Growth yields and the efficiency of oxidative phosphorylation during autotrophic growth of Paracoccus denitrificans on methanol and formate

1978 ◽  
Vol 118 (1) ◽  
pp. 21-26 ◽  
Author(s):  
H. W. van Verseveld ◽  
A. H. Stouthamer
Keyword(s):  
Oxidative Phosphorylation ◽  
Paracoccus Denitrificans ◽  
Growth Yields ◽  
Autotrophic Growth

scholarly journals Partial diploids of Escherichia coli carrying normal and mutant alleles affecting oxidative phosphorylation

1977 ◽  
Vol 162 (3) ◽  
pp. 665-670 ◽  
Author(s):  
F Gibson ◽  
G B Cox ◽  
J A Downie ◽  
J Radik
Keyword(s):  
Escherichia Coli ◽  
Fluorescence Quenching ◽  
Oxidative Phosphorylation ◽  
Atpase Activity ◽  
Adenosine Triphosphatase ◽  
Growth Yields ◽  
Normal Allele ◽  
Adenosine Triphosphatase Activity

A plasmid was isolated which included the region of the Escherichia coli chromosome carrying the known genes concerned with oxidative phosphorylation (unc genes). This plasmid was used to prepare partial diploids carrying normal unc alleles on the episome and one of the three mutant alleles (unc A401, uncB402 or unc-405) on the chromosome. These strains were compared with segregants from which the plasmid had been lost. Dominance of either normal ormutant unc alleles was determined by growth on succinate, growth yields on glucose, Mg-ATPase (Mg2+-stimulated adenosine triphosphatase) activity, atebrin-fluorescence quenching, ATP-dependent transhydrogenase activity and oxidative phosphorylation. In all the above tests, dominance of the normal allele was observed. However, in membranes from the diploid strains which carried a normal allele and either of the mutant alleles affecting Mg-ATPase activity (uncA401 or unc-405), the energy-linked functions were only partially restored.

Subcellular and cellular studies on relationship between structure and uncoupling effect of phenylhydrazonopropanedinitriles on oxidative phosphorylation

1988 ◽  
Vol 53 (5) ◽  
pp. 1094-1101 ◽  
Author(s):  
Ernest Šturdík ◽  
Štefan Baláž ◽  
Edita Ďurčová ◽  
Marián Antalík ◽  
Pavol Sulo ◽  
...  
Keyword(s):  
Oxidative Phosphorylation ◽  
Dissociation Constants ◽  
Paracoccus Denitrificans ◽  
Physicochemical Characteristics ◽  
Saturation Curve ◽  
Chemical Parameters ◽  
Thiol Compounds ◽  
Physico Chemical ◽  
Bell Shaped Curve

The characterization of relations between the assumed activity-determining physico-chemical parameters (partition coefficients, dissociation constants, reactivity to thiol compounds) of o-, m-, and p-substituted phenylhydrazonopropanedinitriles and their uncoupling effect on oxidative phosphorylation in mitochondria isolated from rat liver, Paracoccus denitrificans bacteria and animal leukemia P 388 cells have shown that from the viewpoint of action mechanism the concept of proton-translocating (pH-gradient disturbing) effect is more plausible than the modification of the corresponding membrane proteins. The dependence of the activity of the compounds studied on their physicochemical characteristics is represented by a saturation curve with a plateau starting at log P 5-6 when the uncoupling effect is plotted versus lipophilicity and by a bell-shaped curve having a maximum at pKa 4·5-5·5 with respect to basicity.

Autotrophic growth on carbon disulfide is a property of novel strains of Paracoccus denitrificans

1997 ◽  
Vol 168 (3) ◽  
pp. 225-236 ◽  
Author(s):  
Sarah L. Jordan ◽  
I. R. McDonald ◽  
Anna J. Kraczkiewicz-Dowjat ◽  
Donovan P. Kelly ◽  
Frederick A. Rainey ◽  
...  
Keyword(s):  
Carbon Disulfide ◽  
Paracoccus Denitrificans ◽  
Autotrophic Growth
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