Trichosporon adeninovorans sp. nov., a yeast species utilizing adenine, xanthine, uric acid, putrescine and primary n-alkylamines as the sole source of carbon, nitrogen and energy

1984 ◽  
Vol 50 (4) ◽  
pp. 369-378 ◽  
Author(s):  
W. J. Middelhoven ◽  
M. C. Hoogkamer-Te Niet ◽  
N. J. W. Kreger-Van Rij
Keyword(s):  
Uric Acid ◽  
Yeast Species ◽  
Sole Source

Yeast species utilizing uric acid, adenine,n-alkylamines or diamines as sole source of carbon and energy

1985 ◽  
Vol 51 (3) ◽  
pp. 289-301 ◽  
Author(s):  
Wouter J. Middelhoven ◽  
Heleen de Kievit ◽  
André L. Biesbroek
Keyword(s):  
Uric Acid ◽  
Yeast Species ◽  
Sole Source

Trichosporon guehoae sp.nov., an anamorphic basidiomycetous yeast

1999 ◽  
Vol 45 (8) ◽  
pp. 686-690 ◽  
Author(s):  
Wouter J Middelhoven ◽  
Gloria Scorzetti ◽  
Jack W Fell
Keyword(s):  
Aromatic Compounds ◽  
Yeast Species ◽  
Large Subunit ◽  
Sole Source ◽  
Basidiomycetous Yeast ◽  
Phylogenetic Position ◽  
Base Sequencing

A morphological and physiological description of an anamorphic basidiomycetous yeast species, named Trichosporon guehoae (CBS 8521T), is presented. The ability to assimilate several aliphatic and aromatic compounds as sole source of carbon and energy is reported. The phylogenetic position within the genus, based on nuclear base sequencing of the D1/D2 region of the large subunit of rDNA is discussed.Key words: basidiomycetes, taxonomy, Trichosporon guehoae sp.nov., yeasts.

Nutrition and phylogeny of predacious yeasts

2000 ◽  
Vol 46 (6) ◽  
pp. 495-505 ◽  
Author(s):  
Marc-André Lachance ◽  
Ana Pupovac-Velikonja ◽  
Sabrina Natarajan ◽  
Birgit Schlag-Edler
Keyword(s):  
Candida Species ◽  
Yeast Species ◽  
Sole Source ◽  
Ammonium Nitrogen ◽  
Sulfate Transport ◽  
Prototheca Zopfii ◽  
Organic Sulfur Compounds ◽  
Sequence Relatedness ◽  
Ascomycetous Yeasts ◽  
Predacious Species

Yeast predation was studied with respect to the range of its distribution among ascomycetous yeasts, the range of yeast species that can be affected, and nutritional aspects of the phenomenon. The yeasts identified as predators belong to the Saccharomycopsis clade as defined on the basis of rDNA sequence relatedness. The 11 recognized species in the clade, plus three undescribed but related Candida species, were shown to be incapable of utilizing sulfate as sole source of sulfur, and all but two (Saccharomycopsis capsularis andSaccharomycopsis vini) were observed to penetrate and kill other yeasts under some conditions. Other unrelated sulfate transport-deficient yeasts (strains in the genera Pichia and Candida and the two known species of Starmera) are not predacious. The predacious species vary considerably as to the optimal environmental conditions that favour predation. Some are inhibited by the presence of rich nitrogenous nutrients, organic sulfur compounds, or higher concentrations of ammonium nitrogen, whereas other species may be stimulated under the same conditions. An attempt was made to correlate prey susceptibility to the excretion of substances that stimulate the growth of predators, but no correlation was detected between the two phenomena. The range of susceptible prey covers both ascomycetes and basidiomycetes, and includes Schizosaccharomyces pombe, which was previously thought to be immune. The achlorophyllous alga Prototheca zopfii is not killed by predacious yeasts, but the initial steps of penetration have been observed in some cases. Predacious species attack other predacious species, and in some cases, young cultures may penetrate older cultures of the same strain.Key words: predacious yeasts, sulfate transport deficiency, Saccharomycopsis.

Cryptococcus tephrensis, sp.nov., and Cryptococcus heimaeyensis, sp.nov.; new anamorphic basidiomycetous yeast species from Iceland

2002 ◽  
Vol 48 (5) ◽  
pp. 463-467 ◽  
Author(s):  
Helen S Vishniac
Keyword(s):  
New Species ◽  
Yeast Species ◽  
Sequence Data ◽  
Growth Failure ◽  
Nitrogen Sources ◽  
Sole Source ◽  
Lsu Rdna ◽  
Two New Species ◽  
Physiological Profiles ◽  
Maximum Temperatures

Two new species from Iceland are described on the basis of physiological profiles and sequence data from the D2 region of LSU rDNA: Cryptococcus tephrensis (type ICE99-1ToM Y5, ATCC MYA-1765, CBS 8935, GenBank AF317208) and Cryptococcus heimaeyensis (type ICE99-1ToM Y8, ATCC MYA-1759, CBS 8933, GenBank AF370717). The two new species are identifiable from sequence data and can be distinguished from their closest relative, Cryptococcus victoriae, by their higher maximum temperatures for growth, failure to utilize nitrate as sole nitrogen source, and utilization of cadaverine and ethylamine as sole nitrogen sources. Cryptococcus tephrensis is distinguishable from C. heimaeyensis by failure to grow on saccharate as sole source of carbon and energy.Key words: Iceland, Cryptococcus, soil yeasts.

The digestion of organic matter, nitrogen and amino acids in calves fed semi-purified diets containing urea, uric acid or soya-bean meal

1977 ◽  
Vol 89 (3) ◽  
pp. 699-710 ◽  
Author(s):  
G. J. L. Jacobs ◽  
Jane Leibholz
Keyword(s):  
Amino Acids ◽  
Organic Matter ◽  
Uric Acid ◽  
Dry Matter ◽  
Essential Amino Acids ◽  
Sole Source ◽  
Soya Bean ◽  
Protein Nitrogen ◽  
Bean Meal ◽  
Soya Bean Meal

SummarySemi-purified diets containing urea (diet A), uric acid (diet B) or soya-bean meal (diet C) as the sole source of nitrogen were fed to two Friesian bull calves fitted with re-entrant duodenal cannulae. Total collections of digesta leaving the abomasum were made over 24-h periods.The flow of organic matter to the duodenum expressed as a percentage of intake increased from 35·8% (diet A) and 40·6% (diet B) for the non-protein nitrogen diets to 58·3% for diet C. A greater proportion of the apparent digestion of organic matter occurred in the forestomachs of the calves when fed diets A or B than when they were fed diet C.The flow of nitrogen from the abomasum expressed as a percentage of intake showed a significant increase (P< 0·05) from 65·4% for diet A to 84·4% for diet B and 85·1% for diet C. When diets B and C were fed to the calves a greater proportion of the apparent digestion of nitrogen occurred in the hindgut than when they were fed diet A. The synthesis of microbial protein was 13·9 g and 13·0 g for every 100 g of organic matter digested in the stomach when the calves were fed diets B and C and only 10·9 g when the calves were fed diet A.A significantly (P< 0·05) greater proportion of dry matter of the digesta at the duodenum was composed of amino acids on diet C (19·5%) than diet A (16·1%) with the proportion of essential amino acids (especially threonine, lysine, histidine and arginine) also being greater. The amino acid composition of the digesta dry matter on diet B was intermediate (17·2%).From the data presented, it was predicted that cystine and histidine were the first limiting amino acids for growth when the calves were fed the non-protein nitrogen diets (A and B).

scholarly journals Identification of a Formate-Dependent Uric Acid Degradation Pathway inEscherichia coli

2019 ◽  
Vol 201 (11) ◽  
Author(s):  
Yumi Iwadate ◽  
Jun-ichi Kato
Keyword(s):  
Escherichia Coli ◽  
Uric Acid ◽  
Formate Dehydrogenase ◽  
Sole Source ◽  
Degradation Pathway ◽  
Anaerobic Conditions ◽  
Content Type ◽  
Purine Catabolism ◽  
E Coli ◽  
Acid Degradation

ABSTRACTPurine is a nitrogen-containing compound that is abundant in nature. In organisms that utilize purine as a nitrogen source, purine is converted to uric acid, which is then converted to allantoin. Allantoin is then converted to ammonia. InEscherichia coli, neither urate-degrading activity nor a gene encoding an enzyme homologous to the known urate-degrading enzymes had previously been found. Here, we demonstrate urate-degrading activity inE. coli. We first identifiedaegAas anE. coligene involved in oxidative stress tolerance. An examination of gene expression revealed that bothaegAand its paralogygfTare expressed under both microaerobic and anaerobic conditions. TheygfTgene is localized within a chromosomal gene cluster presumably involved in purine catabolism. Accordingly, the expression ofygfTincreased in the presence of exogenous uric acid, suggesting thatygfTis involved in urate degradation. Examination of the change of uric acid levels in the growth medium with time revealed urate-degrading activity under microaerobic and anaerobic conditions in the wild-type strain but not in theaegA ygfTdouble-deletion mutant. Furthermore, AegA- and YgfT-dependent urate-degrading activity was detected only in the presence of formate and formate dehydrogenase H. Collectively, these observations indicate the presence of urate-degrading activity inE. colithat is operational under microaerobic and anaerobic conditions. The activity requires formate, formate dehydrogenase H, and eitheraegAorygfT. We also identified other putative genes which are involved not only in formate-dependent but also in formate-independent urate degradation and may function in the regulation or cofactor synthesis in purine catabolism.IMPORTANCEThe metabolic pathway of uric acid degradation to date has been elucidated only in aerobic environments and is not understood in anaerobic and microaerobic environments. In the current study, we showed thatEscherichia coli, a facultative anaerobic organism, uses uric acid as a sole source of nitrogen under anaerobic and microaerobic conditions. We also showed that formate, formate dehydrogenase H, and either AegA or YgfT are involved in uric acid degradation. We propose that formate may act as an electron donor for a uric acid-degrading enzyme in this bacterium.

391 Uric acid blood levels are associated with clinical and echocardiographic parameters of chronic heart failure

2003 ◽  
Vol 2 (1) ◽  
pp. 79
Author(s):  
P PAVLIDIS ◽  
J PARISSIS ◽  
S ANTONOPOULOS ◽  
D POLLATOS ◽  
P KIRIAZOPOULOS ◽  
...  
Keyword(s):  
Heart Failure ◽  
Uric Acid ◽  
Chronic Heart Failure ◽  
Blood Levels ◽  
Echocardiographic Parameters
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