Chromosomal RNA synthesis in polytene chromosomes of Chironomus tentans

Chromosoma ◽  
1969 ◽  
Vol 28 (4) ◽  
Author(s):  
B. Daneholt ◽  
J.-E. Edstr�m ◽  
E. Egyh�zi ◽  
B. Lambert ◽  
U. Ringborg
Keyword(s):  
Rna Synthesis ◽  
Polytene Chromosomes ◽  
Chironomus Tentans

RNA Synthesis in a Balbiani Ring in Chironomus tentans

1970 ◽  
Vol 35 (0) ◽  
pp. 513-519 ◽  
Author(s):  
B. Daneholt ◽  
J.-E. Edstrom ◽  
E. Egyhazi ◽  
B. Lambert ◽  
U. Ringborg
Keyword(s):  
Rna Synthesis ◽  
Chironomus Tentans ◽  
Balbiani Ring

scholarly journals Mobility restriction in vivo of the heavy ribosomal subunit in a secretory cell.

1976 ◽  
Vol 70 (3) ◽  
pp. 573-580 ◽  
Author(s):  
U Lönn ◽  
J E Edström
Keyword(s):  
Endoplasmic Reticulum ◽  
Ribosomal Rna ◽  
Rna Synthesis ◽  
Ribosomal Subunit ◽  
Chironomus Tentans ◽  
Salivary Gland Cells ◽  
Unique Parameter ◽  
Labeled Rna

Analysis in insect (Chironomus tentans) salivary gland cells of labeled RNA as a function of time after precursor injection and its distance to the nuclear membrane, cytoplasmic zone analysis, could previously be used to demonstrate the presence of short-lasting gradients in newly labeled ribosomal RNA. Since the gradients were sensitive to puromycin, they are likely to be a result of diffusion restriction due to an engagement of the subunits into polysomes. In the present paper the possibility was explored of recording gradients that were caused by labeled subunits in puromycin-resistant associations, which, in all probability, involve the endoplasmic reticulum. It was found that labeled 28 S and 5 S RNA but not 18 S RNA were present in radioactivity gradients lasting for at least 2 days but less than 6 days. The gradients also remained during inhibition of RNA synthesis by actinomycin, and they were completely resistant to puromycin whether given in vivo or in vitro. The semipermanent gradients formed fhere offer a unique parameter for the in vivo study of conditions for formation and maintenance of heavy subunits in puromycin-resistant bonds. An explanation for these and previous results is that the light subunit, although restricted in movement by engagement to polysomes, is nevertheless free to exchange and spread between rounds of translation, whereas at least part of the heavy subunit population is bound to the endoplasmic reticulum and less free to spread. These results offer a good in vivo correlate to previous results on in vitro exchangeability of subunits.

Inactive chromatin in polytene chromosomes of Chironomus tentans partially shows a non-nucleosomal organization

Chromosoma ◽  
1986 ◽  
Vol 94 (1) ◽  
pp. 19-30 ◽  
Author(s):  
Endre Egyh�zi ◽  
Johan Thyberg ◽  
Andrew Pigon ◽  
Olle Johansson
Keyword(s):  
Polytene Chromosomes ◽  
Chironomus Tentans ◽  
Inactive Chromatin

Effect of cycloheximide on RNA synthesis in Chironomus polytene chromosomes

Chromosoma ◽  
1977 ◽  
Vol 61 (4) ◽  
pp. 369-379 ◽  
Author(s):  
J. L. D�ez ◽  
M. C. Santa-Cruz ◽  
P. Aller
Keyword(s):  
Rna Synthesis ◽  
Polytene Chromosomes
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