A dominant selectable marker that is meiotically stable in Neurospora crassa: the amdS gene of Aspergillus nidulans

1992 ◽  
Vol 236 (1) ◽  
pp. 121-124 ◽  
Author(s):  
Carl T. Yamashiro ◽  
Oded Yarden ◽  
Charles Yanofsky
Keyword(s):  
Neurospora Crassa ◽  
Aspergillus Nidulans ◽  
Selectable Marker ◽  
Amds Gene ◽  
Dominant Selectable Marker

scholarly journals Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker.

1986 ◽  
Vol 6 (7) ◽  
pp. 2452-2461 ◽  
Author(s):  
M J Orbach ◽  
E B Porro ◽  
C Yanofsky
Keyword(s):  
Neurospora Crassa ◽  
Selectable Marker ◽  
Resistant Mutant ◽  
Intron Position ◽  
Coding Region ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Benomyl Resistance ◽  
Dominant Selectable Marker ◽  
Beta Tubulin Gene

We cloned the beta-tubulin gene of Neurospora crassa from a benomyl-resistant strain and determined its nucleotide sequence. The gene encodes a 447-residue protein which shows strong homology to other beta-tubulins. The coding region is interrupted by six introns, five of which are within the region coding for the first 54 amino acids of the protein. Intron position comparisons between the N. crassa gene and other fungal beta-tubulin genes reveal considerable positional conservation. The mutation responsible for benomyl resistance was determined; it caused a phenylalanine-to-tyrosine change at position 167. Codon usage in the beta-tubulin gene is biased, as has been observed for other abundantly expressed N. crassa genes such as am and the H3 and H4 histone genes. This bias results in pyrimidines in the third positions of 96% of the codons in codon families in which there is a choice between purines and pyrimidines in this position. Bias is also evident by the absence of 19 of the 61 sense codons. We demonstrated that benomyl resistance is due to the cloned beta-tubulin gene of strain Bml511(r)a and that this gene can be used as a dominant selectable marker in N. crassa transformation.

Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker

1986 ◽  
Vol 6 (7) ◽  
pp. 2452-2461
Author(s):  
M J Orbach ◽  
E B Porro ◽  
C Yanofsky
Keyword(s):  
Neurospora Crassa ◽  
Selectable Marker ◽  
Resistant Mutant ◽  
Intron Position ◽  
Coding Region ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Benomyl Resistance ◽  
Dominant Selectable Marker ◽  
Beta Tubulin Gene

We cloned the beta-tubulin gene of Neurospora crassa from a benomyl-resistant strain and determined its nucleotide sequence. The gene encodes a 447-residue protein which shows strong homology to other beta-tubulins. The coding region is interrupted by six introns, five of which are within the region coding for the first 54 amino acids of the protein. Intron position comparisons between the N. crassa gene and other fungal beta-tubulin genes reveal considerable positional conservation. The mutation responsible for benomyl resistance was determined; it caused a phenylalanine-to-tyrosine change at position 167. Codon usage in the beta-tubulin gene is biased, as has been observed for other abundantly expressed N. crassa genes such as am and the H3 and H4 histone genes. This bias results in pyrimidines in the third positions of 96% of the codons in codon families in which there is a choice between purines and pyrimidines in this position. Bias is also evident by the absence of 19 of the 61 sense codons. We demonstrated that benomyl resistance is due to the cloned beta-tubulin gene of strain Bml511(r)a and that this gene can be used as a dominant selectable marker in N. crassa transformation.

Bialaphos resistance as a dominant selectable marker in Neurospora crassa

1989 ◽  
Vol 16 (5-6) ◽  
pp. 369-372 ◽  
Author(s):  
Javier Avalos ◽  
Robert F. Geever ◽  
Mary E. Case
Keyword(s):  
Neurospora Crassa ◽  
Selectable Marker ◽  
Dominant Selectable Marker

scholarly journals Utilization of the Aspergillus nidulans pyrG gene as a selectable marker for transformation and electroporation of Neurospora crassa

1997 ◽  
Vol 44 (1) ◽  
pp. 57-59 ◽  
Author(s):  
G. E. Turner ◽  
T. J. Jimenez ◽  
S. K. Chae ◽  
R. A. Baasiri ◽  
K. A. Borkovich
Keyword(s):  
Neurospora Crassa ◽  
Aspergillus Nidulans ◽  
Selectable Marker

Hygromycin B resistance as dominant selectable marker in yeast

1984 ◽  
Vol 8 (5) ◽  
pp. 353-358 ◽  
Author(s):  
Kevin R. Kaster ◽  
Stanley G. Burgett ◽  
Thomas D. Ingolia
Keyword(s):  
Selectable Marker ◽  
Hygromycin B ◽  
Hygromycin B Resistance ◽  
Dominant Selectable Marker

Insertional mutagenesis in Coprinus cinereus : use of a dominant selectable marker to generate tagged, sporulation-defective mutants

1999 ◽  
Vol 36 (6) ◽  
pp. 371-382 ◽  
Author(s):  
W. Jason Cummings ◽  
Martina Celerin ◽  
Jennifer Crodian ◽  
Linda K. Brunick ◽  
Miriam E. Zolan
Keyword(s):  
Insertional Mutagenesis ◽  
Selectable Marker ◽  
Coprinus Cinereus ◽  
Dominant Selectable Marker

The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation

1994 ◽  
Vol 14 (6) ◽  
pp. 4011-4019
Author(s):  
J A Nelson ◽  
P B Savereide ◽  
P A Lefebvre
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Selectable Marker ◽  
Marker Gene ◽  
Small Subunit ◽  
Direct Selection ◽  
Nuclear Transformation ◽  
Ribulose Bisphosphate Carboxylase ◽  
Bisphosphate Carboxylase ◽  
Dominant Selectable Marker ◽  
Cry1 Gene

We have cloned and sequenced the CRY1 gene, encoding ribosomal protein S14 in Chlamydomonas reinhardtii, and found that it is highly similar to S14/rp59 proteins from other organisms, including mammals, Drosophila melanogaster, and Saccharomyces cerevisiae. We isolated a mutant strain resistant to the eukaryotic translational inhibitors cryptopleurine and emetine in which the resistance was due to a missense mutation (CRY1-1) in the CRY1 gene; resistance was dominant in heterozygous stable diploids. Cotransformation experiments using the CRY1-1 gene and the gene for nitrate reductase (NIT1) produced a low level of resistance to cryptopleurine and emetine. Resistance levels were increased when the CRY1-1 gene was placed under the control of a constitutive promoter from the ribulose bisphosphate carboxylase/oxygenase small subunit 2 (RBCS2) gene. We also found that the 5' untranslated region of the CRY1 gene was required for expression of the CRY1-1 transgene. Direct selection of emetine-resistant transformants was possible when transformed cells were first induced to differentiate into gametes by nitrogen starvation and then allowed to dedifferentiate back to vegetative cells before emetine selection was applied. With this transformation protocol, the RBCS2/CRY1-1 dominant selectable marker gene is a powerful tool for many molecular genetic applications in C. reinhardtii.

Sign in / Sign up

Export Citation Format

Share Document