Cytological localization of DNA complementary to ribosomal RNA in polytene chromosomes of Diptera

Chromosoma ◽  
1970 ◽  
Vol 29 (3) ◽  
pp. 268-290 ◽  
Author(s):  
Mary Lou Pardue ◽  
Susan A. Gerbi ◽  
Ronald A. Eckhardt ◽  
Joseph G. Gall
Keyword(s):  
Ribosomal Rna ◽  
Polytene Chromosomes ◽  
Cytological Localization

Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus coccineus by fluorescence in situ hybridization after pepsin pretreatment

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 1018-1021 ◽  
Author(s):  
M. Nenno ◽  
K. Schumann ◽  
W. Nagl
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Polytene Chromosomes ◽  
Seed Storage ◽  
Phaseolus Coccineus ◽  
Rna Genes

This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The structural preservation of the chromosomes and the distinctness of the FISH signals were comparatively analysed with a probe for the ribosomal RNA genes after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a general loosening of chromatin with good conservation of chromosome morphology and an increased number and density of signal points. The six nucleolus organizers exhibited significant differences in condensation. The pretreatment with pepsin enabled the detection of the low-copy genes encoding the seed storage protein phaseolin.Key words: plant, Leguminosae, ribosomal RNA genes, seed storage protein genes, protease.

Cytological localization of ribosomal cistrons in polytene chromosomes of Phaseolus coccineus

Chromosoma ◽  
1972 ◽  
Vol 39 (2) ◽  
pp. 191-203 ◽  
Author(s):  
S. Avanzi ◽  
M. Durante ◽  
P. G. Cionini ◽  
F. D'Amato
Keyword(s):  
Polytene Chromosomes ◽  
Phaseolus Coccineus ◽  
Ribosomal Cistrons ◽  
Cytological Localization

Quantitative in situ hybridization of ribosomal RNA species to polytene chromosomes of Drosophila melanogaster

1977 ◽  
Vol 115 (3) ◽  
pp. 539-563 ◽  
Author(s):  
Paul Szabo ◽  
Robert Elder ◽  
Dale M. Steffensen ◽  
Olke C. Uhlenbeck
Keyword(s):  
Drosophila Melanogaster ◽  
In Situ Hybridization ◽  
Ribosomal Rna ◽  
Polytene Chromosomes

Localization of the 5S RNA genes to arm 2R in polytene chromosomes of Lucilia cuprina (Diptera: Calliphoridae)

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 941-943 ◽  
Author(s):  
D. G. Bedo ◽  
G. C. Webb
Keyword(s):  
In Situ Hybridization ◽  
Ribosomal Rna ◽  
Polytene Chromosomes ◽  
Lucilia Cuprina ◽  
Chromosome 2 ◽  
5S Rna ◽  
Rna In Situ Hybridization ◽  
5S Rna Genes ◽  
Rna Genes

The 5S RNA genes of Lucilia cuprina were mapped to section 15A in the short arm of chromosome 2 by in situ hybridization to pupal trichogen polytene cells. As in most eukaryotes the 5S genes are located separately from the remaining ribosomal RNA genes.Key words: Lucilia cuprina, 5S RNA, in situ hybridization.

Visualisation of E. coli ribosomal RNA in situ by electron spectroscopic imaging and image averaging

1992 ◽  
Vol 50 (1) ◽  
pp. 466-467
Author(s):  
Daniel Beniac ◽  
George Harauz
Keyword(s):  
Ribosomal Rna ◽  
Three Dimensional ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
E Coli ◽  
Microscopical Technique ◽  
Quantitative Image ◽  
Dimensional Reconstruction ◽  
Image Averaging ◽  
Protein Components

The structures of E. coli ribosomes have been extensively probed by electron microscopy of negatively stained and frozen hydrated preparations. Coupled with quantitative image analysis and three dimensional reconstruction, such approaches are worthwhile in defining size, shape, and quaternary organisation. The important question of how the nucleic acid and protein components are arranged with respect to each other remains difficult to answer, however. A microscopical technique that has been proposed to answer this query is electron spectroscopic imaging (ESI), in which scattered electrons with energy losses characteristic of inner shell ionisations are used to form specific elemental maps. Here, we report the use of image sorting and averaging techniques to determine the extent to which a phosphorus map of isolated ribosomal subunits can define the ribosomal RNA (rRNA) distribution within them.

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