The overproducing CYP1 and the underproducing hap1 mutations are alleles of the same gene which regulates in trans the expression of the structural genes encoding iso-cytochromes c

1986 ◽  
Vol 10 (5) ◽  
pp. 339-342 ◽  
Author(s):  
J. Verdi�re ◽  
F. Creusot ◽  
L. Guarente ◽  
P. P. Slonimski
Keyword(s):  
Structural Genes ◽  
Cytochromes C ◽  
Genes Encoding ◽  
In Trans

Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

scholarly journals A gene cluster involved in the biosynthesis of vanchrobactin, a chromosome-encoded siderophore produced by Vibrio anguillarum

Microbiology ◽  
2006 ◽  
Vol 152 (12) ◽  
pp. 3517-3528 ◽  
Author(s):  
Miguel Balado ◽  
Carlos R. Osorio ◽  
Manuel L. Lemos
Keyword(s):  
Gene Cluster ◽  
Vibrio Anguillarum ◽  
No Production ◽  
Genetic Determinants ◽  
Dihydroxybenzoic Acid ◽  
Peptide Synthetase ◽  
Genes Encoding ◽  
In Trans ◽  
Culture Supernatants

Vibrio anguillarum serotype O2 strains produce a catechol siderophore named vanchrobactin, which has been identified as N-[N′-(2,3-dihydroxybenzoyl)-arginyl]-serine. This work describes a chromosomal region that harbours the genetic determinants necessary for the biosynthesis of vanchrobactin. The authors have identified the genes involved in 2,3-dihydroxybenzoic acid (DHBA) biosynthesis (vabA, vabB and vabC) and activation (vabE), and a gene (vabF) encoding a non-ribosomal peptide synthetase, which is putatively involved in the assembly of the siderophore components. Also described are the identification and characterization of genes encoding a putative vanchrobactin exporter (vabS) and a siderophore esterase (vabH). In-frame deletion mutants in vabA, vabB, vabC, vabE, vabF and vabH were impaired for growth under conditions of iron limitation, and the analysis of culture supernatants by chrome azurol-S and cross-feeding assays showed almost no production of siderophores in any of the vabABCEF mutants. In addition, deletion mutations of vabA, vabB and vabC abolished production of DHBA, as assessed by chemical and biological analyses. Complementation of each mutant with the corresponding gene provided in trans confirmed the involvement of this gene cluster in the biosynthesis of DHBA and vanchrobactin in V. anguillarum strain RV22. Based on chemical and genetic data, and on published models for other catechol siderophores, a model for vanchrobactin biosynthesis is proposed.

Cloning and nucleotide sequence of the structural genes encoding the formate dehydrogenase of Wolinella succinogenes

1991 ◽  
Vol 156 (2) ◽  
pp. 119-128 ◽  
Author(s):  
M. Bokranz ◽  
M. Gutmann ◽  
C. Körtner ◽  
E. Kojro ◽  
F. Fahrenholz ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Formate Dehydrogenase ◽  
Wolinella Succinogenes ◽  
Structural Genes ◽  
Genes Encoding

Synthesis and expression of genes encoding tuna, pigeon, and horse cytochromes c in the yeast Saccharomyces cerevisiae

Gene ◽  
1991 ◽  
Vol 105 (1) ◽  
pp. 73-81 ◽  
Author(s):  
David R. Hickey ◽  
Krishna Jayaraman ◽  
Charles T. Goodhue ◽  
Janak Shah ◽  
Sarah A. Fingar ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Yeast Saccharomyces Cerevisiae ◽  
Expression Of Genes ◽  
Cytochromes C ◽  
Genes Encoding

scholarly journals Overproduction of Wild-Type and Bioengineered Derivatives of the Lantibiotic Lacticin 3147

2006 ◽  
Vol 72 (6) ◽  
pp. 4492-4496 ◽  
Author(s):  
Paul D. Cotter ◽  
Lorraine A. Draper ◽  
Elaine M. Lawton ◽  
Olivia McAuliffe ◽  
Colin Hill ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Structural Genes ◽  
Wild Type ◽  
Genetic Determinants ◽  
Lacticin 3147 ◽  
Genes Encoding ◽  
High Level ◽  
Derivatives Of

ABSTRACT Lacticin 3147 is a broad-spectrum two-peptide lantibiotic whose genetic determinants are located on two divergent operons on the lactococcal plasmid pMRC01. Here we introduce each of 14 subclones, containing different combinations of lacticin 3147 genes, into MG1363 (pMRC01) and determine that a number of them can facilitate overproduction of the lantibiotic. Based on these studies it is apparent that while the provision of additional copies of genes encoding the biosynthetic/production machinery and the regulator LtnR is a requirement for high-level overproduction, the presence of additional copies of the structural genes (i.e., ltnA1A2) is not.

scholarly journals Non-Coding RNAs in Bacteria

2018 ◽  
Author(s):  
Amanda Carvalho Garcia ◽  
Vera Lúcia Pereira dos Santos ◽  
Teresa Cristina Santos Cavalcanti ◽  
Luiz Martins Collaço ◽  
Iara José Taborda de Messias ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Physiological Responses ◽  
Coding Regions ◽  
Short Rnas ◽  
Genes Encoding ◽  
Intergenic Regions ◽  
In Trans ◽  
Rna Interaction ◽  
In Cis ◽  
Target Rna

Genes encoding regulatory RNAs known as short RNAs (sRNAs) or non-coding sRNAs (ncRNAs), modulate physiological responses through different mechanisms, through RNA-RNA interaction or RNA-protein interaction. These molecules transcribed in trans and in cis relative to the target RNA. They are located between the coding regions of proteins, i.e., in the intergenic regions of the genome and show signals of promoters and termini sequences generally Rho-independent. The size of the ncRNAs genes ranges from ~ 50 to ~ 500 nucleotides and several transcripts are processed by RNase with smaller end products, which modulate physiological responses through different mechanisms, by RNA-RNA interaction or RNA-protein interactions and some interactions may be stabilized by the Hfq chaperone. The Riboswitches constitute another class of ncRNAs, located in the 5'UTR region of an mRNA that promote transcriptional regulation through their interaction with a linker molecule. Recently, in prokaryotes, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) regions have described, which repeats of sequences of palindromic bases are. Each replicate consists of short segments of "spacer DNA" from exposures prior to a bacteriophage virus or exogenous plasmid. The CRISPR system consists of an immune system of resistance to exogenous molecules.

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