The ultrastructure of the sheep parotid gland

1977 ◽  
Vol 179 (3) ◽  
pp. 377-392 ◽  
Author(s):  
Ernest W. van Lennep ◽  
Alan R. Kennerson ◽  
Jeffrey S. Compton
Keyword(s):  
Parotid Gland ◽  
Sheep Parotid

Molecular characterisation of the Na+/glucose co-transporter from the sheep parotid gland acinar cell

1994 ◽  
Vol 22 (3) ◽  
pp. 264S-264S ◽  
Author(s):  
PATRICK S. TARPEY ◽  
SORAYA P. SHIRAZI-BEECHEY ◽  
R. BRIAN BEECHEY
Keyword(s):  
Parotid Gland ◽  
Acinar Cell ◽  
Molecular Characterisation ◽  
Sheep Parotid

scholarly journals Purification and characterization of a high-Mr carbonic anhydrase from sheep parotid gland

1988 ◽  
Vol 249 (1) ◽  
pp. 201-207 ◽  
Author(s):  
R T Fernley ◽  
J P Coghlan ◽  
R D Wright
Keyword(s):  
Carbonic Anhydrase ◽  
Parotid Gland ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Carbonic Anhydrase Ii ◽  
Carbonic Anhydrases ◽  
Protein Subunit ◽  
N Terminus ◽  
Sheep Parotid

Approximately half the carbonic anhydrase activity of sheep parotid-gland homogenate is derived from a high-Mr protein [Fernley, Wright & Coghlan (1979) FEBS Lett. 105, 299-302]. This enzyme has now been purified to homogeneity, and its properties were compared with those of the well-characterized sheep carbonic anhydrase II. The protein has an apparent Mr of 540,000 as measured by gel filtration under non-denaturing conditions and an apparent subunit Mr of 45,000 as measured by SDS/polyacrylamide-gel electrophoresis. After deglycosylation with the enzyme N-glycanase the protein migrates with an apparent Mr of 36,000 on SDS/polyacrylamide-gel electrophoresis. The CO2-hydrating activity was 340 units/mg compared with 488 units/mg for sheep carbonic anhydrase II measured under identical conditions. This enzyme does not, however, hydrolyse p-nitrophenyl acetate. The enzyme contains 0.8 g-atom of zinc/mol of protein subunit. The peptide maps of the two carbonic anhydrases differ significantly from one another, indicating they are not related closely structurally. Unlike the carbonic anhydrase II isoenzyme, which has a blocked N-terminus, the high-Mr enzyme has a free glycine residue at its N-terminus.

ELIMINATION OF RADIOIODINE BY THE SHEEP PAROTID GLAND AFTER [131I]l-TRI-IODOTHYRONINE ADMINISTRATION

1972 ◽  
Vol 52 (3) ◽  
pp. 427-434
Author(s):  
A. ŚLEBODZIŃSKI
Keyword(s):  
Parotid Gland ◽  
Salivary Glands ◽  
Gel Filtration ◽  
Parotid Glands ◽  
Sheep Parotid

SUMMARY A study was made of the metabolism of [131I]l-tri-iodothyronine ([131I]T3), in sheep with a unilateral fistula of the parotid gland. After [131I]T3 injection a constant rise in radioactivity in the saliva and a decline in the radioactivity of the serum were found; detectable amounts of radioiodine reentered the circulation from the metabolized hormone after 1 h. Gel filtration showed that the radioactivity in the collected saliva was due to inorganic [131I]iodide. Such an effect might be caused by deiodination [131I]T3 which occurs in sheep parotid glands in vitro. The presence of tracer amounts of hormonal radioiodine in individual sheep could not be excluded. Non-specific deiodinating enzymes may exist in sheep parotid salivary glands.

Tetraethylammonium blocks muscarinically evoked secretion in the sheep parotid gland by a mechanism additional to its blockade of BK channels

1992 ◽  
Vol 420 (2) ◽  
pp. 167-171 ◽  
Author(s):  
D. I. Cook ◽  
E. A. Wegman ◽  
T. Ishikawa ◽  
P. Poronnik ◽  
D. G. Allen ◽  
...  
Keyword(s):  
Parotid Gland ◽  
Bk Channels ◽  
Sheep Parotid

Ultrastructure of the Parotid Gland of the Nine-Banded Armadillo

1977 ◽  
Vol 35 ◽  
pp. 640-641
Author(s):  
J. R. Ruby
Keyword(s):  
Endoplasmic Reticulum ◽  
Parotid Gland ◽  
Periodic Acid ◽  
Acinar Cells ◽  
Duct System ◽  
Parotid Glands ◽  
Dasypus Novemcinctus ◽  
Anterior Portion ◽  
Periodic Acid Schiff ◽  
Thin Sections

Parotid glands were obtained from five adult (four male and one female) armadillos (Dasypus novemcinctus) which were perfusion-fixed. The glands were located in a position similar to that of most mammals. They extended interiorly to the anterior portion of the submandibular gland.In the light microscope, it was noted that the acini were relatively small and stained strongly positive with the periodic acid-Schiff (PAS) and alcian blue techniques, confirming the earlier results of Shackleford (1). Based on these qualities and other structural criteria, these cells have been classified as seromucous (2). The duct system was well developed. There were numerous intercalated ducts and intralobular striated ducts. The striated duct cells contained large amounts of PAS-positive substance.Thin sections revealed that the acinar cells were pyramidal in shape and contained a basally placed, slightly flattened nucleus (Fig. 1). The rough endoplasmic reticulum was also at the base of the cell.

Ultrastructure of myoepithelial cell in parotid gland

1988 ◽  
Vol 46 ◽  
pp. 342-343
Author(s):  
C. N. Sun
Keyword(s):  
Parotid Gland ◽  
Osmium Tetroxide ◽  
Individual Cell ◽  
Branching Processes ◽  
Muscle Cells ◽  
Myoepithelial Cells ◽  
Uranyl Acetate ◽  
Thin Sections ◽  
Gland Carcinoma ◽  
Parotid Gland Carcinoma

Myoepithelial cells have been observed in the prostate, harderian, apocrine, exocrine sweat and mammary glands. Such cells and their numerous branching processes form basket-like structures around the glandular acini. Their shapes are quite different from structures seen either in spindleshaped smooth muscle cells or skeletal muscle cells. These myoepithelial cells lie on the epithelial side of the basement membrane in the glands. This presentation describes the ultrastructure of such myoepithelial cells which have been found also in the parotid gland carcinoma from a 45-year old patient.Specimens were cut into small pieces about 1 mm3 and immediately fixed in 4 percent glutaraldehyde in phosphate buffer for two hours, then post-fixed in 1 percent buffered osmium tetroxide for 1 hour. After dehydration, tissues were embedded in Epon 812. Thin sections were stained with uranyl acetate and lead citrate. Ultrastructurally, the pattern of each individual cell showed wide variations.

MR-Elastography of the Parotid Gland: Feasibility and First Experiences

2006 ◽  
Vol 177 (01) ◽  
Author(s):  
CR Habermann ◽  
R Sinkus ◽  
A Albrecht ◽  
MC Cramer ◽  
F Weiss ◽  
...  
Keyword(s):  
Parotid Gland ◽  
Mr Elastography

A Case of Sclerosing Polycystic Adenosis of Parotid Gland

2014 ◽  
Vol 57 (8) ◽  
pp. 559
Author(s):  
Young-Jun Kim ◽  
Jang-Won Choi ◽  
Young-Joong Kim ◽  
Soo-Kweon Koo
Keyword(s):  
Parotid Gland
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