◽  
Vol 6 (2) ◽  
pp. 18-24
Author(s):  
Shashikala Kokcha ◽  
Harsha Chatrath

Purpose of the study: The purpose of this study is to make sure that the Bird valley’s quarry water is suitable for drinking purposes or not and to monitor the seasonal variations in the physico-chemical parameters of this quarry water. Methodology: Water samples from the quarry were collected in clean and sterilized polyethylene bottles. Water samples were collected from different points and mixed together to get an integrated sample. Some of the selected physico-chemical parameters of the quarry water have been analyzed. Results were compared with standard limits of IS: 10500-2012. All the parameters were analyzed in the laboratory by using standard methods and techniques. Main Findings: As per the obtained results this quarry water contains a very large number of Coliforms detected in the months of September and December and in June month Coliform count was 33 CFU/ml. This overall result for coliform is making this quarry water unfit for domestic purposes. Applications of this study: This study helps us to understand the current condition of this quarry water and also enables us to know whether the quarry water is fit for drinking purposes. It also enables us to know whether this quarry water can be used for domestic purposes after the treatment. Novelty/Originality of this study: Bird valley’s quarry water has not been analyzed from this point of view till date. This study will help us to understand the present condition of the water.


Food Control ◽  
2002 ◽  
Vol 13 (4-5) ◽  
pp. 329-336 ◽  
Author(s):  
Alexandre J. Cichoscki ◽  
Eunice Valduga ◽  
Alice T. Valduga ◽  
Marı́a E. Tornadijo ◽  
José M. Fresno

Our Nature ◽  
2013 ◽  
Vol 10 (1) ◽  
pp. 249-257 ◽  
Author(s):  
M Gadhia ◽  
R Surana ◽  
E Ansari

Present study was carried out to determine the important physico chemical parameters of water of Tapi estuary in Hazira industrial area. Tapi estuary receives the inputs of organic matter and nutrients coming from the domestic wastewater discharges from Surat City, a textile hub as well as industrial effluents from Hazira, a major industrial Complex of Gujarat, India. Uncontrolled discharges of domestic wastes and industrial effluents have affected the estuary. Present study was carried out from June - 2011 to May 2012 to study the impacts of uncontrolled discharges on physicochemical characteristics of water. Samples were collected monthly in the morning during low tide to determine the important physico chemical parameters Viz. Turbidity, conductivity, total solid, total dissolved solid, salinity, pH, dissolved oxygen, BOD, COD, Calcium, chloride, sodium , potassium and nutrients like nitrate, nitrite and phosphate. Dissolved oxygen was found extremely low during the study period. High biochemical oxygen demand (BOD) and Chemical Oxygen demand (COD) showed that the water quality of estuary has been affected by industrial and domestic effluents. DOI: http://dx.doi.org/10.3126/on.v10i1.7811


2018 ◽  
Vol 3 (02) ◽  
pp. 150-157
Author(s):  
Asad Amir ◽  
Neelesh Kapoor ◽  
Hirdesh Kumar ◽  
Mohd. Tariq ◽  
Mohd. Asif Siddiqui

Sandalwood is a commercially and culturally important plant species belonging to the family Santalaceae and the genus Santalum. In Indian sandalwood is renowned for its oil, which is highly rated for its sweet, fragrant, persistent aroma and the fixative property which is highly demanded by the perfume industry. For better production and varieties, requires to understanding the functions of proteins, their analysis and characterization of proteins sequences and their structures, their localizations in cell and their interaction with other functional partner. Due to limited number of in silico studies on sandalwood, in the present study we have performed in silico analysis by characterization of sandalwood proteins. Total 23 proteins were obtained and characterization using UniProtKB, identifying their physico-chemical parameters using ProtParam tool and prediction of their secondary structure elements using GOR of all 23 proteins.


Lectin is a protein which has the ability to bind carbohydrates and named as haemagglutinin. Lectins with specific carbohydrate specificity have been purified from various plant tissues and other organisms and exploited extensively in many aspects of biochemistry and biomedicine. Similar to land plants, lectins from marine algae appear to be useful in some biological applications. Although several studies on lectins from marine algae have been reported till date, few lectins from algae have been characterized in detail. The present study was focused on the lectin isolated from C.sinuosa. The algal lectin has high sugar specificity with N-acetylglucosamine and higher enzyme activity with trypsin. This lectin was identified as CaCl2 dependent – ‘C’ type lectin and was sensitive to EDTA. Higher H.A titre value was observed with CaCl2 and the lower with MnCl2 and ZnCl2 . Significant lectin activity was observed between pH 7 to 8 and temperature between 20 to 40 O C


2005 ◽  
Vol 107 (3) ◽  
pp. 158-166 ◽  
Author(s):  
José Luis Bernal ◽  
Juan José Jiménez ◽  
María Jesús del Nozal ◽  
Laura Toribio ◽  
María Teresa Martín

2020 ◽  
Vol 14 (7) ◽  
pp. 2452-2462
Author(s):  
Ali Mohamed Abdou Salam ◽  
Ngansoumana Ba ◽  
Ismaïla Ndour ◽  
Seyni Sane ◽  
Modou Thiaw ◽  
...  

Au Sénégal, peu d’études sur le plan planctonique ont été réalisées pour l’évaluation de l’efficacité des Aires Marines Protégées. Dans cette perspective, la caractérisation de la flore phytoplanctonique et sa distribution spatio-temporelle à l’intérieur et à l’extérieur de l’AMP de Bamboung a été réalisée en avril et en septembre 2016. Des prélèvements d’échantillons d’eau à l’aide d’un filet à plancton de 20 μm de mailles ainsi que des mesures de paramètres physico-chimiques ont été faits dans chaque station. L’analyse des données environnementales a montré l’existence d’un effet intersaison avec des valeurs plus élevées en avril excepté la température. Par ailleurs, un effet d’inter-sites a été observé uniquement en septembre pour la température, la salinité, la conductivité et l’oxygène dissout. En termes de diversité phytoplanctonique, en avril, 49 espèces réparties dans 5 classes ont été identifiées dans l’AMP contre 47 espèces et 5 classes identifiées à Diomboss et 42 espèces et 5 classes à Sangako. En septembre, 57 espèces réparties dans 5 classes ont été répertoriées dans l’AMP contre 54 espèces et 4 classes à Diomboss, et 51 espèces réparties dans 4 classes identifiées à Sangako. Les résultats seuls de la richesse spécifique du phytoplancton ne permettent pas d’apporter une contribution sur l’évaluation de l’efficacité de l’AMP de Bamboung. Mots clés : Phytoplancton, dynamique, composition taxonomique, AMP, Bamboung.   English Title: Characterization of the phytoplankton flora in the Protected Marine Area (MPA) of Bamboung and two surrounding sites (Senegal) In Senegal, few planktonic data exist in the Marine Protected Areas (AMP). In this perspective, the characterization of phytoplankton flora and its spatial and temporal distribution within and outside the Bamboung AMP was carried out during in April and in september. Water samples were taken using a 20 μm mesh plankton net and measurements of physico-chemical parameters were made at each station. The analysis of environmental data showed the existence of an inter-site effect during the two-month sampling period. In April, 49 species in 5 classes were identified in the AMP, compared to 47 species and 5 classes identified in Diomboss and 42 species and 5 classes in Sangako. In september, 57 species distributed in 5 classes were recorded in the AMP compared to 54 species distributed in 4 classes in Diomboss and 51 species and 4 classes identified in Sangako. The results of the specific richness of phytoplankton alone do not make it possible to make a contribution to the evaluation of the effectiveness of Bamboung's MPA.Keywords: Phytoplankton, dynamics, taxonomic composition, AMP; Bamboung.


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