Selective enrichment for genetic markers in DNA released by competent cultures of Bacillus subtilis

1977 ◽  
Vol 155 (2) ◽  
pp. 179-183 ◽  
Author(s):  
W. Douglas Crabb ◽  
Uldis N. Streips ◽  
R. J. Doyle
Keyword(s):  
Bacillus Subtilis ◽  
Genetic Markers ◽  
Selective Enrichment

Malachite green bioremoval by a newly isolated strain Citrobacter sedlakii RI11; enhancement of the treatment by biosurfactant addition

2015 ◽  
Vol 72 (8) ◽  
pp. 1283-1293 ◽  
Author(s):  
Inès Mnif ◽  
Raouia Fendri ◽  
Dhouha Ghribi
Keyword(s):  
Bacillus Subtilis ◽  
Effective Treatment ◽  
Malachite Green ◽  
Ph Value ◽  
Synthetic Dyes ◽  
Alkaline Ph ◽  
Selective Enrichment ◽  
Decolorization Efficiency ◽  
Higher Doses ◽  
Microbial Surfactant

Citrobacter sedlackii RI11, isolated from acclimated textile effluent after selective enrichment on synthetic dyes, was assessed for malachite green (MG) biotreatment potency. Results indicate that this bacterium has potential for use in effective treatment of MG contaminated wastewaters under shaking conditions at neutral and alkaline pH value, characteristic of typical textile effluents. Also, the newly isolated strain can tolerate higher doses of dye and decolorize up to 1,000 mg/l of dye. When used as microbial surfactant to enhance MG biodecolorization, Bacillus subtilis SPB1-derived lipopeptide accelerated the decolorization rate and maximized the decolorization efficiency at an optimal concentration of biosurfactant of about 0.075%. Studies ensured that MG removal by this strain could be due to biodegradation and/or adsorption. Results on germination potencies of different seeds using the treated dyes under different conditions favor the use of SPB1 biosurfactant for the treatment of MG.

scholarly journals Cotransduction and Cotransformation of Genetic Markers in Bacillus subtilis and Bacillus licheniformis

1969 ◽  
Vol 100 (2) ◽  
pp. 1027-1036 ◽  
Author(s):  
Franklin J. Tyeryar ◽  
Martha J. Taylor ◽  
William D. Lawton ◽  
Ivan D. Goldberg
Keyword(s):  
Bacillus Subtilis ◽  
Genetic Markers ◽  
Bacillus Licheniformis

scholarly journals CONGRESSION OF UNLINKED MARKERS AND GENETIC MAPPING IN THE TRANSFORMATION OF BACILLUS SUBTILIS 168

Genetics ◽  
1973 ◽  
Vol 73 (1) ◽  
pp. 13-21
Author(s):  
Robert J Erickson ◽  
James C Copeland
Keyword(s):  
Bacillus Subtilis ◽  
Genetic Mapping ◽  
Genetic Markers ◽  
Competent Cell ◽  
Dna Molecules ◽  
Bacillus Subtilis 168

ABSTRACT A thorough examination of cotransformation of two unlinked genetic markers in Bacillus subtilis 168 shows that the two recombinational events do not occur randomly. The cotransformation frequency is dependent on the distance between the two markers as well as on the order in which they replicate in the competent cell. These results indicate that uptake and/or integration of DNA molecules bearing these genetic markers is enhanced at the time these markers replicate in the competent cell.

scholarly journals 211 Evaluation of CLOSTAT-500® on growth performance and fecal Salmonella prevalence in yearling beef steers during the feedlot receiving phase

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 155-155
Author(s):  
Zachary K Smith ◽  
Paul Rand Broadway ◽  
Nicole C Burdick Sanchez ◽  
Jeff A Carroll ◽  
Doug Lafleur ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Growth Performance ◽  
Block Design ◽  
Experimental Unit ◽  
Management Approach ◽  
Beef Steers ◽  
Fecal Samples ◽  
Selective Enrichment ◽  
Des Moines ◽  
Rectal Palpation

Abstract Two-hundred and thirty eight crossbred beef steers (initial BW = 419 ± 32.4 kg) were used to evaluate the influence of a patented probiotic on growth performance responses and fecal Salmonella prevalence during a 28-d feedlot receiving period at the South East Research Farm (SERF) feedlot located near Beresford, SD. Steers were allotted to 24 pens (9 to 10 steers/pen) and assigned to one of two treatments (12 pens/treatment): no probiotic (CON) or 0.5 g·steer-1·d-1 of a Bacillus subtilis PB6 probiotic (CLOSTAT-500®, Kemin Industries, Des Moines, IA; CLO). Steers were transitioned from a 70% concentrate diet (DM basis) to a 92% concentrate diet (DM basis) over a 14-d period. The final diet contained (DM basis): 12.5% CP, 2.08 Mcal/kg of NEm, 1.40 Mcal/kg of NEg, 30 g/907-kg of monensin sodium, and 0.45 mg/kg of chromium propionate (KemTRACE® Chromium, Kemin Industries, Des Moines, IA). Fecal samples were collected on study d 1 and 28 (6 and 34 d following arrival to the SERF, respectively) from sentinel steers (n = 5/pen) via rectal palpation and composited by pen for determination of Salmonella prevalence using selective enrichment and culture medias. Steers were fed once daily and bunks were managed according to a slick bunk management approach. Data were analyzed as a randomized complete block design; pen served as the experimental unit and an α of 0.05 determined significance. No differences were detected (P ≥ 0.25) between treatments for ADG, DMI, or gain efficiency during the initial 28-d feedlot receiving phase. Additionally, no Salmonella was recovered in any fecal samples collected. These data indicate that Bacillus subtilis PB6 had no influence on receiving phase growth performance, and fecal Salmonella prevalence was not observed in yearling steers placed on feed in March in southeastern South Dakota.

scholarly journals Identification and Characterization of Novel Genetic Markers Associated with Biological Control Activities in Bacillus subtilis

2006 ◽  
Vol 96 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Raghavendra Joshi ◽  
Brian B. McSpadden Gardener
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Genetic Markers ◽  
Plant Pathogens ◽  
Plant Growth Promoting Rhizobacteria ◽  
Subtractive Hybridization ◽  
Pythium Ultimum ◽  
Suppressive Subtractive Hybridization ◽  
Antibiotic Biosynthesis ◽  
Wide Range

Suppressive subtractive hybridization (SSH) was used to identify genetic markers associated with biological control of plant pathogens by Bacillus subtilis. The genomes of two commercialized strains, GB03 and QST713, were compared with that of strain 168, which has no defined biocontrol capacities, to obtain a pool of DNA fragments unique to the two biocontrol strains. The sequences of 149 subtracted fragments were determined and compared with those present in GenBank, but only 80 were found to correspond to known Bacillus genes. Of these, 65 were similar to genes with a wide range of metabolic functions, including the biosynthesis of cell wall components, sporulation, and antibiotic biosynthesis. Sixteen subtracted fragments shared a high degree of similarity to sequences found in multiple B. subtilis strains with proven biocontrol capacities. Oligonucleotide primers specific to nine of these genes were developed. The targeted genes included five genes involved in antibiotic synthesis (bmyB, fenD, ituC,srfAA, and srfAB) and four additional genes (yndJ, yngG, bioA, and a hypothetical open reading frame) not previously associated with biological control. All nine markers were amplified from the commercialized B. subtilis strains GB03, QST713, and MBI600, with the exception of ituC, which was not detected in GB03. The markers also were amplified from four other B. subtilis isolates, but they were not amplified from other related Bacillus strains, including the plant growth-promoting rhizobacteria IN937a and IN937b. Sequencing of the amplified markers revealed that all seven of the isolates that scored positive for multiple markers were genotypically distinct strains. Interestingly, strains scored positive for the amplifiable markers generally were more effective at inhibiting the growth of Rhizoctonia solani and Pythium ultimum than other Bacillus isolates that lacked the markers. The potential utility of the defined genetic markers to further define the diversity, ecology, and biocontrol activities of B. subtilis are discussed.

Sign in / Sign up

Export Citation Format

Share Document