Malachite green bioremoval by a newly isolated strain Citrobacter sedlakii RI11; enhancement of the treatment by biosurfactant addition

2015 ◽  
Vol 72 (8) ◽  
pp. 1283-1293 ◽  
Author(s):  
Inès Mnif ◽  
Raouia Fendri ◽  
Dhouha Ghribi
Keyword(s):  
Bacillus Subtilis ◽  
Effective Treatment ◽  
Malachite Green ◽  
Ph Value ◽  
Synthetic Dyes ◽  
Alkaline Ph ◽  
Selective Enrichment ◽  
Decolorization Efficiency ◽  
Higher Doses ◽  
Microbial Surfactant

Citrobacter sedlackii RI11, isolated from acclimated textile effluent after selective enrichment on synthetic dyes, was assessed for malachite green (MG) biotreatment potency. Results indicate that this bacterium has potential for use in effective treatment of MG contaminated wastewaters under shaking conditions at neutral and alkaline pH value, characteristic of typical textile effluents. Also, the newly isolated strain can tolerate higher doses of dye and decolorize up to 1,000 mg/l of dye. When used as microbial surfactant to enhance MG biodecolorization, Bacillus subtilis SPB1-derived lipopeptide accelerated the decolorization rate and maximized the decolorization efficiency at an optimal concentration of biosurfactant of about 0.075%. Studies ensured that MG removal by this strain could be due to biodegradation and/or adsorption. Results on germination potencies of different seeds using the treated dyes under different conditions favor the use of SPB1 biosurfactant for the treatment of MG.

Biosorption of Congo Red from aqueous solution by Bacillus weihenstephanensis RI12; effect of SPB1 biosurfactant addition on biodecolorization potency

2015 ◽  
Vol 72 (6) ◽  
pp. 865-874 ◽  
Author(s):  
Inès Mnif ◽  
Raouia Fendri ◽  
Dhouha Ghribi
Keyword(s):  
Congo Red ◽  
Azo Dye ◽  
Ph Value ◽  
Energy Costs ◽  
Time Saving ◽  
Bacillus Weihenstephanensis ◽  
Decolorization Efficiency ◽  
Higher Doses ◽  
Adsorption Phenomena ◽  
Microbial Surfactant

Bacillus weihenstephanensis RI12, isolated from hydrocarbon contaminated soil, was assessed for Congo Red bio-treatment potency. Results suggested the potential of this bacterium for use in effective treatment of Congo Red contaminated wastewaters under shaking conditions at acidic and neutral pH value. The strain could tolerate higher doses of dyes as it could decolorize up to 1,000 mg/l of Congo Red. When used as microbial surfactant to enhance Congo Red biodecolorization, Bacillus subtilis SPB1-derived lipopeptide accelerated the decolorization rate and maximized the decolorization efficiency at an optimal concentration of biosurfactant of about 0.075%. Studies ensured that Congo Red removal by this strain could be due to an adsorption phenomena. Germination potencies of tomato seeds using the treated dyes under different conditions showed the efficient biotreatment of the azo dye Congo Red especially with the addition of SPB1 biosurfactant. To conclude, the addition of SPB1 bioemulsifier reduced energy costs by reducing the effective decolorization period; the biosurfactant stimulated bacterial decolorization method may provide a highly efficient, inexpensive and time-saving procedure in the treatment of textile effluents.

scholarly journals Composition, stability and stereo effects of zirconium(IV) dl-tartrate formation

2019 ◽  
Vol 57 (2) ◽  
pp. 28-34
Author(s):  
Lilia I. Mukhamedyarova ◽  
◽  
Sergey G. Bezryadin ◽  
Elena Yu. Klukvina ◽  
Vladimir V. Chevela ◽  
...  
Keyword(s):  
Tartaric Acid ◽  
Ph Value ◽  
Acid System ◽  
Alkaline Ph ◽  
Titration Method ◽  
Software Complex ◽  
Tartaric Acids ◽  
Potentiometric Titration Method ◽  
High Concentrations ◽  
Metal Ligand

The system of zirconium (IV) – dl-tartaric acid for metal: ligand 1: 1, 1: 2 and 1: 3 ratios in aqueous solution has been studied by means of using potentiometric titration method in combination with mathematical modeling. The comparison of Bjerrum functions from pH for zirconium(IV) systems: d-tartaric acid and zirconium (IV): dl-tartaric acid, has revealed the following features in the behavior of the curves: the degree of titration for the complexes at a fixed pH value for systems with dl-tartaric acid is more than for d-acid. The CPESSP software complex has calculated the composition, stability constants and molar fractions of zirconium(IV) tartrate accumulation. It has been also found that at a ratio of 1: 1 for Zr (IV) and ligand (H4Tart) ions in the system under study ZrHTart+ is formed, which is tetramerized into Zr4Tart40 and, further, tetranuclear particles of varying degrees of deprotonization are formed, as well as mononuclear forms. In a strongly alkaline pH environment > 10, Bjerrum curves for d- and dl-tartaric acids overlap each other and correspond to hydroxocomplexes of varying degrees of titration. For the 1: 2 ratio, the composition of the complexes for the zirconium(IV) – dl-H4T system is slightly different; compared to the zirconium(IV) – dH4T system, differences are clearly observed for both low and high concentrations. Based on these data, a complex formation scheme in the Zr(IV) – dl-tartaric acid system has been proposed for all the ratios studied. The characteristics of stereoselective diastereomer formation have been calculated. It has been revealed that in the medium of racemic tartrate, ddd- and lll-Zr(H2Tart)2(HTart)3-forms, as well as Zr(H2Tart)(НTart)24-Zr(HTart)35- are formed on a stereoselective basis.

scholarly journals Photodegradation of fluoroquinolones in aqueous solution under light conditions relevant to surface waters, toxicity assessment of photoproduct mixtures.

2021 ◽  
Author(s):  
Sarka Klementova ◽  
Martina Poncarová ◽  
Helena Langhansová ◽  
Jaroslava Lieskovská ◽  
David Kahoun ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Antibacterial Activity ◽  
Surface Waters ◽  
Ph Value ◽  
Reaction System ◽  
Photochemical Degradation ◽  
Alkaline Ph ◽  
Light Conditions ◽  
E Coli ◽  
Neutral Ph

Abstract Photochemical degradation of fluoroquinolones ciprofloxacin, enrofloxacin and norfloxacin in aqueous solution under light conditions relevant to surface waters at neutral and alkaline pH was found to proceed readily with half-lives between 0.9 and 2.7 min. The products of photochemical degradation identified by HPLC-MS included defluorinated, hydroxylated, and decarboxylated structures as well as structures with opened cyclic structures. For all of the studied substances, the reaction pathways were influenced significantly by the pH of the reaction system, with more products formed at alkaline pH than at neutral pH: the ratios of products in neutral and alkaline pH were 16/26, 9/19, 15/23 for ciprofloxacin, enrofloxacin, and norfloxacin, respectively. The structures of photoproducts and pathways of photochemical degradation are proposed. The antibacterial activities of photoproduct mixtures tested on E. coli and S. epidermidis were significantly higher in comparison to parental antibiotics in the case of both ciprofloxacin and enrofloxacin with p-values less than 0.0001 in most cases. The effect of the photoproducts was shown to be dependent on the pH value of the original antibiotic solutions before photodegradation: for ciprofloxacin, antibacterial activity against E. coli was more notably pronounced with regard to neutral pH photoproducts, while a less significant, or in one case not significant, effect of pH was observed against S. epidermidis ; for norfloxacin, antibacterial activity against both E. coli and S. epidermidis were especially high with regard to alkaline pH photoproducts

Application of Immobilized Laccase from Bacillus subtilis MTCC 2414 on Decolourization of Synthetic Dyes

2015 ◽  
Vol 10 (9) ◽  
pp. 421-432 ◽  
Author(s):  
S. Murugan ◽  
P.M. Narayanan ◽  
A.S. Eva ◽  
S.U. Devina ◽  
S. Kalidass
Keyword(s):  
Bacillus Subtilis ◽  
Synthetic Dyes ◽  
Immobilized Laccase

Selective enrichment for genetic markers in DNA released by competent cultures of Bacillus subtilis

1977 ◽  
Vol 155 (2) ◽  
pp. 179-183 ◽  
Author(s):  
W. Douglas Crabb ◽  
Uldis N. Streips ◽  
R. J. Doyle
Keyword(s):  
Bacillus Subtilis ◽  
Genetic Markers ◽  
Selective Enrichment

scholarly journals PENGARUH LAMA FERMENTASI OLEH Bacillus subtilis TERHADAP KARAKTERISTIK SERE KEDELE

2019 ◽  
Vol 8 (3) ◽  
pp. 226
Author(s):  
Sri Madiarti Sipayung ◽  
I Wayan Rai Widarta ◽  
I Desak Putu Kartika Pratiwi
Keyword(s):  
Bacillus Subtilis ◽  
Ph Value ◽  
Total Acid ◽  
Fermentation Time ◽  
Crude Fiber Content ◽  
Randomized Design ◽  
Result Show ◽  
Soybean Fermentation ◽  
Completely Randomized Design ◽  
Normal Taste

This research aims to find out the effect of soybean fermentation time by Bacillus subtilis to produce sere kedele with the best characteristics. This research used completely randomized design (CRD) with one treatment factor of the fermentation time with 5 period of fermentation time (12, 18, 24, 30 and 36h). Each treatment was repeated 3 times, resulted 15 experimental units. The data were then analyzed with Analysis of Variance method and if the treatment had an effect on the variable, followed by the Duncan test. The result show that fermentation times had significant effect to moisture, ash, protein, fat, carbohydrate, crude fiber content, total acid, pH value, the number microbes, hedonic test for taste, scoring test for taste and overall acceptance of sere kedele. Fermented by B. subtilis for 12h resulted in the best characteristic under the following criteria : 58.05% moisture content, 2.39% ash content, 15.79% protein content, 14.39% fat contetnt, 9,38% carbohydrate content, 6.1 pH value, 0.11% total acid, 9.59log cfu/g the number microbes, color, flavour, texture liked, normal taste with sour taste and overall acceptance liked.   Keyword : soybean, sere kedele, Bacillus subtilis, fermentation, traditional food .

A β-Mannanase from Bacillus subtilis B36: Purification, Properties, Sequencing, Gene Cloning and Expression in Escherichia coli

2006 ◽  
Vol 61 (11-12) ◽  
pp. 840-846 ◽  
Author(s):  
Ya Nan Li ◽  
Kun Meng ◽  
Ya Ru Wang ◽  
Bin Yao
Keyword(s):  
Escherichia Coli ◽  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Specific Activity ◽  
Ph Value ◽  
High Specificity ◽  
Apparent Molecular Weight ◽  
Cloning And Expression ◽  
Gene Cloning And Expression ◽  
Reading Frame

Abstract MANB36, a secrete endo-β-1,4-D-mannanase produced by Bacillus subtilis B36, was puri­fied to homogeneity from a culture supernatant and characterized. The optimum pH value for the mannanase activity of MANB36 is 6.4 and the optimum temperature is 50 °C. The enzyme activity of MANB36 is remarkably thermostable at 60 °C and the specific activity of MANB36 is 927.84 U/mg. Metal cations (except Hg2+ and Ag+), EDTA and 2-mercaptoetha- nol (2-ME) have no effects on enzyme activity. This enzyme exhibits high specificity with the substituted galactomannan locust bean gum (LBG). The gene encoding for MANB36, manB36, was cloned by PCR and sequenced. manB36 contains a single open reading frame (ORF) consisting of 1104 bp that encodes a protein of 367 amino acids. The predicted mo­lecular weight of 38.13 kDa, calculated by the deduced protein of the gene manB36 without signal peptide, coincides with the apparent molecular weight of 38.0 kDa of the purified MANB36 estimated by SDS-PAGE. The mature protein of MANB36 has been expressed in Escherichia coli BL21 and the expressed mannanase has normal bioactivity.

scholarly journals Photodegradation of Malachite Green by Nanostructured BiWO Visible Light-Induced Photocatalyst

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Yijie Chen ◽  
Yaqin Zhang ◽  
Chen Liu ◽  
Aimin Lu ◽  
Weihua Zhang
Keyword(s):  
Visible Light ◽  
Malachite Green ◽  
Ph Value ◽  
Photocatalytic Efficiency

Bi2WO6photocatalyst was first utilized to degrade malachite green. The effects of the concentration of malachite green, the pH value, and the concentration of Bi2WO6on the photocatalytic efficiency were investigated. This study presents a strategy to eliminate highly toxic and persistent dyes such as malachite green.

scholarly journals Effective treatment for suppression of acrylamide formation in fried potato chips using L-asparaginase from Bacillus subtilis

3 Biotech ◽  
2015 ◽  
Vol 5 (5) ◽  
pp. 783-789 ◽  
Author(s):  
Yohei Onishi ◽  
Asep A. Prihanto ◽  
Shigekazu Yano ◽  
Kazuyoshi Takagi ◽  
Midori Umekawa ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Effective Treatment ◽  
Potato Chips ◽  
Acrylamide Formation
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