Production of terpenes by differentiated shoot cultures of Mentha citrata transformed with Agrobacterium tumefaciens T37

1990 ◽  
Vol 8 (10) ◽  
pp. 601-604 ◽  
Author(s):  
Andrew Spencer ◽  
John D. Hamill ◽  
Michael J. C. Rhodes
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Shoot Cultures ◽  
Mentha Citrata

Growth kinetics and stoichiometry of Mentha citrata shooty teratomas transformed by Agrobacterium tumefaciens

1995 ◽  
Vol 17 (4) ◽  
pp. 427-432 ◽  
Author(s):  
M. Ahkam Subroto ◽  
John D. Hamill ◽  
Pauline M. Doran
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Growth Kinetics ◽  
Mentha Citrata

scholarly journals Growth and In Vitro Propagation of Peach Plants Transformed with the Shooty Mutant Strain of Agrobacterium tumefaciens

HortScience ◽  
1998 ◽  
Vol 33 (5) ◽  
pp. 897-899 ◽  
Author(s):  
F.A. Hammerschlag ◽  
A.C. Smigocki
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Mutant Strain ◽  
In Vitro Propagation ◽  
Prunus Persica ◽  
Shoot Formation ◽  
Fresh Mass ◽  
Axillary Shoot ◽  
Shoot Cultures ◽  
Cytokinin Biosynthesis

Peach [Prunus persica (L.) Batsch] plants #94-1, #99-1, and #40-1, carrying a cytokinin biosynthesis (ipt) gene following transformation with the shooty mutant strain of Agrobacterium tumefaciens, were evaluated for altered growth habit and axillary shoot formation, both in vitro and in the greenhouse. After 9 weeks of in vitro propagation on four different levels of 6-benzyladenine (BA), only transformant #99-1 exhibited significantly greater axillary shoot formation (on 10 μm BA), and significantly greater fresh mass (on 3,10, and 30 μm BA) than the control #RG-3. Tolerance to a supra-optimal (30 μm) concentration of BA was indicated by fresh mass increases for #99-1 shoot cultures. Delayed senescence on 0 μm BA was exhibited by 87% of the transformants, but by only 12% of the control plants. Greenhouse-grown #99-1 and #40-1 were significantly shorter than #RG-3 plants at 6 weeks and at 1 year, but only #40-1 exhibited significantly greater branching than the controls. Chemical names used: 6-benzyladenine (BA); isopentenyl transferase (ipt).

scholarly journals TRANSFORMATION OF ORANGEMINT (MENTHA CITRATA, EHRH) WITH AGROBACTERIUM TUMEFACIENS

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1165c-1165 ◽  
Author(s):  
Christine Berry ◽  
J. Van Eck ◽  
S. Kitto ◽  
A. Smigocki
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Blot Analysis ◽  
Virulent Strain ◽  
Ti Plasmid ◽  
Slot Blot ◽  
Mentha Citrata ◽  
Slot Blot Analysis ◽  
Callus Lines ◽  
Leaf Disks

Orangemint leaf disks were infected with three strains of Agrobacterium tumefaciens: A281, a hyper-virulent strain containing plasmid pTiBo542; C58, a strain containing nopaline Ti plasmid pTiC58; and A136, a derivative of C58 lacking the nopaline Ti plasmid. After a 24 or 48 hr cocultivation, leaf disks were placed on a medium containing MS salts and vitamins, 2% sucrose, and 200 μgm l-1 cefotaxime. Callus formed only on those leaf disks infected with A281. Five callus lines (R-12, -71, -73, -81, -83) were putatively transformed based on succinopine production. Definitive transformations were confirmed via DNA slot blot analysis. All callus lines assayed (R-11. -12, -41, -73, -83) hybridized to a 7.7 KB fragment from the T-DNA region.

Genetic transformation of Ginkgo biloba by Agrobacterium tumefaciens

2000 ◽  
Vol 108 (4) ◽  
pp. 413-419
Author(s):  
Patricia Dupré ◽  
Jerôme Lacoux ◽  
Godfrey Neutelings ◽  
Dominique Mattar-Laurain ◽  
Marc-André Fliniaux ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Ginkgo Biloba
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