The structural genes encoding CO dehydrogenase subunits (cox L, M and S) in Pseudomonas carboxydovorans OM5 reside on plasmid pHCG3 and are, with the exception of Streptomyces thermoautotrophicus, conserved in carboxydotrophic bacteria

1992 ◽  
Vol 157 (3) ◽  
pp. 301-304 ◽  
Author(s):  
Iris Hugendieck ◽  
Ortwin Meyer
Keyword(s):  
Structural Genes ◽  
Co Dehydrogenase ◽  
Genes Encoding ◽  
Carboxydotrophic Bacteria ◽  
Pseudomonas Carboxydovorans

Homology and distribution of CO dehydrogenase structural genes in carboxydotrophic bacteria

1989 ◽  
Vol 152 (4) ◽  
pp. 335-341 ◽  
Author(s):  
M. Kraut ◽  
I. Hugendieck ◽  
S. Herwig ◽  
O. Meyer
Keyword(s):  
Structural Genes ◽  
Co Dehydrogenase ◽  
Carboxydotrophic Bacteria

Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

Cloning and nucleotide sequence of the structural genes encoding the formate dehydrogenase of Wolinella succinogenes

1991 ◽  
Vol 156 (2) ◽  
pp. 119-128 ◽  
Author(s):  
M. Bokranz ◽  
M. Gutmann ◽  
C. Körtner ◽  
E. Kojro ◽  
F. Fahrenholz ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Formate Dehydrogenase ◽  
Wolinella Succinogenes ◽  
Structural Genes ◽  
Genes Encoding

scholarly journals Overproduction of Wild-Type and Bioengineered Derivatives of the Lantibiotic Lacticin 3147

2006 ◽  
Vol 72 (6) ◽  
pp. 4492-4496 ◽  
Author(s):  
Paul D. Cotter ◽  
Lorraine A. Draper ◽  
Elaine M. Lawton ◽  
Olivia McAuliffe ◽  
Colin Hill ◽  
...  
Keyword(s):  
Broad Spectrum ◽  
Structural Genes ◽  
Wild Type ◽  
Genetic Determinants ◽  
Lacticin 3147 ◽  
Genes Encoding ◽  
High Level ◽  
Derivatives Of

ABSTRACT Lacticin 3147 is a broad-spectrum two-peptide lantibiotic whose genetic determinants are located on two divergent operons on the lactococcal plasmid pMRC01. Here we introduce each of 14 subclones, containing different combinations of lacticin 3147 genes, into MG1363 (pMRC01) and determine that a number of them can facilitate overproduction of the lantibiotic. Based on these studies it is apparent that while the provision of additional copies of genes encoding the biosynthetic/production machinery and the regulator LtnR is a requirement for high-level overproduction, the presence of additional copies of the structural genes (i.e., ltnA1A2) is not.

scholarly journals Analysis of the structural genes encoding M-factor in the fission yeast Schizosaccharomyces pombe: identification of a third gene, mfm3.

1994 ◽  
Vol 14 (6) ◽  
pp. 3895-3905 ◽  
Author(s):  
S Kjaerulff ◽  
J Davey ◽  
O Nielsen
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Fission Yeast ◽  
Mutational Analysis ◽  
M Cells ◽  
Gene Products ◽  
Structural Genes ◽  
Triple Mutant ◽  
Isolation And Characterization ◽  
Genes Encoding

We previously identified two genes, mfm1 and mfm2, with the potential to encode the M-factor mating pheromone of the fission yeast Schizosaccharomyces pombe (J. Davey, EMBO J. 11:951-960, 1992), but further analysis revealed that a mutant strain lacking both genes still produced active M-factor. Here we describe the isolation and characterization of a third M-factor gene, mfm3. A mutant lacking all three genes fails to produce M-factor, indicating that all functional M-factor genes now have been identified. The triple mutant exhibits an absolute mating defect in M cells, a defect that is not rescued by addition of exogenous M-factor. A mutational analysis reveals that all three mfm genes contribute to the production of M-factor. Their transcription is limited to M cells and requires the mat1-Mc and ste11 gene products. Each gene is induced when the cells are starved of nitrogen and further induced by a pheromone signal. Additionally, the signal transduction machinery associated with the pheromone response is required for transcription of the mfm genes in both stimulated and unstimulated cells.

Sign in / Sign up

Export Citation Format

Share Document