Induction of heat-shock Balbiani Rings after RNA synthesis inhibition in polytene chromosomes of Chironomus thummi

Chromosoma ◽  
1982 ◽  
Vol 87 (5) ◽  
pp. 507-517 ◽  
Author(s):  
Domingo Barettino ◽  
Gloria Morcillo ◽  
Jos�-Luis Di�z
Keyword(s):  
Heat Shock ◽  
Rna Synthesis ◽  
Polytene Chromosomes ◽  
Synthesis Inhibition ◽  
Balbiani Rings ◽  
Chironomus Thummi

Selective stimulation of puffing activity in vitro with a juvenile hormone analogue by polytene chromosomes in the salivary glands of Chironomus thummi (Diptera)

1989 ◽  
Vol 67 (10) ◽  
pp. 2528-2532
Author(s):  
X. Vafopoulou ◽  
C. G. H. Steel ◽  
H. Laufer
Keyword(s):  
Juvenile Hormone ◽  
Salivary Glands ◽  
Polytene Chromosomes ◽  
Hormone Analogue ◽  
Juvenile Hormone Analogue ◽  
Balbiani Rings ◽  
Chironomus Thummi ◽  
Stimulation Of

Salivary glands of Chironomus thummi prepupae were treated in vitro with various concentrations of the juvenile hormone analogue (JHA) methoprene and the puffing activities of Balbiani rings (BR) b and c were scored in cytological preparations of polytene chromosomes. In control cultures, both BRb and BRc regress rapidly. JHA treatment in vitro prevented regression at both these sites. In addition, BRb was found to expand within 60 min to a size two to three times larger than in control contralateral glands. The most effective concentration for stimulation of BRb and BRc in vitro was 10−7 M. In vivo treatment of prepupae with 10−7 M JHA also induced puffing activity in BRb and BRc to a degree similar to that observed following in vitro treatment. It is concluded that BRb, and perhaps also BRc, are juvenile hormone (JH) inducible chromosomal sites in Chironomus thummi salivary glands. This is the first report of chromosomal sites that are stimulated by JH in vitro in the absence of exogenous ecdysteroids. These findings support the view that JH may act at the gene level at separate loci from ecdysone.

The heat shock protein hsp70 binds in vivo to subregions 2-48BC and 3-58D of the polytene chromosomes ofDrosophila hydei

Chromosoma ◽  
1990 ◽  
Vol 99 (5) ◽  
pp. 315-320 ◽  
Author(s):  
Elizabeth Laran ◽  
José Maria Requena ◽  
Antonio Jimenez-Ruiz ◽  
Manuel Carlos Lopez ◽  
Carlos Alonso
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Polytene Chromosomes ◽  
Heat Shock Protein Hsp70

scholarly journals Association of a protease with polytene chromosomes of Drosophila melanogaster.

1980 ◽  
Vol 87 (2) ◽  
pp. 415-419 ◽  
Author(s):  
J Cavagnaro ◽  
D A Pierce ◽  
J C Lucchesi ◽  
C B Chae
Keyword(s):  
Acetic Acid ◽  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Protease Inhibitors ◽  
Polytene Chromosomes ◽  
Fixation Procedure ◽  
Apparent Effect ◽  
Chloromethyl Ketone ◽  
Diisopropyl Fluorophosphate ◽  
Uniform Labeling

Incubation of Drosophila salivary glands with radioactive diisopropyl fluorophosphate results in the uniform labeling of polytene chromosomes. Extensive labeling is seen only when chromosome squashes are prepared by a formaldehyde fixation procedure and not by standard acetic acid techniques. The labeling is inhibited in the presence of tosylphenylalanine chloromethyl ketone and phenylmethane sulfonylfluoride but not by tosyllysine chloromethyl ketone, suggesting that a chymotrypsin-like serine protease is associated with the chromosomes. Protease inhibitors show no apparent effect on heat-shock specific puffing.

scholarly journals Superhelical DNA of polytene chromosomes Chironomus thummi

1988 ◽  
Vol 4 (3) ◽  
pp. 129-133
Author(s):  
M. A. Shurdov ◽  
A. D. Gruzdev
Keyword(s):  
Polytene Chromosomes ◽  
Chironomus Thummi

DNA and RNA content of Chironomus thummi polytene chromosomes determined by micro column gel filtration

1975 ◽  
Vol 3 (6) ◽  
pp. 361-370 ◽  
Author(s):  
Edgard Serfling ◽  
Vladimir I. Majorov ◽  
Nikolai I. Mikichur ◽  
Taissya G. Popova ◽  
Lev S. Sandakchiev
Keyword(s):  
Polytene Chromosomes ◽  
Gel Filtration ◽  
Dna And Rna ◽  
Rna Content ◽  
Chironomus Thummi

Heat-shock induced puffing changes in Balbiani rings

Chromosoma ◽  
1971 ◽  
Vol 32 (2) ◽  
Author(s):  
Hiroshi Yamamoto
Keyword(s):  
Heat Shock ◽  
Balbiani Rings

HSP90 associates with specific heat shock puffs (hsr?) in polytene chromosomes of Drosophila and Chironomus

Chromosoma ◽  
1993 ◽  
Vol 102 (9) ◽  
pp. 648-659 ◽  
Author(s):  
Gloria Morcillo ◽  
Jos� Luis Diez ◽  
M. Eloisa Carbajal ◽  
Robert M. Tanguay
Keyword(s):  
Heat Shock ◽  
Specific Heat ◽  
Polytene Chromosomes
Sign in / Sign up

Export Citation Format

Share Document