Light-induced flavone biosynthesis and activity of phenylalanine ammonia-lyase and UDP-apiose synthetase in cell suspension cultures of Petroselinum hortense

Planta ◽  
1970 ◽  
Vol 94 (3) ◽  
pp. 236-239 ◽  
Author(s):  
K. Hahlbrock ◽  
E. Wellmann
Keyword(s):  
Cell Suspension ◽  
Phenylalanine Ammonia Lyase ◽  
Cell Suspension Cultures ◽  
Suspension Cultures

Biosynthese von Cyanidin in Zellsuspensionskulturen von Haplopappus gracilis / Biosynthesis of Cyanidin in Cell Suspension Cultures of Haplopappus gracilis

1971 ◽  
Vol 26 (6) ◽  
pp. 581-585 ◽  
Author(s):  
Hansjörg Fritsch ◽  
Klaus Hahlbrock ◽  
Hans Grisebach
Keyword(s):  
Cell Suspension ◽  
Phenylalanine Ammonia Lyase ◽  
Cell Suspension Cultures ◽  
Maximal Activity ◽  
Suspension Cultures ◽  
Feeding Time ◽  
Haplopappus Gracilis ◽  
Time Required ◽  
Anthocyanin Production

Earlier work with buckwheat seedlings showed that dihydrokaempferol (3,5,7,4′-tetrahydroxyflavanone) (1) is a good precursor for cyanidin and quercetin. Since cell suspension cultures have several advantages for the quantitative comparison of the incorporation of a radioactive precursor into a product, the role of 1 in the biosynthesis of cyanidin was reinvestigated with cell cultures of Haplopappus gracilis. These cultures show good production of cyanidin-glycosides in blue light in a medium containing sucrose, 2,4-D and Fe-EDTA. Maximum anthocyanin production and maximal activity of phenylalanine ammonia-lyase is reached about 60 — 70 hrs. after the onset of illumination. The incorporation of 1 and of phenylalanine and 4,2′,4′,6′-tetrahydroxychalcone into cyanidin was compared under identical conditions after 1, 3 and 6 hrs. feeding time. 1 prooved to be by far the best precursor for cyanidin. The feeding time required was much shorter and the incorporation rates were much higher than in experiments with seedlings.

Elicitor-Induced Formation of Pterocarpan Phytoalexins in Chickpea (Cicer arietinum L.) Cell Suspension Cultures from Constitutive Isoflavone Conjugates upon Inhibition of Phenylalanine Ammonia Lyase

1991 ◽  
Vol 46 (1-2) ◽  
pp. 43-50 ◽  
Author(s):  
Ulrike Mackenbrock ◽  
Wolfgang Barz
Keyword(s):  
Cell Suspension ◽  
Cicer Arietinum ◽  
Phenylalanine Ammonia Lyase ◽  
De Novo ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Phenylpropionic Acid ◽  
Cicer Arietinum L ◽  
Physiological Conditions ◽  
L Cell

After inhibition of phenylalanine ammonia lyase by L- α-aminooxy-β-phenylpropionic acid, the constitutively formed formononetin 7-O-glucoside-6″-O-malonate is metabolized with the isoflavone aglycone being used as an intermediate in the elicitor-induced formation of pterocarpan phytoalexins in chickpea cell suspension cultures. In elicited cultures not treated with the inhibitor phytoalexins are synthesized de novo from phenylalanine. Therefore, in chickpea cells the constitutive isoflavone conjugate metabolism and the elicitor-induced pterocarpan formation show metabolic linkage under specific physiological conditions.

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