Modification of l-phenylalanine ammonia-lyase in soybean cell suspension cultures by 2-aminooxyacetate and l-2-aminooxy-3-phenylpropionate

Planta ◽  
1981 ◽  
Vol 152 (2) ◽  
pp. 124-130 ◽  
Author(s):  
Evelyn A. Havir
Keyword(s):  
Cell Suspension ◽  
Phenylalanine Ammonia Lyase ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Soybean Cell

Metabolism of the Herbicide Isoproturon in Wheat and Soybean Cell Suspension Cultures

1999 ◽  
Vol 63 (2) ◽  
pp. 97-113 ◽  
Author(s):  
Werner Edgar Gläβgen ◽  
Dieter Komoβa ◽  
Olaf Bohnenkämper ◽  
Matthias Haas ◽  
Norbert Hertkorn ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Soybean Cell

Coordinated changes in enzyme activities of phenylpropanoid metabolism during the growth of soybean cell suspension cultures

1974 ◽  
Vol 362 (3) ◽  
pp. 417-424 ◽  
Author(s):  
Jürgen Ebel ◽  
Bärbel Schaller-Hekeler ◽  
Karl-Heinz Kobloch ◽  
Eckard Wellman ◽  
Hans Grisebach ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Enzyme Activities ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Phenylpropanoid Metabolism ◽  
Soybean Cell

Biosynthese von Cyanidin in Zellsuspensionskulturen von Haplopappus gracilis / Biosynthesis of Cyanidin in Cell Suspension Cultures of Haplopappus gracilis

1971 ◽  
Vol 26 (6) ◽  
pp. 581-585 ◽  
Author(s):  
Hansjörg Fritsch ◽  
Klaus Hahlbrock ◽  
Hans Grisebach
Keyword(s):  
Cell Suspension ◽  
Phenylalanine Ammonia Lyase ◽  
Cell Suspension Cultures ◽  
Maximal Activity ◽  
Suspension Cultures ◽  
Feeding Time ◽  
Haplopappus Gracilis ◽  
Time Required ◽  
Anthocyanin Production

Earlier work with buckwheat seedlings showed that dihydrokaempferol (3,5,7,4′-tetrahydroxyflavanone) (1) is a good precursor for cyanidin and quercetin. Since cell suspension cultures have several advantages for the quantitative comparison of the incorporation of a radioactive precursor into a product, the role of 1 in the biosynthesis of cyanidin was reinvestigated with cell cultures of Haplopappus gracilis. These cultures show good production of cyanidin-glycosides in blue light in a medium containing sucrose, 2,4-D and Fe-EDTA. Maximum anthocyanin production and maximal activity of phenylalanine ammonia-lyase is reached about 60 — 70 hrs. after the onset of illumination. The incorporation of 1 and of phenylalanine and 4,2′,4′,6′-tetrahydroxychalcone into cyanidin was compared under identical conditions after 1, 3 and 6 hrs. feeding time. 1 prooved to be by far the best precursor for cyanidin. The feeding time required was much shorter and the incorporation rates were much higher than in experiments with seedlings.

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