Anther culture and Hordeum bulbosum-derived barley doubled haploids mutations and methylation

1993 ◽  
Vol 241-241 (5-6) ◽  
pp. 674-679 ◽  
Author(s):  
Pierre Devaux ◽  
Andrzej Kilian ◽  
Andris Kleinhofs
Keyword(s):  
Anther Culture ◽  
Doubled Haploids ◽  
Hordeum Bulbosum

Genotype and Environment Strongly Influence Barley Anther Culture Response Using Australian Genotypes

1993 ◽  
Vol 41 (2) ◽  
pp. 227 ◽  
Author(s):  
SJ Logue ◽  
LC Giles ◽  
DHB Sparrow
Keyword(s):  
Anther Culture ◽  
Doubled Haploids ◽  
Growth Conditions ◽  
Green Plant ◽  
Optimal Conditions ◽  
Donor Plant ◽  
Breeding Programs ◽  
Barley Cultivars ◽  
Large Numbers ◽  
Anther Culture Response

A screening of several Australian barley cultivars of commercial interest has identified a number of genotypes that respond well to anther culture, with average levels of green plant regeneration between 23 and 134 plants/100 anthers cultured. Donor plant growth conditions have a large impact on anther culture response and, although optimal conditions for specific genotypes could possibly be identified, it is likely to be more effective for the production of large numbers of doubled haploids to settle for a broadly acceptable environment. Recent advances in methodology and the identification of responsive genotypes makes anther culture a feasible procedure for Australian barley breeding programs.

Improved production of doubled haploids by colchicine application to wheat ( Triticum aestivum L.) anther culture

1998 ◽  
Vol 17 (12) ◽  
pp. 974-979 ◽  
Author(s):  
A. Redha ◽  
T. Attia ◽  
B. Büter ◽  
S. Saisingtong ◽  
P. Stamp ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Doubled Haploids ◽  
Triticum Aestivum L ◽  
Colchicine Application

Diallel analysis of barley anther culture response

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 152-157 ◽  
Author(s):  
W. Powell
Keyword(s):  
Anther Culture ◽  
Doubled Haploids ◽  
Diallel Analysis ◽  
Green Plant ◽  
Plant Production ◽  
Microspore Development ◽  
Culture Techniques ◽  
Plantlet Formation ◽  
Breeding Programmes ◽  
Anther Culture Response

The genetics of barley microspore development in culture was examined by means of diallel analysis. The frequency of microspore derived green and albino plant production was shown to be under genetic control. This genotypic limitation to microspore development will limit the application of anther culture techniques to barley breeding programmes. However, significant additive genetic effects were detected for the characters measured and indicate that the frequency of green plant regeneration may be improved by the hybridization of suitable parents. Significant reciprocal differences were also detected and indicate that the direction of the cross is important in determining microspore development. An embryogenic route to green plantlet formation was observed in a number of genotypes in the diallel experiment. The implications of these findings for barley improvement and genetics are discussed.Key words: doubled haploids, barley, anther culture, microspore, embryoid.

scholarly journals Doubled Haploids generated through anther culture from an elite long duration rice hybrid, CRHR32: Method optimization and molecular characterization

2016 ◽  
Vol 33 (3) ◽  
pp. 177-186 ◽  
Author(s):  
Prachitara Rout ◽  
Nupur Naik ◽  
Umakanta Ngangkham ◽  
Ram Lakhan Verma ◽  
Jawahar Lal Katara ◽  
...  
Keyword(s):  
Anther Culture ◽  
Molecular Characterization ◽  
Doubled Haploids ◽  
Long Duration ◽  
Rice Hybrid ◽  
Method Optimization

scholarly journals In vitro androgenesis in triticale

2017 ◽  
Vol 1 (1) ◽  
pp. 99-100
Author(s):  
Oksana Ivanovna Zaitseva
Keyword(s):  
Genetic Variation ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Doubled Haploids ◽  
External Factors ◽  
Hexaploid Triticale ◽  
Doubled Haploid Lines ◽  
In Vitro Androgenesis

Abstract The technology for in vitro anther culturing was optimized in hexaploid triticale using combination of external factors that allowed to obtain more than 100 doubled haploid lines. Investigation of genetic variation among anther culture derived doubled haploids of triticale showed the occurrence of heterozygous plants.

scholarly journals Pembentukan Genotipe Padi Berumur Sangat Genjah melalui Kultur Antera

2016 ◽  
Vol 18 (2) ◽  
pp. 54
Author(s):  
Iswari S. Dewi ◽  
A. Dinar Ambarwati ◽  
Aniversari Apriana ◽  
Atmitri Sisharmini ◽  
Ida H. Somantri ◽  
...  
Keyword(s):  
Anther Culture ◽  
Callus Induction ◽  
Culture Media ◽  
Oryza Sativa L ◽  
Doubled Haploids ◽  
Heading Date ◽  
Population Increase ◽  
Early Maturity ◽  
Early Maturing ◽  
Pure Lines

<p>Development of Very Early Maturing Rice Genotypes through Anther Culture. Iswari S. Dewi, A. Dinar Ambarwati, Aniversari Apriana, Atmitri Sisharmini, Ida H. Somantri, Bambang Suprihatno, and Iman Ridwan. Rice is the most important food crop in Indonesia. Increase in production is needed due to population increase. Rice production in rainfed area is contributed the second after irrigated area. Rainfed condition requiring very early maturity (90-104 days) varieties. Rice anther culture can be applied to accelerate obtainment of doubled haploids (DHs) or pure lines needed in rice breeding. The experiment was aimed to obtain pure lines for developing very early maturing and high yielding rice varieties. Materials used for anther culture were F1s of Fatmawati/Kinamase, Inpari 1/Kinamase, Fatmawati/ Waseaikoku, Inpari 1/Waseaikoku, Fatmawati/IR71146, Inpari 1/IR71146, OM4495/Silugonggo, IR7146/Dodokan, and IR71730/OM1490. Anther culture media were N6 + NAA 2,0 mg/l + kinetin 0,5 mg/l for callus induction, MS+ NAA 0,5 mg/l + kinetin 2,0 mg/l for plantlet regeneration, and MS + 0,5 mg/l IBA for rooting. Putrescine 10-3 M was added to callus induction and regeneration media. The results shown that calli forming green plantlet (CFGP) were ranged from 0.25 to 83.33%. Fatmawati/Kinamase gave the highest CFGP (245 calli), followed by Inpari 1/Kinamase (78 calli) and Fatmawati/ Waseaikoku (68 calli). Total green plantlets obtained were 2.038 plantlets. After plantlet acclimatization and greenhouse grow-out, we obtained 507 DHs. The evaluation of 100 DHs at farmer field (Ciranjang District in Cianjur), based on their 50% heading date of 65 days, resulted in 33 lines cathegorized as very early maturing lines (+100 days). They were 18 lines from Fatmawati/Kinamase, 5 lines from Inpari 1/Kinamase, 8 lines from Fatmawati/Waseaikoku, and 2 lines from Inpari 1/ Waseaikoku.</p><p> </p><p><strong>Abstrak</strong></p><p>Padi (Oryza sativa L.) merupakan komoditi pangan terpenting di Indonesia. Peningkatan produksi diperlukan seiring dengan peningkatan jumlah penduduk. Lahan sawah tadah hujan merupakan lumbung padi kedua setelah sawah irigasi. Kondisi lahan sawah tadah hujan memerlukan varietas-varietas padi berumur sangat genjah (90-104 hari). Teknik kultur antera dapat digunakan untuk mempercepat perolehan tanaman dihaploid (DH) atau galur murni dalam pemuliaan padi. Penelitian ini bertujuan untuk mendapatkan galur-galur murni yang akan digunakan dalam perakitan padi berdaya hasil tinggi dan berumur sangat genjah. Bahan tanaman yang digunakan untuk kultur antera adalah malai dari tanaman F1 hasil persilangan Fatmawati/Kinamase, Inpari 1/Kinamase, Fatmawati/Waseaikoku, Inpari 1/Waseaikoku, Fatmawati/IR71146, Inpari 1/ IR71146, OM4495/Silugonggo, IR7146/Dodokan, dan IR71730/OM1490. Media kultur antera adalah N6 + NAA 2,0 mg/l + kinetin 0,5 mg/l untuk media induksi kalus, MS+ NAA 0,5 mg/l + kinetin 2,0 mg/l untuk media regenerasi, dan MS + 0,5 mg/l IBA untuk media perakaran. Putresine 10-3 M ditambahkan pada media induksi kalus dan regenerasi. Hasil penelitian menunjukkan bahwa kalus yang menghasilkan tanaman hijau (KMTH) berkisar antara 0,25-83,33%. Persilangan Fatmawati/ Kinamase memberikan KMTH tertinggi (245 kalus), diikuti oleh Inpari 1/Kinamase (78 kalus) dan Fatmawati/ Waseaikoku (68 kalus). Total tanaman hijau yang diperoleh adalah 2.038 planlet dihaploid, namun diperoleh 507 tanaman setelah planlet diaklimatisasi dan tanaman ditumbuhkan di rumah kaca. Evaluasi terhadap 100 DH dilakukan di lahan petani Ciranjang, Cianjur. Berdasarkan hari berbunga 50% (65 hari setelah semai), diperoleh 33 galur yang termasuk kategori sangat genjah (dipanen +100 hari). Galur-galur tersebut adalah 18 galur dari persilangan Fatmawati/Kinamase, 5 galur dari persilangan Inpari 1/Kinamase, 8 galur dari persilangan Fatmawati/ Waseaikoku, dan 2 galur dari persilangan Inpari 1/ Waseaikoku.</p>

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