scholarly journals Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker.

1986 ◽  
Vol 6 (7) ◽  
pp. 2452-2461 ◽  
Author(s):  
M J Orbach ◽  
E B Porro ◽  
C Yanofsky
Keyword(s):  
Neurospora Crassa ◽  
Selectable Marker ◽  
Resistant Mutant ◽  
Intron Position ◽  
Coding Region ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Benomyl Resistance ◽  
Dominant Selectable Marker ◽  
Beta Tubulin Gene

We cloned the beta-tubulin gene of Neurospora crassa from a benomyl-resistant strain and determined its nucleotide sequence. The gene encodes a 447-residue protein which shows strong homology to other beta-tubulins. The coding region is interrupted by six introns, five of which are within the region coding for the first 54 amino acids of the protein. Intron position comparisons between the N. crassa gene and other fungal beta-tubulin genes reveal considerable positional conservation. The mutation responsible for benomyl resistance was determined; it caused a phenylalanine-to-tyrosine change at position 167. Codon usage in the beta-tubulin gene is biased, as has been observed for other abundantly expressed N. crassa genes such as am and the H3 and H4 histone genes. This bias results in pyrimidines in the third positions of 96% of the codons in codon families in which there is a choice between purines and pyrimidines in this position. Bias is also evident by the absence of 19 of the 61 sense codons. We demonstrated that benomyl resistance is due to the cloned beta-tubulin gene of strain Bml511(r)a and that this gene can be used as a dominant selectable marker in N. crassa transformation.

Cloning and characterization of the gene for beta-tubulin from a benomyl-resistant mutant of Neurospora crassa and its use as a dominant selectable marker

1986 ◽  
Vol 6 (7) ◽  
pp. 2452-2461
Author(s):  
M J Orbach ◽  
E B Porro ◽  
C Yanofsky
Keyword(s):  
Neurospora Crassa ◽  
Selectable Marker ◽  
Resistant Mutant ◽  
Intron Position ◽  
Coding Region ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Benomyl Resistance ◽  
Dominant Selectable Marker ◽  
Beta Tubulin Gene

We cloned the beta-tubulin gene of Neurospora crassa from a benomyl-resistant strain and determined its nucleotide sequence. The gene encodes a 447-residue protein which shows strong homology to other beta-tubulins. The coding region is interrupted by six introns, five of which are within the region coding for the first 54 amino acids of the protein. Intron position comparisons between the N. crassa gene and other fungal beta-tubulin genes reveal considerable positional conservation. The mutation responsible for benomyl resistance was determined; it caused a phenylalanine-to-tyrosine change at position 167. Codon usage in the beta-tubulin gene is biased, as has been observed for other abundantly expressed N. crassa genes such as am and the H3 and H4 histone genes. This bias results in pyrimidines in the third positions of 96% of the codons in codon families in which there is a choice between purines and pyrimidines in this position. Bias is also evident by the absence of 19 of the 61 sense codons. We demonstrated that benomyl resistance is due to the cloned beta-tubulin gene of strain Bml511(r)a and that this gene can be used as a dominant selectable marker in N. crassa transformation.

scholarly journals The highly divergent beta-tubulins of Aspergillus nidulans are functionally interchangeable.

1989 ◽  
Vol 109 (5) ◽  
pp. 2267-2274 ◽  
Author(s):  
G S May
Keyword(s):  
Aspergillus Nidulans ◽  
Nuclear Division ◽  
Selectable Marker ◽  
Wild Type ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Tubulin Genes ◽  
Internal Gene ◽  
Type Strains ◽  
Beta Tubulin Gene

An internal 1.4-kb Bst EII fragment was used to disrupt the benA gene and establish heterokaryons. The heterokaryons demonstrated that the molecular disruption of benA results in a recessive benA null mutation. Conidia from a heterokaryon swell and germinate but cannot undergo nuclear division and are thus inviable. A chimeric beta-tubulin gene was constructed with the benA promoter driving the tubC structural gene. This chimeric gene construction was placed on a plasmid containing a selectable marker for Aspergillus transformation and the gene disrupting fragment of benA. Integration of this plasmid at benA by the internal gene disrupting fragment of benA simultaneously disrupts the benA gene and replaces it with the chimeric beta-tubulin gene, rescuing the benA null generated by the integration. Strains generated by this procedure contain only tubC beta-tubulin for all beta-tubulin functions. Strains having only tubC beta-tubulin are viable and exhibit no detectable microtubule dysfunction though they are more sensitive than wild-type strains to the antimicrotubule drug benomyl. It is concluded that the two beta-tubulin genes of Aspergillus nidulans, though highly divergent, are interchangeable.

scholarly journals Nucleotide sequence analysis of beta tubulin gene in a wide range of dermatophytes

2014 ◽  
Vol 52 (7) ◽  
pp. 674-688 ◽  
Author(s):  
Ali Rezaei-Matehkolaei ◽  
Hossein Mirhendi ◽  
Koichi Makimura ◽  
G. Sybren de Hoog ◽  
Kazuo Satoh ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Nucleotide Sequence Analysis ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Wide Range ◽  
Beta Tubulin Gene

The beta tubulin gene of Eimeria tenella

1996 ◽  
Vol 76 (1-2) ◽  
pp. 315-319 ◽  
Author(s):  
Guan Zhu ◽  
Janet S. Keithly
Keyword(s):  
Eimeria Tenella ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Beta Tubulin Gene

Mutational analysis of the class i beta-tubulin gene in renal cell carcinoma (RCC)

2003 ◽  
Vol 2 (6) ◽  
pp. 66
Author(s):  
Roisean E Ferguson ◽  
Claire F Taylor ◽  
Anthea J Stanley ◽  
Roger M Phillips ◽  
Adrian D Joyce ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Mutational Analysis ◽  
Class I ◽  
Tubulin Gene ◽  
Beta Tubulin ◽  
Beta Tubulin Gene
Sign in / Sign up

Export Citation Format

Share Document