Molecular characterisation of the Na+/glucose co-transporter from the sheep parotid gland acinar cell

1994 ◽  
Vol 22 (3) ◽  
pp. 264S-264S ◽  
Author(s):  
PATRICK S. TARPEY ◽  
SORAYA P. SHIRAZI-BEECHEY ◽  
R. BRIAN BEECHEY
Keyword(s):  
Parotid Gland ◽  
Acinar Cell ◽  
Molecular Characterisation ◽  
Sheep Parotid

Cell-Surface Galactosyltransferase Acts as a Modulator of Rat and Human Acinar Cell Proliferation

1990 ◽  
Vol 4 (1) ◽  
pp. 45-60 ◽  
Author(s):  
M.G. Humphreys-Beher ◽  
T. Zelles ◽  
N. Maeda ◽  
K.R. Purushotham ◽  
N. Cassisi ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Cell Surface ◽  
Parotid Gland ◽  
Adenoid Cystic Carcinoma ◽  
Acinar Cell ◽  
Egf Receptor ◽  
Neoplastic Tissue ◽  
Direct Role ◽  
Adenoid Cystic

Several physiological parameters were examined for inducing acinar cell proliferation and corresponding increased expression of β1-4 galactosyltransferase. In this study, dietary changes causing acinar cell proliferation included the following: the introduction of animals to a liquid diet (causing gland atrophy) followed by re-introduction of solid chow, gustatory stimulation provided by the introduction of 0.5% citric acid to animal drinking water, and removal of the submandibular gland with subsequent reliance on the parotid gland for saliva protein and fluid. Alterations in growth factor levels were produced by injecting animals with a chronic (three-day) regimen of either nerve growth factor (NGF) or epidermal growth factor (EGF). In all cases of acinar cell proliferation in vivo, generated by the above treatments, cell-surface galactosyltransferase was detected along with the unique expression of a 4.5-kb proliferation-associated mRNA. Parotid gland proliferation could be blocked in all cases by the injection of the galactosyltransferase specific modifier protein, a-lactalbumin. Propranolol, a β-adrenergic receptor antagonist, blocked proliferation in all cases except EGF treatment. EGFinduced proliferation could, however, be prevented if the animals were treated with monoclonal antibody to EGF receptor or with the galactosyltransferase modifier a-lactalbumin. As a comparison, human parotid tissue samples obtained from neoplastic pleomorphic adenomas, mucoepidermoid carcinoma, adenoid cystic carcinoma, and a bulimia patient were analyzed for galactosyltransferase expression by Northern blot of mRNA and plasma membrane isolation. Elevated levels of galactosyltransferase were found in all neoplastic tissue preparations as well as in the bulimia sample. Amylase synthesis was reduced in samples compared with surrounding normal tissue from the same patient. In vitro cell culturing of pleomorphic adenoma cells in the presence of galactosyltransferase modifier a-lactalbumin and substrate UDP-galactose inhibited proliferation in a dose-dependent fashion. Southern blot analysis of DNA from neoplastic parotid cells showed an alteration in chromosomal gene structure for the galactosyltransferase activator cDNA from the adenoid cystic carcinoma. These results for induced acinar cell proliferation as well as human neoplastic pathologies suggest a direct role for cell surface β1-4 galactosyltransferase in signaling growth. Furthermore, the proliferation-associated activity of galactosyltransferase suggests that it may be considered as a new type of cell growth regulator.

The ultrastructure of the sheep parotid gland

1977 ◽  
Vol 179 (3) ◽  
pp. 377-392 ◽  
Author(s):  
Ernest W. van Lennep ◽  
Alan R. Kennerson ◽  
Jeffrey S. Compton
Keyword(s):  
Parotid Gland ◽  
Sheep Parotid

Significance of Nodal Metastasis in Parotid Gland Acinar Cell Carcinoma

2020 ◽  
Author(s):  
Peter Moon ◽  
Mahbuba Tusty ◽  
Vasu Divi ◽  
Uchechukwu C. Megwalu
Keyword(s):  
Parotid Gland ◽  
Cell Carcinoma ◽  
Acinar Cell ◽  
Acinar Cell Carcinoma ◽  
Nodal Metastasis

scholarly journals Purification and characterization of a high-Mr carbonic anhydrase from sheep parotid gland

1988 ◽  
Vol 249 (1) ◽  
pp. 201-207 ◽  
Author(s):  
R T Fernley ◽  
J P Coghlan ◽  
R D Wright
Keyword(s):  
Carbonic Anhydrase ◽  
Parotid Gland ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Carbonic Anhydrase Ii ◽  
Carbonic Anhydrases ◽  
Protein Subunit ◽  
N Terminus ◽  
Sheep Parotid

Approximately half the carbonic anhydrase activity of sheep parotid-gland homogenate is derived from a high-Mr protein [Fernley, Wright & Coghlan (1979) FEBS Lett. 105, 299-302]. This enzyme has now been purified to homogeneity, and its properties were compared with those of the well-characterized sheep carbonic anhydrase II. The protein has an apparent Mr of 540,000 as measured by gel filtration under non-denaturing conditions and an apparent subunit Mr of 45,000 as measured by SDS/polyacrylamide-gel electrophoresis. After deglycosylation with the enzyme N-glycanase the protein migrates with an apparent Mr of 36,000 on SDS/polyacrylamide-gel electrophoresis. The CO2-hydrating activity was 340 units/mg compared with 488 units/mg for sheep carbonic anhydrase II measured under identical conditions. This enzyme does not, however, hydrolyse p-nitrophenyl acetate. The enzyme contains 0.8 g-atom of zinc/mol of protein subunit. The peptide maps of the two carbonic anhydrases differ significantly from one another, indicating they are not related closely structurally. Unlike the carbonic anhydrase II isoenzyme, which has a blocked N-terminus, the high-Mr enzyme has a free glycine residue at its N-terminus.

ELIMINATION OF RADIOIODINE BY THE SHEEP PAROTID GLAND AFTER [131I]l-TRI-IODOTHYRONINE ADMINISTRATION

1972 ◽  
Vol 52 (3) ◽  
pp. 427-434
Author(s):  
A. ŚLEBODZIŃSKI
Keyword(s):  
Parotid Gland ◽  
Salivary Glands ◽  
Gel Filtration ◽  
Parotid Glands ◽  
Sheep Parotid

SUMMARY A study was made of the metabolism of [131I]l-tri-iodothyronine ([131I]T3), in sheep with a unilateral fistula of the parotid gland. After [131I]T3 injection a constant rise in radioactivity in the saliva and a decline in the radioactivity of the serum were found; detectable amounts of radioiodine reentered the circulation from the metabolized hormone after 1 h. Gel filtration showed that the radioactivity in the collected saliva was due to inorganic [131I]iodide. Such an effect might be caused by deiodination [131I]T3 which occurs in sheep parotid glands in vitro. The presence of tracer amounts of hormonal radioiodine in individual sheep could not be excluded. Non-specific deiodinating enzymes may exist in sheep parotid salivary glands.

Sign in / Sign up

Export Citation Format

Share Document