Detection of malignant B lymphocytes by PCR clonality assay using direct lysis of cerebrospinal fluid and low volume specimens

2014 ◽  
Vol 37 (2) ◽  
pp. 165-173 ◽  
Author(s):  
L. Liu ◽  
F. Cao ◽  
S. Wang ◽  
J. Zhou ◽  
G. Yang ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
B Lymphocytes ◽  
Low Volume ◽  
Pcr Clonality

scholarly journals Differential Diagnosis of Autoimmune Encephalitis from Infectious Lymphocytic Encephalitis by Analysing the Lymphocyte Subsets of Cerebrospinal Fluid

2019 ◽  
Vol 2019 ◽  
pp. 1-6
Author(s):  
Qiao-quan Zhang ◽  
Yan-fang Zhang ◽  
Nian Yu ◽  
Xing-jian Lin ◽  
Qing Di
Keyword(s):  
Cerebrospinal Fluid ◽  
B Lymphocytes ◽  
Lymphocyte Subsets ◽  
Developmental Stages ◽  
Autoimmune Encephalitis ◽  
Herpes Simplex Virus Encephalitis ◽  
Pathophysiological Mechanism ◽  
Diagnostic Values ◽  
Simplex Virus ◽  
Cd Markers

This study is aimed at investigating the lymphocyte subsets of cerebrospinal fluid (CSF) to provide possible differential diagnostic values and better understand the pathophysiological mechanism underlying autoimmune encephalitis (AE) and infectious lymphocytic encephalitis. A series of CD markers, including CD3/4/8/20 representing different types and developmental stages of lymphocytes, were used to count the corresponding subpopulations of CSF from clinical and laboratory confirmed cases of anti-N-methyl-D-aspartate receptor AE (NMDAR-AE), herpes simplex virus encephalitis (HSVE), and tuberculous meningitis (TBM). The percentages of lymphocytes observed and the CD4 : CD8 ratios were compared between the three groups. There were no significant differences of the percentage of total lymphocytes, CD3 cells, and CD4 cells of CSF among each group. However, there were strongly statistical differences of the CD4 : CD8 ratio in CSF of each group with 0.6 : 1 in NMDAR-AE, 0.9 : 1 in HSVE, and 3.2 : 1 in TBM. The percentage of CD20 B lymphocytes in NMDAR-AE was statistically higher than that of other groups. The distinct percentages of lymphocyte subpopulations of CSF appeared to be characteristic and could potentially serve as diagnostic indicators. Further verification and research will be necessary to clarify the significance and nature of CD4 : CD8 ratios and B lymphocytes in CSF between AE and the infectious lymphocytic encephalitis.

scholarly journals Accumulation of Clonally Related B Lymphocytes in the Cerebrospinal Fluid of Multiple Sclerosis Patients

2000 ◽  
Vol 164 (5) ◽  
pp. 2782-2789 ◽  
Author(s):  
Monica Colombo ◽  
Mariella Dono ◽  
Paola Gazzola ◽  
Silvio Roncella ◽  
Angelo Valetto ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Cerebrospinal Fluid ◽  
B Lymphocytes ◽  
Multiple Sclerosis Patients

Specific antigen binding by activated cerebrospinal fluid B lymphocytes in acute neuroborreliosis

1992 ◽  
Vol 239 (6) ◽  
pp. 322-326 ◽  
Author(s):  
Wolfgang Beuche ◽  
Arno Siever ◽  
Klaus Felgenhauer
Keyword(s):  
Cerebrospinal Fluid ◽  
B Lymphocytes ◽  
Specific Antigen ◽  
Antigen Binding

scholarly journals What is the current clinical practise in pituitary adenoma surgery in Europe? European Pituitary Adenoma Surgery Survey (EU-PASS) results - technical part

2021 ◽  
Author(s):  
Martin Májovský ◽  
Andre Grotenhuis ◽  
Nicolas Foroglou ◽  
Francesco Zenga ◽  
Sebastien Froehlich ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Pituitary Adenoma ◽  
Cerebrospinal Fluid Leak ◽  
Personal Communication ◽  
High Volume ◽  
Future Research ◽  
Postoperative Mri ◽  
Geographical Regions ◽  
Low Volume ◽  
Fluid Leak

Abstract Pituitary adenoma surgery has evolved rapidly in recent decades, changing clinical practice markedly and raising new challenges. There is no current consensus or guidelines for perioperative care that includes possible complication management. This study aims to determine current practice across European neurosurgical centers. We created a list of eligible departments performing pituitary adenoma surgery based on cooperation with EANS, Νational neurosurgical societies, and personal communication with local neurosurgeons. We contacted the chairpersons from each department and asked them (or another responsible neurosurgeon) to fill out the survey. The survey consisted of 58 questions. For further analysis, departments were divided into subgroups: “academic”/”non-academic center”, “high-volume”/”low-volume”, “mainly endoscopic”/“mainly microscopic”/“mixed practise” and by geographical regions. Data from 254 departments from 34 countries were obtained. The average time to complete the survey was 18 min. Notably, the endoscopic approach is the predominant surgical approach in Europe, used in 56.8% of the centers. In routine cases without intraoperative cerebrospinal fluid leak, high-volume centres are less pedantic with sellar closure when compared with low-volume centres. On the other hand, when a postoperative cerebrospinal fluid leak occurs, high-volume centres are more active and indicate early reoperation. Less than 15% of the participating centres perform early postoperative MRI scans. Marked variation was noted among different groups of respondents and some contentious issues are discussed. Such information can encourage useful debate about the reasons for the variations seen and perhaps help develop standardised protocols to improve patient outcomes. A future research focus is to compare European results with other regions.

scholarly journals Testing for Herpes Simplex Virus in Low-Volume Cerebrospinal Fluid Samples: Comparison of Three Protocols To Optimize Detection

2015 ◽  
Vol 53 (12) ◽  
pp. 3897-3899 ◽  
Author(s):  
Mark J. Espy ◽  
Cole L. Irish ◽  
Matthew J. Binnicker
Keyword(s):  
Cerebrospinal Fluid ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Medical Emergency ◽  
Herpes Simplex Virus 1 ◽  
Pcr Method ◽  
Low Volume ◽  
Simplex Virus ◽  
Testing Protocols ◽  
Hsv 1

Detection of herpes simplex virus 1 and 2 (HSV-1 and HSV-2) in cerebrospinal fluid (CSF) is a medical emergency and requires rapid, sensitive testing. However, the volume of CSF received for microbiological studies may be limited, especially from young children. In this study, we compared three testing protocols to our routine real-time PCR method to determine the most sensitive approach for detecting HSV-1 and HSV-2 in low-volume (≤100 μl) CSF.

The detection of activated cerebrospinal fluid B lymphocytes by peroxidase conjugated antibodies

1980 ◽  
Vol 224 (2) ◽  
pp. 77-87 ◽  
Author(s):  
H. -J. Schädlich ◽  
M. Nekić ◽  
K. Felgenhauer
Keyword(s):  
Cerebrospinal Fluid ◽  
B Lymphocytes
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