Chromosomal localization of ribosomal 5 S RNA genes in Chironomus thummi by in situ hybridization of iodinated 5S RNA

Chromosoma ◽  
1976 ◽  
Vol 57 (2) ◽  
pp. 199-204 ◽  
Author(s):  
Helmut B�umlein ◽  
Ulrich Wobus
Keyword(s):  
In Situ Hybridization ◽  
Chromosomal Localization ◽  
5S Rna ◽  
Rna Genes ◽  
Chironomus Thummi

Localization of the 5S RNA genes to arm 2R in polytene chromosomes of Lucilia cuprina (Diptera: Calliphoridae)

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 941-943 ◽  
Author(s):  
D. G. Bedo ◽  
G. C. Webb
Keyword(s):  
In Situ Hybridization ◽  
Ribosomal Rna ◽  
Polytene Chromosomes ◽  
Lucilia Cuprina ◽  
Chromosome 2 ◽  
5S Rna ◽  
Rna In Situ Hybridization ◽  
5S Rna Genes ◽  
Rna Genes

The 5S RNA genes of Lucilia cuprina were mapped to section 15A in the short arm of chromosome 2 by in situ hybridization to pupal trichogen polytene cells. As in most eukaryotes the 5S genes are located separately from the remaining ribosomal RNA genes.Key words: Lucilia cuprina, 5S RNA, in situ hybridization.

scholarly journals Chromosomal localization of human genes for arylamine N-acetyltransferase

1994 ◽  
Vol 297 (3) ◽  
pp. 441-445 ◽  
Author(s):  
D Hickman ◽  
A Risch ◽  
V Buckle ◽  
N K Spurr ◽  
S J Jeremiah ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
In Situ Hybridization ◽  
Yeast Artificial Chromosome ◽  
Chromosomal Localization ◽  
Artificial Chromosome ◽  
Acetylator Phenotype ◽  
Chromosome 8 ◽  
Slow Acetylator ◽  
Human Genes

Arylamine N-acetyltransferase is encoded at two loci, AAC-1 and AAC-2, on human chromosome 8. The products of the two loci are able to catalyse N-acetylation of arylamine carcinogens, such as benzidine and other xenobiotics. AAC-2 is polymorphic and individuals carrying the slow-acetylator phenotype are more susceptible to benzidine-induced bladder cancer. We have identified yeast artificial chromosome clones encoding AAC-1 and AAC-2 and have used the cloned DNAs as fluorescent probes for in situ hybridization. The hybridization patterns allow assignment of AAC-1 and AAC-2 to chromosome 8p21.3-23.1, a region in which deletions have been associated with bladder cancer [Knowles, Shaw and Proctor (1993) Oncogene 8, 1357-1364].

Chromosomal localization of leukocyte interferon gene in the pig (Sus scrofa domestica L.) by in situ hybridization

1986 ◽  
Vol 42 (3) ◽  
pp. 129-132 ◽  
Author(s):  
M. Yerle ◽  
J. Gellin ◽  
G. Echard ◽  
F. Lefevre ◽  
M. Gillois
Keyword(s):  
In Situ Hybridization ◽  
Sus Scrofa ◽  
Chromosomal Localization ◽  
Interferon Gene

Detection of rRNA and phaseolin genes on polytene chromosomes of Phaseolus coccineus by fluorescence in situ hybridization after pepsin pretreatment

Genome ◽  
1994 ◽  
Vol 37 (6) ◽  
pp. 1018-1021 ◽  
Author(s):  
M. Nenno ◽  
K. Schumann ◽  
W. Nagl
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Ribosomal Rna ◽  
Storage Protein ◽  
Seed Storage Protein ◽  
Polytene Chromosomes ◽  
Seed Storage ◽  
Phaseolus Coccineus ◽  
Rna Genes

This is the first report of fluorescence in situ hybridization (FISH) on plant polytene chromosomes. Different protease pretreatments have been tested to improve fluorescence in situ hybridization FISH on polytene chromosomes of a plant, Phaseolus coccineus, with the aim to enable the detection of low-copy genes. The structural preservation of the chromosomes and the distinctness of the FISH signals were comparatively analysed with a probe for the ribosomal RNA genes after digestion with pepsin and trypsin. The pepsin pretreatment resulted in a general loosening of chromatin with good conservation of chromosome morphology and an increased number and density of signal points. The six nucleolus organizers exhibited significant differences in condensation. The pretreatment with pepsin enabled the detection of the low-copy genes encoding the seed storage protein phaseolin.Key words: plant, Leguminosae, ribosomal RNA genes, seed storage protein genes, protease.

Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization

Nature ◽  
1985 ◽  
Vol 317 (6033) ◽  
pp. 175-177 ◽  
Author(s):  
J. E. Landegent ◽  
N. Jansen in de Wal ◽  
G.-J. B. van Omment ◽  
F. Baas ◽  
J. J. M. de Vijlderi ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Chromosomal Localization ◽  
Unique Gene

Chromosomal localization of human lactotransferrin gene (LTF) by in situ hybridization

1988 ◽  
Vol 47 (1-2) ◽  
pp. 16-17 ◽  
Author(s):  
J.L. McCombs ◽  
C.T. Teng ◽  
B.T. Pentecost ◽  
V.L. Magnuson ◽  
C.M. Moore ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Chromosomal Localization
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