Stereochemical Insights into the Anaerobic Degradation of 4‐Isopropylbenzoyl‐CoA in the Denitrifying Bacterium Strain pCyN1

2019 ◽  
Vol 25 (18) ◽  
pp. 4722-4731 ◽  
Author(s):  
Julian Küppers ◽  
Patrick Becker ◽  
René Jarling ◽  
Marvin Dörries ◽  
Nevenka Cakić ◽  
...  
Keyword(s):  
Anaerobic Degradation ◽  
Denitrifying Bacterium ◽  
Bacterium Strain

scholarly journals Anaerobic Degradation of 4-Methylbenzoate by a Newly Isolated Denitrifying Bacterium, Strain pMbN1

2011 ◽  
Vol 78 (5) ◽  
pp. 1606-1610 ◽  
Author(s):  
Sven Lahme ◽  
Jens Harder ◽  
Ralf Rabus
Keyword(s):  
Aromatic Compounds ◽  
Anaerobic Degradation ◽  
Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Content Type ◽  
Denitrifying Bacterium ◽  
Reducing Conditions ◽  
Gene Sequence Analysis ◽  
Bacterium Strain

ABSTRACTA novel alphaproteobacterium isolated from freshwater sediments, strain pMbN1, degrades 4-methylbenzoate to CO2under nitrate-reducing conditions. While strain pMbN1 utilizes several benzoate derivatives and other polar aromatic compounds, it cannot degradep-xylene or other hydrocarbons. Based on 16S rRNA gene sequence analysis, strain pMbN1 is affiliated with the genusMagnetospirillum.

Genes involved in the anaerobic degradation of toluene in a denitrifying bacterium, strain EbN1

2004 ◽  
Vol 181 (3) ◽  
pp. 182-194 ◽  
Author(s):  
Michael Kube ◽  
Johann Heider ◽  
Judith Amann ◽  
Peter Hufnagel ◽  
Simon K�hner ◽  
...  
Keyword(s):  
Anaerobic Degradation ◽  
Denitrifying Bacterium ◽  
Bacterium Strain

Genes involved in the anaerobic degradation of ethylbenzene in a denitrifying bacterium, strain EbN1

2002 ◽  
Vol 178 (6) ◽  
pp. 506-516 ◽  
Author(s):  
Ralf Rabus ◽  
Michael Kube ◽  
Alfred Beck ◽  
Friedrich Widdel ◽  
Richard Reinhardt
Keyword(s):  
Anaerobic Degradation ◽  
Denitrifying Bacterium ◽  
Bacterium Strain

Anaerobic degradation of 3-halobenzoates by a denitrifying bacterium

1999 ◽  
Vol 171 (4) ◽  
pp. 230-236 ◽  
Author(s):  
M. M. Häggblom ◽  
L. Y. Young
Keyword(s):  
Anaerobic Degradation ◽  
Denitrifying Bacterium

Anaerobic degradation of n -hexane in a denitrifying bacterium: Further degradation of the initial intermediate (1-methylpentyl)succinate via C-skeleton rearrangement

2002 ◽  
Vol 177 (3) ◽  
pp. 235-243 ◽  
Author(s):  
Heinz Wilkes ◽  
Ralf Rabus ◽  
Thomas Fischer ◽  
Antje Armstroff ◽  
Astrid Behrends ◽  
...  
Keyword(s):  
Anaerobic Degradation ◽  
Denitrifying Bacterium

Anaerobic degradation ofm-cresol via methyl oxidation to 3-hydroxybenzoate by a denitrifying bacterium

1995 ◽  
Vol 164 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Cornelus F. C. Bonting ◽  
Sabine Schneider ◽  
Günther Schmidtberg ◽  
Georg Fuchs
Keyword(s):  
Anaerobic Degradation ◽  
Denitrifying Bacterium

scholarly journals Substrate-Dependent Regulation of Anaerobic Degradation Pathways for Toluene and Ethylbenzene in a Denitrifying Bacterium, Strain EbN1

2005 ◽  
Vol 187 (4) ◽  
pp. 1493-1503 ◽  
Author(s):  
Simon Kühner ◽  
Lars Wöhlbrand ◽  
Ingo Fritz ◽  
Wasco Wruck ◽  
Claus Hultschig ◽  
...  
Keyword(s):  
Dna Microarray ◽  
Anaerobic Degradation ◽  
Environmental Concern ◽  
Anaerobic Growth ◽  
Toluene Degradation ◽  
Reverse Transcription Pcr ◽  
Expression Studies ◽  
Difference Gel Electrophoresis ◽  
Bacterium Strain ◽  
Growth Experiments

ABSTRACT Anaerobic biodegradation of toluene and ethylbenzene is of environmental concern and biochemical interest due to toxicity and novel reactions, respectively. The denitrifying strain EbN1 is unique in anaerobically degrading both alkylbenzenes via different pathways which converge at benzoyl coenzyme A. The organization of genes involved in both pathways was only recently determined for strain EbN1. In the present study, global expression analysis (DNA microarray and proteomics) indicated involvement of several thus-far-unknown proteins in the degradation of both alkylbenzenes. For example, orf68 and orf57, framing the ebd operon, are implicated in ethylbenzene degradation, and the ebA1932 and ebA1936 genes, located 7.2 kb upstream of the bbs operon, are implicated in toluene degradation. In addition, expression studies were now possible on the level of the complete pathways. Growth experiments demonstrated that degradative capacities for toluene and ethylbenzene could be simultaneously induced, regardless of the substrate used for adaptation. Regulation was studied at the RNA (real-time reverse transcription-PCR and DNA microarray) and protein (two-dimensional-difference gel electrophoresis) level by using cells adapted to anaerobic growth with benzoate, toluene, ethylbenzene, or a mixture of toluene and ethylbenzene. Expression of the two toluene-related operons (bss and bbs) was specifically induced in toluene-adapted cells. In contrast, genes involved in anaerobic ethylbenzene degradation were induced in ethylbenzene- and toluene-adapted cells, suggesting that toluene may act as a gratuitous inducer. In agreement with the predicted sequential regulation of the ethylbenzene pathway, Ebd proteins (encoding subunits of ethylbenzene dehydrogenase) were formed in ethylbenzene- but not in acetophenone-adapted cells, while Apc proteins (subunits of predicted acetophenone carboxylase) were formed under both conditions.

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