Plant regeneration and chromosome instability in tissue culture of Elymus canadensis × E. trachycaulus F1 hybrid

Genome ◽  
1992 ◽  
Vol 35 (1) ◽  
pp. 88-91 ◽  
Author(s):  
P. S. Kumar ◽  
P. D. Walton
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Chromosome Number ◽  
Chromosome Instability ◽  
Ms Medium ◽  
Immature Inflorescence ◽  
F1 Hybrid ◽  
Regenerated Plants ◽  
Callus Initiation ◽  
Elymus Canadensis

Callus was induced from pieces of immature inflorescence of a sterile F1 hybrid (2n = 4x = 28) between Elymus canadensis and E. trachycaulus on MS medium supplemented with 2 mg/L of 2,4-D. Calli were of two types: compact or soft. Regeneration occurred predominantly from the compact callus. Although some plantlets were obtained on the callus initiation medium, the frequency of regeneration increased considerably when the callus was transferred to an auxin-free MS medium. Deliberate aging of callus induced cytological instability and variation in chromosome number of the regenerants. Five of the 43 regenerated plants had deviant chromosome numbers, including an octoploid (2n = 8x = 56). Chromosome pairing in the octoploid plant suggests that it originated through chromosome doubling.Key words: callus culture, variation, chromosome number.

scholarly journals Somaclonal variation in triticale (×Triticosecale Wittmack) cv. Carman

1985 ◽  
Vol 27 (2) ◽  
pp. 151-157 ◽  
Author(s):  
M. C. Jordan ◽  
E. N. Larter
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Somaclonal Variation ◽  
Chromosomal Instability ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Regenerated Plants ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Triticosecale Wittmack ◽  
Kernel Protein

Callus was initiated from 15-day-old embryos of 'Carman' triticale (×Triticosecale Wittmack) cultured on 2,4-dichlorophenoxyacetic acid supplemented Murashige and Skoog (MS) medium. For plant regeneration, the calli were subcultured every 4 weeks on MS media with no added hormones. The original euploid (2n = 42) regenerated plants and their progeny were examined for several traits. Considerable variation for all measured traits was observed among the regenerated plants. Variability was greatest for spike length, fertility, and plant height. Two second-generation plants were found to have a significant increase in percent kernel protein relative to 'Carman' controls. Electrophoretic studies showed that all regenerated plants of both generations had the same prolamin banding pattern as 'Carman' triticale but variation existed in the intensity of the bands. This was especially true for the bands encoded for by the rye genome. One genotype, viz. R13, exhibited extreme chromosomal instability resulting in the occurrence of both rye and wheat univalents as observed at metaphase I.Key words: somaclonal variation, triticale, tissue culture, plant regeneration.

scholarly journals Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra

2008 ◽  
Vol 43 (10) ◽  
pp. 1325-1330 ◽  
Author(s):  
Lucymeire Souza Morais-Lino ◽  
Janay Almeida dos Santos-Serejo ◽  
Sebastião de Oliveira e Silva ◽  
José Raniere Ferreira de Santana ◽  
Adilson Kenji Kobayashi
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Somatic Embryos ◽  
Culture Media ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Male Flowers

The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP.

scholarly journals Micropropagation of Anubias barteri var. Nana from Shoot Tip Culture and the Analysis of Ploidy Stability

2012 ◽  
Vol 40 (2) ◽  
pp. 148 ◽  
Author(s):  
Kantamaht KANCHANAPOOM ◽  
Panyaros CHUNUI ◽  
Kamnoon KANCHANAPOOM
Keyword(s):  
Plant Regeneration ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Tip Culture ◽  
Ploidy Stability ◽  
Flow Cytometric ◽  
Regenerated Plants ◽  
Mother Plants

Plant regeneration of Anubias barteri var. Nana was achieved through organogenesis in shoot tip cultures. Multiple shoots were induced from cultured shoot tips on a modified MS (Murashige and Skoog, 1962) medium supplemented with BA and kinetin. The maximum green shoot numbers were best obtained on MS medium containing 3 mg/L BA with 5 shoots. Rooting in all regenerated shoots was promoted on MS medium devoid of plant growth regulators or kinetin singly. Acclimatization and survival when transferred to field conditions were shown to be 100% in the regenerated plants. Cytological and flow cytometric analyses of the mother plants and in vitro grown plants derived from 5 years old cultures showed no differences in ploidy level, they were all diploid (2n = 2x = 48) with a 2C peak indicating that ploidy alteration did not occur.

scholarly journals Effect of plant growth regulators on plant regeneration of hyacinth bean (Lablab purpureus (L.) Sweet) through nodal explants

2019 ◽  
Vol 44 (1) ◽  
pp. 27-42
Author(s):  
MT Islam ◽  
MS Haque ◽  
S Rahman ◽  
MR Molla ◽  
ERJ Keller
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Nodal Explants ◽  
Ms Medium ◽  
Lablab Purpureus ◽  
Shoot Initiation ◽  
Callus Initiation ◽  
Hyacinth Bean ◽  
Callus Size ◽  
Node Culture

Nodal explants of three accessions namely BD-101, BD-122 and BD-8001 of hyacinth bean (Lablab purpureus (L.) Sweet) were cultured for four weeks on MS medium supplemented with different concentrations and combinations of NAA (0, 0.1 mgl-1) and BAP (0.5, 1.0 and 2.5 mgl-1) for plant regeneration. The highest shoot initiation was observed in 0.5 mgl-1 BAP while the lowest shoot initiation was found in 2.5 mgl-1 BAP with 0.1 mgl-1 NAA. Earlier shoot initiation was exhibited in 0.5 mgl-1 BAP with 0.1 mgl-1 NAA. The highest number of leaflets and higher shoot lengths were observed in MS medium. Comparatively higher number of shoots was found in BAP (0.5-2.5 mgl-1). The highest percentage of callus was initiated in medium supplemented with 1.0 mgl-1 BAP. Earlier callus initiation and larger callus size were found in combination of BAP (0.5-2.5 mgl-1) with 0.1 mgl-1 NAA. BD-122 cultured in MS medium was found superior for shoot regeneration through node culture. Four different concentrations of IBA (0.1-1.5 mgl-1) were used for rooting. The highest percentage (86.67 %) of rooting was found in MS medium containing 0.1 mgl-1 IBA at four weeks. Rooting frequency decreased with the increasing concentration of IBA. The accession BD-8001 had 99.60 % rooting in 0.5 mgl-1 IBA. The highest number of longest roots was exhibited in 0.1 mgl-1 IBA. The regeneration protocol developed from nodal explants has applicability in improvement of hyacinth bean. Bangladesh J. Agril. Res. 44(1): 27-42, March 2019

scholarly journals Comparison of regeneration potentials in tissue cultures of primitive and cultivated tomato species (Lycopersicon sp.)

2014 ◽  
Vol 65 (1-2) ◽  
pp. 53-56 ◽  
Author(s):  
M. Lech ◽  
K. Miczyński ◽  
A. Pindel
Keyword(s):  
Tissue Culture ◽  
Ms Medium ◽  
Tissue Cultures ◽  
Lycopersicon Peruvianum ◽  
Wild Tomato ◽  
Wild Tomato Species ◽  
Tomato Species ◽  
Regenerated Plants ◽  
Modified Ms Medium ◽  
Tomato Cultivars

Regeneration capacities of two tomato cultivars: Potentat and Rutgers, and of three accessions of wild tomato species: <em>Lycopersicon peruvianum</em> PI 128650, <em>L. peruvianum var. dentatum</em> PI 128655 and <em>L. glandulosum</em> were studied using an universal medium suitable for regeneration of those plants from leaf pieces in tissue culture. Fragments of leaf blades were taken from plants raised in greenhouse conditions and placed on a modified MS medium containing 0.3 mg/l IAA and 3.0 mg/l BAP solidified with 1% agar. The explants were transferred every 4-5 weeks on fresh medium of the same composition. It was shown that all the three primitive tomato species revealed much higher multiplication coefficients than the two cultivars. Appropriate values were: 11 - for <em>L. glandulosum</em>, 8 - for <em>L. peruvianum</em>, 7 - for <em>L. peruvianum var. dentatum</em>, 4 - for <em>L. esculentum</em> cv. Potentat and 2 - cv. Rutgers. Completely regenerated plants were obtained from all the tested species, but organogenesis occurred almost two weeks earlier in wild tomatoes than in the culitivated varieties of <em>L. esculentum</em>.

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Physalis minima L. - a Perennial Medicinal Herb

2010 ◽  
Vol 44 (4) ◽  
pp. 453-456 ◽  
Author(s):  
Farhana Afroz ◽  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
Nadira Begum ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Half Strength ◽  
Shoot Tip Explants

The present study describes a protocol for high frequency plant regeneration of Physalis minima. Shoots were induced by culturing nodal segments and shoot tips from 15 day old seedlings. About 29 and 32 shoots were found to be induced from nodal segment and shoot tip explants, respectively, cultured on MS medium supplemented with 1.0 mg/l BAP. When shoots were subcultured on the fresh medium with same component as mentioned above, the shoots were elongated. Shoots rooted well when they were excised individually and implanted on half-strength MS medium with 0.3 mg/l NAA, where 98% shoots rooted within 12-15 days. In vitro grown plantlets with strong root system were successfully established in normal room temperature for seven days before transplanting in pots where they were reared for three weeks through successive acclimatization. The regenerated plants were successfully transferred to the soil with 90% survival rate. Key words: Physalis minima; Medicinal plant; Shoot proliferation; Micropropagation; Regeneration DOI: 10.3329/bjsir.v44i4.4597 Bangladesh J. Sci. Ind. Res. 44(4), 453-456, 2009

scholarly journals A Valued Medicinal Plant - Chitrak (Plumbago zeylanica Linn.) : Successful Plant Regeneration Through Various Explants and Field Performance

1970 ◽  
Vol 16 (2) ◽  
pp. 77-84 ◽  
Author(s):  
Binita B Chaplot ◽  
Ashok M Dave ◽  
Yogesh T Jasrai
Keyword(s):  
Plant Regeneration ◽  
Medicinal Plant ◽  
Morphological Change ◽  
Field Performance ◽  
Multiple Shoots ◽  
Ms Medium ◽  
Plant Propagation ◽  
Plumbago Zeylanica ◽  
Adenine Sulphate ◽  
Regenerated Plants

Protocols for plant propagation through axillary bud proliferation and organo-genesis were established for Chitrak - Plumbago zeylanica Linn. (Plumbaginaceae). MS medium with 4.4 mg/l BA and 1.4 mg/l IAA elicited the maximum number of shoots (12 multiple shoots) from nodal explants. Leaf based callus differentiated into more than 30 shoots on MS with 160 mg/l adenine sulphate. The regenerated shoots were rooted on MS with 1.2 mg/l IBA within ten days. Almost, 96% of the rooted shoots survived hardening when transferred to the field. The regenerated plants did not show any morphological change and variation in levels of secondary metabolite when compared with the mother stock.Key words: Medicinal plant, Regeneration, Field performanceDOI = 10.3329/ptcb.v16i2.1108Plant Tissue Cult. & Biotech. 16(2): 77-84, 2006 (December)

scholarly journals Effect of Plant Growth Regulators on Somatic Embryogenesis and Plantlet Development of Turkey Berry (Solanum torvum SW)

2021 ◽  
pp. 1-8
Author(s):  
Ghan Singh Maloth ◽  
Rajinikanth Marka ◽  
Rama Swamy Nanna
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Direct Somatic Embryogenesis ◽  
Ms Medium ◽  
Solanum Torvum ◽  
Plantlet Formation ◽  
Regenerated Plants

In the present study it was reported on direct somatic embryogenesis and plant regeneration from cotyledon and leaf explants of Turkey berry/pea egg plant (Solanum torvum SW), a medicinally important plant. Somatic embryogenesis has several advantages over other routes of in vitro plant regeneration. Somatic embryogenesis was induced directly from cotyledon and leaf explants on MS medium fortified with BAP (0.5 mg/L)+NAA (0.5-6.0 mg/L). High percentage of somatic embryogenesis (90%), maximum number of somatic embryos formation (62±0.18)  along with high percentage (76%) conversion of somatic embryos into bipolar embryos was observed on cotyledon explants in 0.5 mg/L BAP+2.5 mg/L NAA. At the same concentration of BAP (0.5 mg/L)+NAA (2.5 mg/L) also resulted  on the maximum percentage of somatic embryogenesis (92%), the highest number of somatic embryos formation (88±0.15) and the highest percentage (76%) of somatic embryos conversion into bipolar embryos in leaf explants. A mixture of globular, heart and torpedo-shaped embryos were germinated on MS medium supplemented with 0.5 mg/L IAA+1.0-4.0 mg/L BAP. Maximum germination frequency (75±0.14) of somatic embryos and plantlet formation was found in 0.5 mg/L IAA+2.0 mg/L BAP, but they didn’t germinate on ½ MSO and MSO media. The survival rate of regenerated plants after field transfer was recorded to be 75%. These regenerated plants were found morphologically similar to donor plants. The present protocol can be used for conservation of the species and also for genetic transformation experiments in S. torvum.

scholarly journals Use an Organic Biostimulant (Vermicompost Tea) For Enhancement In Vıtro Callus Growth in Sainfoin (Onobrychis viciifolia Scop.)

2019 ◽  
Vol 7 (2) ◽  
pp. 214
Author(s):  
Ramazan Beyaz ◽  
Fevziye Şüheyda Hepşen Türkay
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Plant Growth ◽  
Physiological Responses ◽  
Callus Growth ◽  
Stem Explants ◽  
Onobrychis Viciifolia ◽  
Callus Initiation ◽  
Vermicompost Tea

The health and vitality of callus growth is one of the prerequisites for the success of further in vitro studies. This study investigated the efficiency of different percentage (0%, 10%, 20%, 30%, and 40%) of vermicompost tea as an organic substance on in vitro callus growth in sainfoin. Morpho-physiological responses of calli to vermicompost tea measured under in vitro conditions. As a result of this investigation, a combination of plant growth regulators (4 mg/l BAP and 0.5 mg/l NAA) with 20% of vermicompost tea causing significant callus initiation and growth in sainfoin stem explants. Under the light of these scientific findings, vermicompost tea might be used as an organic bio stimulant for efficient callus growth and complementary to commercial chemical hormones in sainfoin. This research is important due to it can contribute positively to the plant species that are difficult in terms of callus growth and plant regeneration in tissue culture.

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