The initiation of legumin synthesis in immature embryos of Pisum sativum L. grown in vivo and in vitro

Planta ◽  
1980 ◽  
Vol 149 (5) ◽  
pp. 454-460 ◽  
Author(s):  
C. Domoney ◽  
D. R. Davies ◽  
R. Casey
Keyword(s):  
Pisum Sativum ◽  
Immature Embryos ◽  
Pisum Sativum L

In-vivo and in-vitro synthesis of photosynthetic fructose-1,6-bisphosphatase from pea (Pisum sativum L.)

Planta ◽  
1990 ◽  
Vol 182 (3) ◽  
pp. 319-324 ◽  
Author(s):  
M. Sahrawy ◽  
A. Chueca ◽  
R. Hermoso ◽  
J. J. Lázaro ◽  
J. López Gorgé
Keyword(s):  
Pisum Sativum ◽  
In Vitro Synthesis ◽  
Pisum Sativum L ◽  
Fructose 1,6 Bisphosphatase

In vitro and in vivo biological properties of pea pods (Pisum sativum L.)

2019 ◽  
Vol 32 ◽  
pp. 100482 ◽  
Author(s):  
Faiza Mejri ◽  
Haifa Ben Khoud ◽  
Leila Njim ◽  
Tarek Baati ◽  
Slimen Selmi ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Biological Properties ◽  
Pisum Sativum L

Nutritional assessment of raw and germinated pea (Pisum sativum L.) protein and carbohydrate by in vitro and in vivo techniques

Nutrition ◽  
2005 ◽  
Vol 21 (2) ◽  
pp. 230-239 ◽  
Author(s):  
Gloria Urbano ◽  
María López-Jurado ◽  
S.ławomir Frejnagel ◽  
Elena Gómez-Villalva ◽  
Jesús M. Porres ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Nutritional Assessment ◽  
L Protein ◽  
Pisum Sativum L

Intensification de la régénération du pois (Pisum sativum L.), par le thidiazuron, via la formation de structures caulinaires organogènes

1997 ◽  
Vol 75 (3) ◽  
pp. 492-500 ◽  
Author(s):  
Delphine Popiers ◽  
Frédéric Flandre ◽  
Brigitte S. Sangwan-Norreel
Keyword(s):  
Pisum Sativum ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Cotyledonary Nodes ◽  
Pisum Sativum L ◽  
Embryo Axes ◽  
Initial Medium ◽  
Second Wave ◽  
Mature Seeds

In vitro regeneration of pea (Pisum sativum L.), a regeneration recalcitrant legume, was optimised using thidiazuron. Buds were initiated from the meristems of the cotyledonary nodes of embryo axes, isolated from mature seeds, and subcultured on Murashige and Skoog medium supplemented with 13.3 μM 6-benzylaminopurine, 16.1 μM α-naphthaleneacetic acid, and 0.2 μM 2,3,5-triiodobenzoic acid. Proliferation of buds was preceded by the formation of white nodular-like protrusions. These structures were cut transversally in fine slices and subcultured on the same medium or in presence of thidiazuron that produces a second wave of secondary budding. The best results (90–110 buds per expiant) were obtained with 10 μM thidiazuron. The capacity of regeneration was genotype independent and reproducible. Buds elongated on the initial medium, then formed roots in presence of 5.37 μM α-naphthaleneacetic acid. and developed into viable plants. Key words: Pisum sativum L., regeneration, meristems, embryo axes, thidiazuron.

scholarly journals Protein synthesis in chloroplasts. Characteristics and products of protein synthesis in vitro in etioplasts and developing chloroplasts from pea leaves

1975 ◽  
Vol 146 (3) ◽  
pp. 675-685 ◽  
Author(s):  
S G Siddell ◽  
R J Ellis
Keyword(s):  
Energy Source ◽  
Protein Synthesis ◽  
Large Subunit ◽  
Sodium Dodecyl ◽  
Supernatant Fraction ◽  
Pisum Sativum L ◽  
Fraction I ◽  
Plastid Ribosomes

The function of plastid ribosomes in pea (Pisum sativum L.) was investigated by characterizing the products of protein synthesis in vitro in plastids isolated at different stages during the transition from etioplast to chloroplast. Etioplasts and plastids isolated after 24, 48 and 96h of greening in continuous white light, use added ATP to incorporate labelled amino acids into protein. Plastids isolated from greening leaves can also use light as the source of energy for protein synthesis. The labelled polypeptides synthesized in isolated plastids were analysed by electrophoresis in sodium dodecyl sulphate-ureapolyacrylamide gels. Six polypeptides are synthesized in etioplasts with ATP as energy source. Only one of these polypeptides is present in a 150 000g supernatant fraction. This polypeptide has been identified as the large subunit of Fraction I protein (3-phospho-D-glycerate carboxylyase EC 4.1.1.39) by comparing the tryptic ‘map’ of its L-(35S)methionine-labelled peptides with the tryptic ‘map’ of large subunit peptides from Fraction I labelled with L-(35S)methionine in vivo. The same gel pattern of six polypeptides is seen when plastids isolated from greening leaves are incubated with either added ATP or light as the energy source. However, the rates of synthesis of particular polypeptides are different in plastids isolated at different stages of the etioplast to chloroplast transition. The results support the idea that plastid ribosomes synthesize only a small number of proteins, and that the number and molecular weight of these proteins does not alter during the formation of chloroplasts from etioplasts.

scholarly journals Structural characterizations and in vitro digestibility of acid-treated wrinkled and smooth pea starch (Pisum sativum L.)

2016 ◽  
Vol 68 (7-8) ◽  
pp. 762-770 ◽  
Author(s):  
Miaomiao Shi ◽  
Kai Wang ◽  
Shujuan Yu ◽  
Robert G. Gilbert ◽  
Qunyu Gao
Keyword(s):  
Pisum Sativum ◽  
In Vitro Digestibility ◽  
Pisum Sativum L ◽  
Pea Starch ◽  
Structural Characterizations
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