Physical and mapping properties of distant linkages between genetic markers in transformation of Bacillus subtilis

1967 ◽  
Vol 99 (4) ◽  
pp. 333-349 ◽  
Author(s):  
M. S. Kelly
Keyword(s):  
Bacillus Subtilis ◽  
Genetic Markers

Selective enrichment for genetic markers in DNA released by competent cultures of Bacillus subtilis

1977 ◽  
Vol 155 (2) ◽  
pp. 179-183 ◽  
Author(s):  
W. Douglas Crabb ◽  
Uldis N. Streips ◽  
R. J. Doyle
Keyword(s):  
Bacillus Subtilis ◽  
Genetic Markers ◽  
Selective Enrichment

scholarly journals Cotransduction and Cotransformation of Genetic Markers in Bacillus subtilis and Bacillus licheniformis

1969 ◽  
Vol 100 (2) ◽  
pp. 1027-1036 ◽  
Author(s):  
Franklin J. Tyeryar ◽  
Martha J. Taylor ◽  
William D. Lawton ◽  
Ivan D. Goldberg
Keyword(s):  
Bacillus Subtilis ◽  
Genetic Markers ◽  
Bacillus Licheniformis

scholarly journals CONGRESSION OF UNLINKED MARKERS AND GENETIC MAPPING IN THE TRANSFORMATION OF BACILLUS SUBTILIS 168

Genetics ◽  
1973 ◽  
Vol 73 (1) ◽  
pp. 13-21
Author(s):  
Robert J Erickson ◽  
James C Copeland
Keyword(s):  
Bacillus Subtilis ◽  
Genetic Mapping ◽  
Genetic Markers ◽  
Competent Cell ◽  
Dna Molecules ◽  
Bacillus Subtilis 168

ABSTRACT A thorough examination of cotransformation of two unlinked genetic markers in Bacillus subtilis 168 shows that the two recombinational events do not occur randomly. The cotransformation frequency is dependent on the distance between the two markers as well as on the order in which they replicate in the competent cell. These results indicate that uptake and/or integration of DNA molecules bearing these genetic markers is enhanced at the time these markers replicate in the competent cell.

scholarly journals Identification and Characterization of Novel Genetic Markers Associated with Biological Control Activities in Bacillus subtilis

2006 ◽  
Vol 96 (2) ◽  
pp. 145-154 ◽  
Author(s):  
Raghavendra Joshi ◽  
Brian B. McSpadden Gardener
Keyword(s):  
Biological Control ◽  
Bacillus Subtilis ◽  
Genetic Markers ◽  
Plant Pathogens ◽  
Plant Growth Promoting Rhizobacteria ◽  
Subtractive Hybridization ◽  
Pythium Ultimum ◽  
Suppressive Subtractive Hybridization ◽  
Antibiotic Biosynthesis ◽  
Wide Range

Suppressive subtractive hybridization (SSH) was used to identify genetic markers associated with biological control of plant pathogens by Bacillus subtilis. The genomes of two commercialized strains, GB03 and QST713, were compared with that of strain 168, which has no defined biocontrol capacities, to obtain a pool of DNA fragments unique to the two biocontrol strains. The sequences of 149 subtracted fragments were determined and compared with those present in GenBank, but only 80 were found to correspond to known Bacillus genes. Of these, 65 were similar to genes with a wide range of metabolic functions, including the biosynthesis of cell wall components, sporulation, and antibiotic biosynthesis. Sixteen subtracted fragments shared a high degree of similarity to sequences found in multiple B. subtilis strains with proven biocontrol capacities. Oligonucleotide primers specific to nine of these genes were developed. The targeted genes included five genes involved in antibiotic synthesis (bmyB, fenD, ituC,srfAA, and srfAB) and four additional genes (yndJ, yngG, bioA, and a hypothetical open reading frame) not previously associated with biological control. All nine markers were amplified from the commercialized B. subtilis strains GB03, QST713, and MBI600, with the exception of ituC, which was not detected in GB03. The markers also were amplified from four other B. subtilis isolates, but they were not amplified from other related Bacillus strains, including the plant growth-promoting rhizobacteria IN937a and IN937b. Sequencing of the amplified markers revealed that all seven of the isolates that scored positive for multiple markers were genotypically distinct strains. Interestingly, strains scored positive for the amplifiable markers generally were more effective at inhibiting the growth of Rhizoctonia solani and Pythium ultimum than other Bacillus isolates that lacked the markers. The potential utility of the defined genetic markers to further define the diversity, ecology, and biocontrol activities of B. subtilis are discussed.

Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

1980 ◽  
Vol 38 ◽  
pp. 540-541
Author(s):  
Dwight Anderson ◽  
Charlene Peterson ◽  
Gursaran Notani ◽  
Bernard Reilly
Keyword(s):  
Electron Microscopy ◽  
Bacillus Subtilis ◽  
Dna Synthesis ◽  
Restriction Endonuclease ◽  
Protein Complex ◽  
Protein Product ◽  
Viral Dna ◽  
Small Proteins ◽  
Antibody Labeling ◽  
Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

530: Termining Risk for Prostate Cancer at Prostate Biopsy using Genetic Markers

2005 ◽  
Vol 173 (4S) ◽  
pp. 144-145
Author(s):  
Robert K. Nam ◽  
William Zhang ◽  
John Trachtenberg ◽  
Michael A.S. Jewett ◽  
Steven Narod
Keyword(s):  
Prostate Cancer ◽  
Genetic Markers ◽  
Prostate Biopsy
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