scholarly journals Growth of Candida famata and Trichosporon cutaneum on uric acid as the sole source of carbon and energy, a hitherto unknown property of yeasts

1983 ◽  
Vol 49 (4-5) ◽  
pp. 361-368 ◽  
Author(s):  
W. J. Middelhoven ◽  
Jolanda A. van den Brink ◽  
M. Veenhuis
Keyword(s):  
Uric Acid ◽  
Sole Source ◽  
Trichosporon Cutaneum ◽  
Candida Famata ◽  
Unknown Property

Yeast species utilizing uric acid, adenine,n-alkylamines or diamines as sole source of carbon and energy

1985 ◽  
Vol 51 (3) ◽  
pp. 289-301 ◽  
Author(s):  
Wouter J. Middelhoven ◽  
Heleen de Kievit ◽  
André L. Biesbroek
Keyword(s):  
Uric Acid ◽  
Yeast Species ◽  
Sole Source

The digestion of organic matter, nitrogen and amino acids in calves fed semi-purified diets containing urea, uric acid or soya-bean meal

1977 ◽  
Vol 89 (3) ◽  
pp. 699-710 ◽  
Author(s):  
G. J. L. Jacobs ◽  
Jane Leibholz
Keyword(s):  
Amino Acids ◽  
Organic Matter ◽  
Uric Acid ◽  
Dry Matter ◽  
Essential Amino Acids ◽  
Sole Source ◽  
Soya Bean ◽  
Protein Nitrogen ◽  
Bean Meal ◽  
Soya Bean Meal

SummarySemi-purified diets containing urea (diet A), uric acid (diet B) or soya-bean meal (diet C) as the sole source of nitrogen were fed to two Friesian bull calves fitted with re-entrant duodenal cannulae. Total collections of digesta leaving the abomasum were made over 24-h periods.The flow of organic matter to the duodenum expressed as a percentage of intake increased from 35·8% (diet A) and 40·6% (diet B) for the non-protein nitrogen diets to 58·3% for diet C. A greater proportion of the apparent digestion of organic matter occurred in the forestomachs of the calves when fed diets A or B than when they were fed diet C.The flow of nitrogen from the abomasum expressed as a percentage of intake showed a significant increase (P< 0·05) from 65·4% for diet A to 84·4% for diet B and 85·1% for diet C. When diets B and C were fed to the calves a greater proportion of the apparent digestion of nitrogen occurred in the hindgut than when they were fed diet A. The synthesis of microbial protein was 13·9 g and 13·0 g for every 100 g of organic matter digested in the stomach when the calves were fed diets B and C and only 10·9 g when the calves were fed diet A.A significantly (P< 0·05) greater proportion of dry matter of the digesta at the duodenum was composed of amino acids on diet C (19·5%) than diet A (16·1%) with the proportion of essential amino acids (especially threonine, lysine, histidine and arginine) also being greater. The amino acid composition of the digesta dry matter on diet B was intermediate (17·2%).From the data presented, it was predicted that cystine and histidine were the first limiting amino acids for growth when the calves were fed the non-protein nitrogen diets (A and B).

scholarly journals Identification of a Formate-Dependent Uric Acid Degradation Pathway inEscherichia coli

2019 ◽  
Vol 201 (11) ◽  
Author(s):  
Yumi Iwadate ◽  
Jun-ichi Kato
Keyword(s):  
Escherichia Coli ◽  
Uric Acid ◽  
Formate Dehydrogenase ◽  
Sole Source ◽  
Degradation Pathway ◽  
Anaerobic Conditions ◽  
Content Type ◽  
Purine Catabolism ◽  
E Coli ◽  
Acid Degradation

ABSTRACTPurine is a nitrogen-containing compound that is abundant in nature. In organisms that utilize purine as a nitrogen source, purine is converted to uric acid, which is then converted to allantoin. Allantoin is then converted to ammonia. InEscherichia coli, neither urate-degrading activity nor a gene encoding an enzyme homologous to the known urate-degrading enzymes had previously been found. Here, we demonstrate urate-degrading activity inE. coli. We first identifiedaegAas anE. coligene involved in oxidative stress tolerance. An examination of gene expression revealed that bothaegAand its paralogygfTare expressed under both microaerobic and anaerobic conditions. TheygfTgene is localized within a chromosomal gene cluster presumably involved in purine catabolism. Accordingly, the expression ofygfTincreased in the presence of exogenous uric acid, suggesting thatygfTis involved in urate degradation. Examination of the change of uric acid levels in the growth medium with time revealed urate-degrading activity under microaerobic and anaerobic conditions in the wild-type strain but not in theaegA ygfTdouble-deletion mutant. Furthermore, AegA- and YgfT-dependent urate-degrading activity was detected only in the presence of formate and formate dehydrogenase H. Collectively, these observations indicate the presence of urate-degrading activity inE. colithat is operational under microaerobic and anaerobic conditions. The activity requires formate, formate dehydrogenase H, and eitheraegAorygfT. We also identified other putative genes which are involved not only in formate-dependent but also in formate-independent urate degradation and may function in the regulation or cofactor synthesis in purine catabolism.IMPORTANCEThe metabolic pathway of uric acid degradation to date has been elucidated only in aerobic environments and is not understood in anaerobic and microaerobic environments. In the current study, we showed thatEscherichia coli, a facultative anaerobic organism, uses uric acid as a sole source of nitrogen under anaerobic and microaerobic conditions. We also showed that formate, formate dehydrogenase H, and either AegA or YgfT are involved in uric acid degradation. We propose that formate may act as an electron donor for a uric acid-degrading enzyme in this bacterium.

391 Uric acid blood levels are associated with clinical and echocardiographic parameters of chronic heart failure

2003 ◽  
Vol 2 (1) ◽  
pp. 79
Author(s):  
P PAVLIDIS ◽  
J PARISSIS ◽  
S ANTONOPOULOS ◽  
D POLLATOS ◽  
P KIRIAZOPOULOS ◽  
...  
Keyword(s):  
Heart Failure ◽  
Uric Acid ◽  
Chronic Heart Failure ◽  
Blood Levels ◽  
Echocardiographic Parameters

Serum uric acid and obesity in Hawaiians

JAMA ◽  
1966 ◽  
Vol 196 (4) ◽  
pp. 364-365 ◽  
Author(s):  
L. A. Healey
Keyword(s):  
Uric Acid ◽  
Serum Uric Acid

Increased monocyte to HDL cholesterol ratio in vitamin B12 deficiency: is it related to cardiometabolic risk?

2020 ◽  
pp. 1-8
Author(s):  
Sanem Kayhan ◽  
Nazli Gulsoy Kirnap ◽  
Mercan Tastemur
Keyword(s):  
Risk Factors ◽  
Blood Pressure ◽  
Uric Acid ◽  
Vitamin B12 ◽  
Cardiometabolic Risk ◽  
Vitamin B12 Deficiency ◽  
Left Ventricular ◽  
Cardiometabolic Risk Factors ◽  
B12 Deficiency ◽  
Deficiency Group

Abstract. Vitamin B12 deficiency may have indirect cardiovascular effects in addition to hematological and neuropsychiatric symptoms. It was shown that the monocyte count-to-high density lipoprotein cholesterol (HDL-C) ratio (MHR) is a novel cardiovascular marker. In this study, the aim was to evaluate whether MHR was high in patients with vitamin B12 deficiency and its relationship with cardiometabolic risk factors. The study included 128 patients diagnosed with vitamin B12 deficiency and 93 healthy controls. Patients with vitamin B12 deficiency had significantly higher systolic blood pressure (SBP), diastolic blood pressure (DBP), MHR, C-reactive protein (CRP) and uric acid levels compared with the controls (median 139 vs 115 mmHg, p < 0.001; 80 vs 70 mmHg, p < 0.001; 14.2 vs 9.5, p < 0.001; 10.2 vs 4 mg/dl p < 0.001; 6.68 vs 4.8 mg/dl, p < 0.001 respectively). The prevalence of left ventricular hypertrophy was higher in vitamin B12 deficiency group (43.8%) than the control group (8.6%) (p < 0.001). In vitamin B12 deficiency group, a positive correlation was detected between MHR and SBP, CRP and uric acid (p < 0.001 r:0.34, p < 0.001 r:0.30, p < 0.001 r:0.5, respectively) and a significant negative correlation was detected between MHR and T-CHOL, LDL, HDL and B12 (p < 0.001 r: −0.39, p < 0.001 r: −0.34, p < 0.001 r: −0.57, p < 0.04 r: −0.17, respectively). MHR was high in vitamin B12 deficiency group, and correlated with the cardiometabolic risk factors in this group, which were SBP, CRP, uric acid and HDL. In conclusion, MRH, which can be easily calculated in clinical practice, can be a useful marker to assess cardiovascular risk in patients with vitamin B12 deficiency.

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