HCO 3 ? -dependent ACh-activated Na+ influx in sheep parotid secretory endpieces

1995 ◽  
Vol 429 (6) ◽  
pp. 852-858 ◽  
Author(s):  
P. Poronnik ◽  
S. Y. Schumann ◽  
D. I. Cook
Keyword(s):  
Sheep Parotid

Sialography of sheep parotid and mandibular salivary glands

2000 ◽  
Vol 68 (1) ◽  
pp. 3-7 ◽  
Author(s):  
S.N. DEHGHANI ◽  
M. TADJALLI ◽  
M.H. MASOUMZADEH
Keyword(s):  
Salivary Glands ◽  
Sheep Parotid

scholarly journals Tissue and species distribution of the secreted carbonic anhydrase isoenzyme

1989 ◽  
Vol 259 (1) ◽  
pp. 91-96 ◽  
Author(s):  
R T Fernley ◽  
P Darling ◽  
P Aldred ◽  
R D Wright ◽  
J P Coghlan
Keyword(s):  
Acinar Cells ◽  
High Molecular Mass ◽  
Northern Analysis ◽  
Carbonic Anhydrases ◽  
Parotid Glands ◽  
Hybridization Histochemistry ◽  
Western Analysis ◽  
Mouse Sequence ◽  
Sheep Parotid ◽  
Aromatic Cluster

The secreted carbonic anhydrases, CA VI, are high molecular mass, oligomeric enzymes originally found in the sheep parotid gland and saliva. The enzymes have been purified from the saliva or parotid glands of several different species. All the CA VI enzymes studied have an apparent subunit Mr of about 45,000 as previously reported for the sheep enzyme. By Western analysis, CA VI from human, cow and dog cross-reacted with antibody raised against the purified sheep enzyme whereas that of the mouse did not. The N-terminal sequences of the sheep, human, cow and mouse enzymes are reported. The sheep, cow and human N-terminal sequences are similar to one another while the mouse sequence is substantially different. Nevertheless, the amino acids in the aromatic cluster I (Trp-5, Tyr-7, Trp-16 and Tyr/Phe-20) have all been conserved, as is the case with the cytoplasmic carbonic anhydrases. Eighteen tissues from the sheep have been examined for the presence of CA VI by Western analysis but it has been found only in the salivary glands. Northern analysis and hybridization histochemistry show that the mRNA for CA VI in sheep is expressed specifically in the acinar cells of the parotid and submandibular glands.

Molecular characterisation of the Na+/glucose co-transporter from the sheep parotid gland acinar cell

1994 ◽  
Vol 22 (3) ◽  
pp. 264S-264S ◽  
Author(s):  
PATRICK S. TARPEY ◽  
SORAYA P. SHIRAZI-BEECHEY ◽  
R. BRIAN BEECHEY
Keyword(s):  
Parotid Gland ◽  
Acinar Cell ◽  
Molecular Characterisation ◽  
Sheep Parotid

Preparation and characterization of a plasma-membrane fraction isolated from sheep parotid glands

1990 ◽  
Vol 18 (4) ◽  
pp. 646-646
Author(s):  
STEVEN VAYRO ◽  
SORAYA SHIRAZI-BEECHEY ◽  
RICHARD KEMP ◽  
R. BRIAN BEECHEY
Keyword(s):  
Plasma Membrane ◽  
Membrane Fraction ◽  
Parotid Glands ◽  
Plasma Membrane Fraction ◽  
Sheep Parotid

scholarly journals Preparation and characterization of basolateral plasma-membrane vesicles from sheep parotid glands. Mechanisms of phosphate and d-glucose transport

1991 ◽  
Vol 279 (3) ◽  
pp. 843-848 ◽  
Author(s):  
S Vayro ◽  
R Kemp ◽  
R B Beechey ◽  
S Shirazi-Beechey
Keyword(s):  
Basolateral Membrane ◽  
Membrane Vesicles ◽  
Acinar Cells ◽  
Tissue Homogenate ◽  
Basolateral Membrane Vesicles ◽  
Parotid Glands ◽  
Plasma Membrane Vesicles ◽  
Basolateral Plasma Membrane ◽  
Sheep Parotid

A procedure is described for the preparation of basolateral membrane vesicles from the acinar cells of the sheep parotid gland. The ouabain-sensitive K(+)-activated phosphatase activity was enriched 30-fold over the tissue homogenate; 45% of this activity was recovered in the final membrane fraction. The presence of membranes from other organelles was negligible. Evidence is presented for the location of Na(+)-dependent symporters for phosphate and D-glucose on the basolateral membrane.

The ultrastructure of the sheep parotid gland

1977 ◽  
Vol 179 (3) ◽  
pp. 377-392 ◽  
Author(s):  
Ernest W. van Lennep ◽  
Alan R. Kennerson ◽  
Jeffrey S. Compton
Keyword(s):  
Parotid Gland ◽  
Sheep Parotid

Na+ -H+ exchange in sheep parotid endpieces

FEBS Letters ◽  
1993 ◽  
Vol 315 (3) ◽  
pp. 307-312 ◽  
Author(s):  
P. Poronnik ◽  
J.A. Young ◽  
D.I. Cook
Keyword(s):  
Sheep Parotid
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