The detection of activated cerebrospinal fluid B lymphocytes by peroxidase conjugated antibodies

1980 ◽  
Vol 224 (2) ◽  
pp. 77-87 ◽  
Author(s):  
H. -J. Schädlich ◽  
M. Nekić ◽  
K. Felgenhauer
Keyword(s):  
Cerebrospinal Fluid ◽  
B Lymphocytes

scholarly journals Differential Diagnosis of Autoimmune Encephalitis from Infectious Lymphocytic Encephalitis by Analysing the Lymphocyte Subsets of Cerebrospinal Fluid

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Qiao-quan Zhang ◽  
Yan-fang Zhang ◽  
Nian Yu ◽  
Xing-jian Lin ◽  
Qing Di
Keyword(s):  
Cerebrospinal Fluid ◽  
B Lymphocytes ◽  
Lymphocyte Subsets ◽  
Developmental Stages ◽  
Autoimmune Encephalitis ◽  
Herpes Simplex Virus Encephalitis ◽  
Pathophysiological Mechanism ◽  
Diagnostic Values ◽  
Simplex Virus ◽  
Cd Markers

This study is aimed at investigating the lymphocyte subsets of cerebrospinal fluid (CSF) to provide possible differential diagnostic values and better understand the pathophysiological mechanism underlying autoimmune encephalitis (AE) and infectious lymphocytic encephalitis. A series of CD markers, including CD3/4/8/20 representing different types and developmental stages of lymphocytes, were used to count the corresponding subpopulations of CSF from clinical and laboratory confirmed cases of anti-N-methyl-D-aspartate receptor AE (NMDAR-AE), herpes simplex virus encephalitis (HSVE), and tuberculous meningitis (TBM). The percentages of lymphocytes observed and the CD4 : CD8 ratios were compared between the three groups. There were no significant differences of the percentage of total lymphocytes, CD3 cells, and CD4 cells of CSF among each group. However, there were strongly statistical differences of the CD4 : CD8 ratio in CSF of each group with 0.6 : 1 in NMDAR-AE, 0.9 : 1 in HSVE, and 3.2 : 1 in TBM. The percentage of CD20 B lymphocytes in NMDAR-AE was statistically higher than that of other groups. The distinct percentages of lymphocyte subpopulations of CSF appeared to be characteristic and could potentially serve as diagnostic indicators. Further verification and research will be necessary to clarify the significance and nature of CD4 : CD8 ratios and B lymphocytes in CSF between AE and the infectious lymphocytic encephalitis.

scholarly journals Accumulation of Clonally Related B Lymphocytes in the Cerebrospinal Fluid of Multiple Sclerosis Patients

2000 ◽  
Vol 164 (5) ◽  
pp. 2782-2789 ◽  
Author(s):  
Monica Colombo ◽  
Mariella Dono ◽  
Paola Gazzola ◽  
Silvio Roncella ◽  
Angelo Valetto ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
B Lymphocytes ◽  
Multiple Sclerosis Patients

Specific antigen binding by activated cerebrospinal fluid B lymphocytes in acute neuroborreliosis

1992 ◽  
Vol 239 (6) ◽  
pp. 322-326 ◽  
Author(s):  
Wolfgang Beuche ◽  
Arno Siever ◽  
Klaus Felgenhauer
Keyword(s):  
Cerebrospinal Fluid ◽  
B Lymphocytes ◽  
Specific Antigen ◽  
Antigen Binding

scholarly journals Immunopathogenetic features of bacterial purulent meningitides

2014 ◽  
Vol 19 (5) ◽  
pp. 4-9
Author(s):  
I. P Balmasova ◽  
Yu. Ya Vengerov ◽  
S. E Razdobarina ◽  
M. V Nagibina
Keyword(s):  
Immune Response ◽  
Cerebrospinal Fluid ◽  
B Lymphocytes ◽  
Pneumococcal Infection ◽  
Absolute Number ◽  
Comparative Assessment ◽  
Cns Infection ◽  
Purulent Meningitis ◽  
The Absolute ◽  
The Impact

The aim of the study. Comparative assessment of the state of cells of the immune system in patients with purulent bacterial meningitides caused by N.meningitidis and S.pneumoniae . Materials and methods. By the method offlow cytofluorometry there were tested blood and cerebrospinal fluid of 65 patients with bacterial purulent meningitis, with the meningococcal (38 people) and pneumococcal (27 people) nature of the disease. Results. There were revealed general consistencies of the immune response to CNS infection with meningococcus andpneumococcus: the fall of the number of CD16+ CD56+(NK) and the gain in the number of CD19+ cells in the blood, an increase in the absolute number of T lymphocytes, especially, CD3+ CD8+ and NK. In pneumococcal meningitis there was noted the more pronounced upsurge in the absolute number of the NKT (CD3+CD56+) and B-lymphocytes in the blood whereas in cerebrospinal fluid - the more pronounced upturn in the absolute number of NKT that testified to the severe course of the disease. Conclusion. As a result of performed studies there was identified a series of consistencies for the development of the immune response in bacterial purulent meningitides of meningococcal and pneumococcal etiology, which show the high informativeness of the study of immunograms of cerebrospinal fluid in these diseases as compared with blood. In the latter case, immunological studies may contribute not only to the identification of general consistencies of the development of the immune response in bacterial meningitis, but also serve as a basis for the differentiation of the impact of meningococcal and pneumococcal infection on immunocompetent cells, as well as to reflect the severity of the course of the disease, affecting the nature of the therapy.

The proliferation rate of T and B lymphocytes in cerebrospinal fluid

1991 ◽  
Vol 238 (1) ◽  
pp. 27-30 ◽  
Author(s):  
R. S. Thomas ◽  
W. Beuche ◽  
K. Felgenhauer
Keyword(s):  
Cerebrospinal Fluid ◽  
B Lymphocytes ◽  
Proliferation Rate ◽  
T And B Lymphocytes
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