The anaerobic degradation of aromatic compounds by a denitrifying bacterium

1972 ◽  
Vol 83 (2) ◽  
pp. 165-171 ◽  
Author(s):  
Barrie F. Taylor ◽  
Mary Jo Heeb
Keyword(s):  
Aromatic Compounds ◽  
Anaerobic Degradation ◽  
Denitrifying Bacterium

scholarly journals Anaerobic Degradation of 4-Methylbenzoate by a Newly Isolated Denitrifying Bacterium, Strain pMbN1

2011 ◽  
Vol 78 (5) ◽  
pp. 1606-1610 ◽  
Author(s):  
Sven Lahme ◽  
Jens Harder ◽  
Ralf Rabus
Keyword(s):  
Aromatic Compounds ◽  
Anaerobic Degradation ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Denitrifying Bacterium ◽  
Reducing Conditions ◽  
Gene Sequence Analysis ◽  
Bacterium Strain

ABSTRACTA novel alphaproteobacterium isolated from freshwater sediments, strain pMbN1, degrades 4-methylbenzoate to CO2under nitrate-reducing conditions. While strain pMbN1 utilizes several benzoate derivatives and other polar aromatic compounds, it cannot degradep-xylene or other hydrocarbons. Based on 16S rRNA gene sequence analysis, strain pMbN1 is affiliated with the genusMagnetospirillum.

Stereochemical Insights into the Anaerobic Degradation of 4‐Isopropylbenzoyl‐CoA in the Denitrifying Bacterium Strain pCyN1

2019 ◽  
Vol 25 (18) ◽  
pp. 4722-4731 ◽  
Author(s):  
Julian Küppers ◽  
Patrick Becker ◽  
René Jarling ◽  
Marvin Dörries ◽  
Nevenka Cakić ◽  
...  
Keyword(s):  
Anaerobic Degradation ◽  
Denitrifying Bacterium ◽  
Bacterium Strain

scholarly journals Solvent Stress Response of the Denitrifying Bacterium “Aromatoleum aromaticum” Strain EbN1

2008 ◽  
Vol 74 (8) ◽  
pp. 2267-2274 ◽  
Author(s):  
Kathleen Trautwein ◽  
Simon Kühner ◽  
Lars Wöhlbrand ◽  
Thomas Halder ◽  
Kenny Kuchta ◽  
...  
Keyword(s):  
Stress Responses ◽  
Aromatic Compounds ◽  
Gel Electrophoresis ◽  
Physiological Responses ◽  
Dry Weight ◽  
Anaerobic Growth ◽  
Solvent Concentration ◽  
Denitrifying Bacterium ◽  
Aromatic Substrates ◽  
Difference Gel Electrophoresis

ABSTRACT The denitrifying betaproteobacterium “Aromatoleum aromaticum” strain EbN1 degrades several aromatic compounds, including ethylbenzene, toluene, p-cresol, and phenol, under anoxic conditions. The hydrophobicity of these aromatic solvents determines their toxic properties. Here, we investigated the response of strain EbN1 to aromatic substrates at semi-inhibitory (about 50% growth inhibition) concentrations under two different conditions: first, during anaerobic growth with ethylbenzene (0.32 mM) or toluene (0.74 mM); and second, when anaerobic succinate-utilizing cultures were shocked with ethylbenzene (0.5 mM), toluene (1.2 mM), p-cresol (3.0 mM), and phenol (6.5 mM) as single stressors or as a mixture (total solvent concentration, 2.7 mM). Under all tested conditions impaired growth was paralleled by decelerated nitrate-nitrite consumption. Additionally, alkylbenzene-utilizing cultures accumulated poly(3-hydroxybutyrate) (PHB) up to 10% of the cell dry weight. These physiological responses were also reflected on the proteomic level (as determined by two-dimensional difference gel electrophoresis), e.g., up-regulation of PHB granule-associated phasins, cytochrome cd1 nitrite reductase of denitrification, and several proteins involved in oxidative (e.g., SodB) and general (e.g., ClpB) stress responses.

Occurrence and diversity of the oxidative hydroxyhydroquinone pathway for the anaerobic degradation of aromatic compounds in nitrate‐reducing bacteria

2019 ◽  
Vol 11 (4) ◽  
pp. 525-537 ◽  
Author(s):  
Daniel Pacheco‐Sánchez ◽  
Ramón Rama‐Garda ◽  
Patricia Marín ◽  
Sophie‐Marie Martirani‐Von Abercron ◽  
Silvia Marqués
Keyword(s):  
Aromatic Compounds ◽  
Anaerobic Degradation ◽  
Reducing Bacteria ◽  
Nitrate Reducing Bacteria

Anaerobic degradation of homocyclic aromatic compounds via arylcarboxyl-coenzyme A esters: organisms, strategies and key enzymes

2013 ◽  
Vol 16 (3) ◽  
pp. 612-627 ◽  
Author(s):  
Matthias Boll ◽  
Claudia Löffler ◽  
Brandon E. L. Morris ◽  
Johannes W. Kung
Keyword(s):  
Aromatic Compounds ◽  
Anaerobic Degradation ◽  
Coenzyme A ◽  
Key Enzymes ◽  
Coenzyme A Esters

Anaerobic degradation of 3-halobenzoates by a denitrifying bacterium

1999 ◽  
Vol 171 (4) ◽  
pp. 230-236 ◽  
Author(s):  
M. M. Häggblom ◽  
L. Y. Young
Keyword(s):  
Anaerobic Degradation ◽  
Denitrifying Bacterium

scholarly journals Anaerobic degradation of syringic acid by an adapted strain of Rhodopseudomonas palustris

2019 ◽  
Author(s):  
J. Zachary Oshlag ◽  
Yanjun Ma ◽  
Kaitlin Morse ◽  
Brian T. Burger ◽  
Rachelle A. Lemke ◽  
...  
Keyword(s):  
Aromatic Compounds ◽  
Anaerobic Degradation ◽  
Fossil Fuels ◽  
Plant Biomass ◽  
Rhodopseudomonas Palustris ◽  
Reducing Power ◽  
Sole Source ◽  
Syringic Acid ◽  
Detailed Knowledge ◽  
Acid Degradation

ABSTRACTWhile lignin represents a major fraction of the carbon in plant biomass, biological strategies to convert the components of this heterogenous polymer into products of industrial and biotechnological value are lacking. Syringic acid (3,5-dimethoxy-4-hydroxybenzoic acid) is a byproduct of lignin degradation, appearing in lignocellulosic hydrolysates, deconstructed lignin streams, and other agricultural products. Rhodopseudomonas palustris CGA009 is a known degrader of phenolic compounds under photoheterotrophic conditions, via the benzoyl-CoA degradation (BAD) pathway. However, R. palustris CGA009 is reported to be unable to metabolize meta-methoxylated phenolics such as syringic acid. We isolated a strain of R. palustris (strain SA008.1.07), adapted from CGA009, which can grow on syringic acid under photoheterotrophic conditions, utilizing it as a sole source of organic carbon and reducing power. An SA008.1.07 mutant with an inactive benzoyl-CoA reductase structural gene was able to grow on syringic acid, demonstrating that the metabolism of this aromatic compound is not through the BAD pathway. Comparative gene expression analyses of SA008.1.07 implicated the involvement of products of the vanARB operon (rpa3619-rpa3621), which has been described as catalyzing aerobic aromatic ring demethylation in other bacteria, in anaerobic syringic acid degradation. In addition, experiments with a vanARB deletion mutant demonstrated the involvement of the vanARB operon in anaerobic syringic acid degradation. These observations provide new insights into the anaerobic degradation of meta-methoxylated and other aromatics by R. palustris.IMPORTANCELignin is the most abundant aromatic polymer on Earth and a resource that could eventually substitute for fossil fuels as a source of aromatic compounds for industrial and biotechnological applications. Engineering microorganisms for production of aromatic-based biochemicals requires detailed knowledge of metabolic pathways for the degradation of aromatics that are present in lignin. Our isolation and analysis of a Rhodopseudomonas palustris strain capable of syringic acid degradation reveals a previously unknown metabolic route for aromatic degradation in R. palustris. This study highlights several key features of this pathway and sets the stage for a more complete understanding of the microbial metabolic repertoire to metabolize aromatic compounds from lignin and other renewable sources.

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