Effect of cycloheximide on RNA synthesis in Chironomus polytene chromosomes

Chromosoma ◽  
1977 ◽  
Vol 61 (4) ◽  
pp. 369-379 ◽  
Author(s):  
J. L. D�ez ◽  
M. C. Santa-Cruz ◽  
P. Aller
Keyword(s):  
Rna Synthesis ◽  
Polytene Chromosomes

Comparison of in vivo and in vitro RNA synthesis on polytene chromosomes of Drosophila

Chromosoma ◽  
1976 ◽  
Vol 54 (4) ◽  
pp. 349-362 ◽  
Author(s):  
B. A. Leibovitch ◽  
E. S. Belyaeva ◽  
I. F. Zhimulev ◽  
R. B. Khesin
Keyword(s):  
Rna Synthesis ◽  
Polytene Chromosomes

scholarly journals RNA SYNTHESIS BY EXOGENOUS RNA POLYMERASE ON CYTOLOGICAL PREPARATIONS OF CHROMOSOMES

1973 ◽  
Vol 57 (2) ◽  
pp. 538-550 ◽  
Author(s):  
R. Sederoff ◽  
R. Clynes ◽  
M. Poncz ◽  
S. Hachtel
Keyword(s):  
Rna Polymerase ◽  
Dna Sequences ◽  
Rna Synthesis ◽  
Actinomycin D ◽  
Polytene Chromosomes ◽  
Polymerase Activity ◽  
Exogenous Rna ◽  
Rna Polymerase Activity ◽  
Chromosome Regions

Cytological preparations of Drosophila polytene chromosomes serve as templates for RNA synthesis carried out by exogenous RNA polymerase (Escherichia coli). Incorporation of labeled ribonucleoside triphosphates into RNA may be observed directly by autoradiography. Because of the effects of rifampicin, actinomycin D, ribonuclease, high salt, and the requirement for all four nucleoside triphosphates, we conclude that the labeling observed over chromosomes is due to DNA-dependent RNA polymerase activity. Using this method, one can observe RNA synthesis in vitro on specific chromosome regions due to the activity of exogenous RNA polymerase. We find that much of the RNA synthesis in this system occurs on DNA sequences which appear to be in a nondenatured state.

Chromosomal RNA synthesis in polytene chromosomes of Chironomus tentans

Chromosoma ◽  
1969 ◽  
Vol 28 (4) ◽  
Author(s):  
B. Daneholt ◽  
J.-E. Edstr�m ◽  
E. Egyh�zi ◽  
B. Lambert ◽  
U. Ringborg
Keyword(s):  
Rna Synthesis ◽  
Polytene Chromosomes ◽  
Chironomus Tentans
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