Cytological localization of the genes for the four classes of ribosomal RNA (25S, 18S, 5.8S and 5S) in polytene chromosomes of Phaseolus coccineus

Chromosoma ◽  
1977 ◽  
Vol 60 (3) ◽  
pp. 269-282 ◽  
Author(s):  
M. Durante ◽  
P. G. Cionini ◽  
S. Avanzi ◽  
R. Cremonini ◽  
F. D'Amato
Keyword(s):  
Ribosomal Rna ◽  
Polytene Chromosomes ◽  
Phaseolus Coccineus ◽  
Cytological Localization

Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus coccineus by fluorescence in situ hybridization after pepsin pretreatment

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 1018-1021 ◽  
Author(s):  
M. Nenno ◽  
K. Schumann ◽  
W. Nagl
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Polytene Chromosomes ◽  
Seed Storage ◽  
Phaseolus Coccineus ◽  
Rna Genes

This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The structural preservation of the chromosomes and the distinctness of the FISH signals were comparatively analysed with a probe for the ribosomal RNA genes after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a general loosening of chromatin with good conservation of chromosome morphology and an increased number and density of signal points. The six nucleolus organizers exhibited significant differences in condensation. The pretreatment with pepsin enabled the detection of the low-copy genes encoding the seed storage protein phaseolin.Key words: plant, Leguminosae, ribosomal RNA genes, seed storage protein genes, protease.

Cytological localization of ribosomal cistrons in polytene chromosomes of Phaseolus coccineus

Chromosoma ◽  
1972 ◽  
Vol 39 (2) ◽  
pp. 191-203 ◽  
Author(s):  
S. Avanzi ◽  
M. Durante ◽  
P. G. Cionini ◽  
F. D'Amato
Keyword(s):  
Polytene Chromosomes ◽  
Phaseolus Coccineus ◽  
Ribosomal Cistrons ◽  
Cytological Localization

Cytological localization of DNA complementary to ribosomal RNA in polytene chromosomes of Diptera

Chromosoma ◽  
1970 ◽  
Vol 29 (3) ◽  
pp. 268-290 ◽  
Author(s):  
Mary Lou Pardue ◽  
Susan A. Gerbi ◽  
Ronald A. Eckhardt ◽  
Joseph G. Gall
Keyword(s):  
Ribosomal Rna ◽  
Polytene Chromosomes ◽  
Cytological Localization

Quantitative in situ hybridization of ribosomal RNA species to polytene chromosomes of Drosophila melanogaster

1977 ◽  
Vol 115 (3) ◽  
pp. 539-563 ◽  
Author(s):  
Paul Szabo ◽  
Robert Elder ◽  
Dale M. Steffensen ◽  
Olke C. Uhlenbeck
Keyword(s):  
Drosophila Melanogaster ◽  
In Situ Hybridization ◽  
Ribosomal Rna ◽  
Polytene Chromosomes

Ribosomal RNA genes of Phaseolus coccineus. I

1992 ◽  
Vol 18 (6) ◽  
pp. 1073-1082 ◽  
Author(s):  
Fabio Maggini ◽  
Gianfranco Tucci ◽  
Anna Demartis ◽  
Maria Teresa Gelati ◽  
Silvana Avanzi
Keyword(s):  
Ribosomal Rna ◽  
Phaseolus Coccineus ◽  
Ribosomal Rna Genes ◽  
Rna Genes
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