Embryogenesis and plant regeneration from tissue culture of Canada wildrye, Elymus canadensis L.

1989 ◽  
Vol 8 (5) ◽  
Author(s):  
C.H. Park ◽  
P.D. Walton
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Elymus Canadensis

Plant regeneration and chromosome instability in tissue culture of Elymus canadensis × E. trachycaulus F1 hybrid

Genome ◽  
1992 ◽  
Vol 35 (1) ◽  
pp. 88-91 ◽  
Author(s):  
P. S. Kumar ◽  
P. D. Walton
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Chromosome Number ◽  
Chromosome Instability ◽  
Ms Medium ◽  
Immature Inflorescence ◽  
F1 Hybrid ◽  
Regenerated Plants ◽  
Callus Initiation ◽  
Elymus Canadensis

Callus was induced from pieces of immature inflorescence of a sterile F1 hybrid (2n = 4x = 28) between Elymus canadensis and E. trachycaulus on MS medium supplemented with 2 mg/L of 2,4-D. Calli were of two types: compact or soft. Regeneration occurred predominantly from the compact callus. Although some plantlets were obtained on the callus initiation medium, the frequency of regeneration increased considerably when the callus was transferred to an auxin-free MS medium. Deliberate aging of callus induced cytological instability and variation in chromosome number of the regenerants. Five of the 43 regenerated plants had deviant chromosome numbers, including an octoploid (2n = 8x = 56). Chromosome pairing in the octoploid plant suggests that it originated through chromosome doubling.Key words: callus culture, variation, chromosome number.

scholarly journals Phloroglucinol Mediated Plant Regeneration of Ornithogalum dubium as the Sole “Hormone-Like Supplement” in Plant Tissue Culture Long-Term Experiments

Plants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 929
Author(s):  
Carloalberto Petti
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Callus Formation ◽  
Total Phenolic ◽  
Naturally Occurring ◽  
Long Term Experiments ◽  
Direct Embryogenesis ◽  
The Media ◽  
Synthetic Hormones

Tissue culture is an essential requirement in plant science to preserve genetic resources and to expand naturally occurring germplasm. A variety of naturally occurring and synthetic hormones are available to induce the processes of dedifferentiation and redifferentiation. Not all plant material is susceptible to tissue culture, and often complex media and hormone requirements are needed to achieve successful plant propagations. The availability of new hormones or chemicals acting as hormones are critical to the expansion of tissue culture potentials. Phloroglucinol has been shown to have certain hormone-like properties in a variety of studies. Ornithogalum dubium, an important geophyte species, was used to characterise the potential of phloroglucinol as the sole plant-like hormone in a tissue culture experiment. Tissue culture, plant regeneration, total phenolic and genetic variability were established by applying a variety of methods throughout long-term experiments. Phloroglucinol did induce callus formation and plant regeneration when used as the sole supplement in the media at a rate of 37%, thus demonstrating auxin/cytokines-like properties. Callus formation was of 3 types, friable and cellular, hard and compact, and a mixture of the two. The important finding was that direct somatogenesis did occur albeit more frequently on younger tissue, whereby rates of induction were up to 52%. It is concluded that phloroglucinol acts as a “hormone-like” molecule and can trigger direct embryogenesis without callus formation.

Study on Tissue Culture of Sweet Broad Pea

2014 ◽  
Vol 644-650 ◽  
pp. 5407-5410
Author(s):  
Hui Fang Chi
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Culture Medium ◽  
Callus Formation ◽  
Formation Rate ◽  
Adventitious Bud ◽  
Experimental Basis ◽  
Research Results

s. The cotyledons, Internodes, leaves and stems of sweet broad pea were studied on tissue culture. Research results show that: The ability of different explants for callus formation and adventitious bud differentiation in different culture medium is different. The callus formation rate and sprouting rate of Internodes is significantly higher than other explants, which is a ideal material for tissue culture. The callus formation rate of Internodes was 100% in MS +BA1.0 mg/L+NAA 1.0 mg/L and MS+ 2, 4-D 0.5 mg/L; The bud differentiation is best at the medium of MS+ 6-BA 2 mg/L, which reached 86.7%; the rooting rate was 83.3% at the medium of MS+ NAA 3mg/L. The study provides a experimental basis for further study on the plant regeneration in the sweet broad pea.

Induction of Plant Regeneration in Wheat Tissue Culture

1981 ◽  
Vol 176 (3) ◽  
pp. 236-243 ◽  
Author(s):  
Galina N. Yurkova ◽  
Boris A. Levenko ◽  
Oleg V. Novozhilov
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Wheat Tissue

Optimisation of Tissue Culture Conditions for Transformation Studies Using Immature Embryos of Australian Barley Cultivars

1995 ◽  
Vol 43 (5) ◽  
pp. 499 ◽  
Author(s):  
AM Walmsley ◽  
RJ Henry ◽  
RG Birch
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Culture Medium ◽  
Immature Embryo ◽  
Culture Conditions ◽  
Medium Composition ◽  
Immature Embryos ◽  
Systematic Testing ◽  
Barley Cultivars ◽  
Key Variables

Eight Australian barley cultivars were tested for efficiency of embryonic callus initiation and plant regeneration, from immature embryo explants in tissue culture. Optimisation of tissue culture conditions was performed for cultivars Bandulla, Clipper, Schooner and Tallon in an attempt to increase regeneration frequencies to levels suitable for genetic engineering of barley. Variables tested were 2,4-D concentration, salt composition, carbon source and immature embryo explant. Optimal culture medium composition varied between cultivars. Shoot regeneration rates from culture of isolated scutellar tissues were low for all four cultivars. Halved, immature embryos produced most shoots for cultivars Clipper, Schooner and Tallon, whereas Bandulla performed best with entire immature embryo explants. Clipper (a malting barley) and Bandulla (a feed barley) are suggested as model Australian cultivars for transformation studies. Immature embryos of Bandulla produced an average of 5.3 shoots and Clipper 10.1 shoots per embryo under optimal conditions. Our results show that rates of somatic embryo and plant regeneration sufficient for use in transformation studies can be achieved for diverse Australian Barley cultivars, through systematic testing of a range of key variables including explant type and medium composition.

Plant Regeneration in Wheat Tissue Culture

1982 ◽  
Vol 177 (4-5) ◽  
pp. 337-344 ◽  
Author(s):  
Galina N. Yurkova ◽  
Boris A. Levenko ◽  
Oleg V. Novozhilov
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Wheat Tissue
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