Wright-Giemsa Staining Method

2020 ◽  
Author(s):  
Keyword(s):  
Staining Method ◽  
Giemsa Staining

Investigation of Transmissible Venereal Tumor in Male Dogs by Cytological Examination

2020 ◽  
Author(s):  
C. Cagri Cingi ◽  
Deniz Yeni ◽  
Ebubekir Yazici ◽  
Tugba Akbas Cine ◽  
Mehmet Ucar
Keyword(s):  
Tumor Cells ◽  
Staining Method ◽  
Early Time ◽  
Clinical Findings ◽  
Giemsa Staining ◽  
Caudal Part ◽  
Cytological Examination ◽  
Tumor Mass ◽  
Intermediate Density

Transmissible Venereal Tumor (TVT) is one of the most uncontrolled spreading tumor via mating between dogs observed in both sexes. The clinical findings are less remarkable and are usually located in the caudal part of the penis and may include preputial discharge, licking of the region, dysuria, phimosis or paraphimosis (occasionally). In this study, the incidence of TVT and the relations of tumor with some factors were investigated in 145 male dogs, in Eskiºehir, Turkey. TVT’s diagnosis was based on location of the tumor mass and mainly exfoliative cytological findings. Smears of caudal part of the penis were painted with Giemsa staining method. The cells in the smears were identified as typical transmissible venereal tumor cells, polymorphnuclear leukocytes (PMN), erythrocyte, parabasal, intermediate, nucleated and anuclear superficial cells. In exfoliative cytological examinations, TVT cells were observed in the smears of 17 dogs (11,72 %), but only four of them (2,76 %) had TVT lesions clinically. Thirteen (8,97 %) of dogs had TVT cells but not having TVT lesions . It was found that the ages, weights and breeds of dogs had no effect on the TVT lesions and TVT cells. TVT positive animals were determined to have more erythrocyte and intermediate density than negatives and no association with other cells were found. PMNs were significantly different and high in the TVT cell positive cases compared to the negatives. In dogs with negative TVT cells, parabasal, intermediate, nuclear/anuclear superficial cells were more than those positives. As a result, the male dogs having no lesions could be infected with TVT. Dogs should be examined not only clinically, but also cytologically, to determine whether TVT cells are present. This method can be an easy way to find and treat TVT lesion-free but infected dogs at an early time.

First Molecular Evidence of Theileria Lestoquardi in Small Ruminants in Northern Iran

2021 ◽  
Author(s):  
Seyed Mousa Motavalli Haghi ◽  
Mahdi Fakhar ◽  
Mitra Sharbatkhori ◽  
Abdol Sattar Paghe
Keyword(s):  
Staining Method ◽  
Small Ruminants ◽  
Molecular Study ◽  
Common Species ◽  
Giemsa Staining ◽  
Molecular Evidence ◽  
Northern Iran ◽  
Theileria Lestoquardi ◽  
High Prevalence ◽  
Peripheral Blood Smears

Abstract Ovine theileriosis as a critical agent in small ruminant production, can cause lethal infections. Different species of Theileria have been reported in various parts of the world, and each species causes different diseases in the host. This is the first molecular study to investigate the prevalence of ovine theileriosis and identify the dominant Theileria species in northern Iran. A number of 220 small ruminants, including sheep and goats, were randomly sampled from 22 flocks. Peripheral blood smears are stained by the Giemsa staining method. As well as for species identification, all samples were examined by molecular method. From 220 samples, 160 and 60 were sheep and goat, respectively. By the Giemsa staining method, Theileria parasite was observed in 20 (9%) samples. But by polymerase chain reaction (PCR) method, 30 (13.6%) samples were positive for Theileria species. Theileria lestoquardi was the most common species found in these animals. The high prevalence of theileriosis in small ruminants demonstrates the emergence of ovine theileriosis in Mazandaran and Golestan provinces in northern Iran.

Chromosome morphometric analysis of Indian cultivars of Lens culinaris Medik. using EMA based Giemsa staining method

Caryologia ◽  
2017 ◽  
Vol 70 (3) ◽  
pp. 270-283 ◽  
Author(s):  
Timir Baran Jha ◽  
Partha Sarathi Saha ◽  
Mousumi Adak ◽  
Sumita Jha ◽  
Partha Roy
Keyword(s):  
Morphometric Analysis ◽  
Lens Culinaris ◽  
Staining Method ◽  
Giemsa Staining

Searching chromosomal landmarks in Indian lentils through EMA-based Giemsa staining method

PROTOPLASMA ◽  
2015 ◽  
Vol 253 (5) ◽  
pp. 1223-1231 ◽  
Author(s):  
Timir Baran Jha ◽  
Mihir Halder
Keyword(s):  
Staining Method ◽  
Giemsa Staining

scholarly journals The Acrosome Integrity Examination of Post-thawed Spermatozoa of Several Ongole Grade Bull in Indonesia Using Giemsa Staining Method

2020 ◽  
Vol 478 ◽  
pp. 012042
Author(s):  
K D Prihantoko ◽  
F Yuliastuti ◽  
H Haniarti ◽  
A Kusumawati ◽  
D T Widayati ◽  
...  
Keyword(s):  
Staining Method ◽  
Giemsa Staining ◽  
Acrosome Integrity
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