STUDI REGENERASI IN VITRO DAN TRANSFORMASI GENETIK GEN COAT PROTEIN DAN BETA COMPONENT GEMINIVIRUS MELALUI Agrobacterium tumefaciens PADA TIGA GENOTIPE CABAI MERAH (Capsicum annuum L.)

2015 ◽  
Author(s):  
RENFIYENI RENFIYENI
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Coat Protein ◽  
Capsicum Annuum ◽  
Capsicum Annuum L

Micropropagation of Pepper (Capsicum annuum L.) Through in vitro Direct Organogenesis Organogenesis

2010 ◽  
Vol 3 (1) ◽  
pp. 38-45 ◽  
Author(s):  
Mahmoud Otroshy ◽  
Kosar Moradi ◽  
Mojtaba Khayam Nekouei ◽  
Paul C. Struik
Keyword(s):  
Capsicum Annuum ◽  
Direct Organogenesis ◽  
Capsicum Annuum L

scholarly journals Susceptibilidad de aislados de Xanthomonas campestris pv. vesicatoria a Streptomyces y extractos bioactivos de Ganoderma

2018 ◽  
Vol 27 (6) ◽  
pp. 30-39
Author(s):  
Loreto Robles-Hernández ◽  
Dámaris Leopoldina Ojeda-Barrios ◽  
Ana Cecilia González-Franco ◽  
Jared Hernández-Huerta ◽  
Nora Aideé Salas-Salazar ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Ganoderma Lucidum ◽  
Xanthomonas Campestris ◽  
Streptomyces Lydicus ◽  
Capsicum Annuum L

En el presente estudio, 10 aislados bacterianos de chile (Capsicum annuum L.) fueron identificados como Xanthomonas campestris pv. vesicatoria; se determinó su patogenicidad en invernadero y se evaluó su susceptibilidad a Streptomyces lydicus y extractos bioactivos de Ganoderma lucidum en condiciones in vitro y de invernadero, utilizando en cada caso un diseño completamente al azar. Todos los aislados causaron infección con una incidencia del 100% y variaciones en su severidad. Aunque todos los aislados fueron susceptibles a los tratamientos biológicos, los extractos bioactivos fueron superiores con un 100% de inhibición in vitro, así como en la reducción de infección foliar y población bacteriana en invernadero. Destacó una correlación fuerte entre población bacteriana e infección foliar (r = 0.9139**) en plantas inoculadas únicamente con el patógeno; en plantas tratadas adicionalmente con los biológicos, solo hubo una correlación débil en cada caso, sugiriendo su efecto benéfico en la supresión de X. campestris pv. vesicatoria.

scholarly journals Pengaruh Praperlakuan Dingin Antera terhadap Pembentukan Kalus Cabai Merah Keriting (Capsicum annuum L.) Pretreatment on the Callus Formation of Curly Red Chilli Pepper (Capsicum annuum L.)

2020 ◽  
Vol 4 (2) ◽  
pp. 94-105
Author(s):  
Nida Wafiqah Nabila M Solin ◽  
Dian Adriani ◽  
Zulfahmi Zulfahmi ◽  
Mokhamad Irfan ◽  
Rosmaina Rosmaina
Keyword(s):  
Anther Culture ◽  
Capsicum Annuum ◽  
Callus Formation ◽  
Chili Pepper ◽  
Haploid Plant ◽  
Cold Pretreatment ◽  
Compact Structure ◽  
Chilli Pepper ◽  
Capsicum Annuum L

The production of the double haploid plant in vitro through anther culture technique is a plant breeding technique used to obtain pure strain rapidly. A variety of pretreatment has been reported to induce callus and regenerate planlets efficiently. This study aims at describing the influence of cold anther pretreatment towards the callus formation of curly red chili pepper (Capsicum annuum L.). This research was conducted in the laboratory of Genetics and Breeding, Faculty of Agriculture and Animal Science, Universtas Islam Negeri Sultan Syarif Kasim Riau. The explants used are anther of local genotype of curly red chili pepper. The anthers are stored at low temperatures (4 °c) with different time intervals of 0, 24, 48 and 72 hours. The results showed that the percentage of highest callus formation was obtained at 24 and 72 hours length storage, ie 50%. Cold pretreatment of 72 hours anther storage results in a faster callus with a percentage of the highest yellowish white callus color of 17.65% and a compact structure. The cold pretreatment with 72 hours anther storage is the most optimal acceleration in the development stage of anther culture and induces te formation of curly red chili pepper (Capsicum annuum L.) local genotypes.

scholarly journals NADPH Oxidase (Rboh) Activity is Up Regulated during Sweet Pepper (Capsicum annuum L.) Fruit Ripening

Antioxidants ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 9 ◽  
Author(s):  
Ángela Chu-Puga ◽  
Salvador González-Gordo ◽  
Marta Rodríguez-Ruiz ◽  
José M. Palma ◽  
Francisco J. Corpas
Keyword(s):  
Oxidative Stress ◽  
Nadph Oxidase ◽  
Capsicum Annuum ◽  
Fruit Ripening ◽  
Biochemical Characterization ◽  
Sweet Pepper ◽  
Oxidative Stress Marker ◽  
Vitro Assay ◽  
Capsicum Annuum L

In plants, NADPH oxidase (NOX) is also known as a respiratory burst oxidase homolog (Rboh). This highly important enzyme, one of the main enzymatic sources of superoxide radicals (O2•−), is involved in the metabolism of reactive oxygen and nitrogen species (ROS and RNS), which is active in the non-climacteric pepper (Capsicum annuum L.) fruit. We used sweet pepper fruits at two ripening stages (green and red) to biochemically analyze the O2•−-generating Rboh activity and the number of isozymes during this physiological process. Malondialdehyde (MDA) content, an oxidative stress marker, was also assayed as an index of lipid peroxidation. In red fruits, MDA was observed to increase 2-fold accompanied by a 5.3-fold increase in total Rboh activity. Using in-gel assays of Rboh activity, we identified a total of seven CaRboh isozymes (I–VII) which were differentially modulated during ripening. CaRboh-III and CaRboh-I were the most prominent isozymes in green and red fruits, respectively. An in vitro assay showed that CaRboh activity is inhibited in the presence of nitric oxide (NO) donors, peroxynitrite (ONOO−) and glutathione (GSH), suggesting that CaRboh can undergo S-nitrosation, Tyr-nitration, and glutathionylation, respectively. In summary, this study provides a basic biochemical characterization of CaRboh activity in pepper fruits and indicates that this O2•−-generating Rboh is involved in nitro-oxidative stress associated with sweet pepper fruit ripening.

In vitro regeneration in chile pepper (Capsicum annuum L.) from ?half-seed explants?

1996 ◽  
Vol 20 (3) ◽  
pp. 287-293 ◽  
Author(s):  
Marla L. Binzel ◽  
N. Sankhla ◽  
S. Joshi ◽  
D. Sankhla
Keyword(s):  
Capsicum Annuum ◽  
In Vitro Regeneration ◽  
Chile Pepper ◽  
Capsicum Annuum L ◽  
Half Seed

Anthocyanins, Colour and Antioxidant Properties of Eggplant (Solanum melongena L.) and Violet Pepper (Capsicum annuum L.) Peel Extracts

2006 ◽  
Vol 61 (7-8) ◽  
pp. 527-535 ◽  
Author(s):  
Eva Sadilova ◽  
Florian C. Stintzing ◽  
Reinhold Carle
Keyword(s):  
Antioxidant Capacity ◽  
Capsicum Annuum ◽  
Nutritional Value ◽  
Antioxidant Properties ◽  
Solanum Melongena ◽  
In Vitro Assays ◽  
Capsicum Annuum L ◽  
Food Colour ◽  
Acetone Extracts

Acetone extracts from eggplant (Solanum melongena L.) and violet pepper (Capsicum annuum L.) peels both belonging to the Solanaceae plant family were characterized with respect to their anthocyanin profiles, colour qualities and antioxidant capacities. According to HPLC-DAD-MS3 analyses the major anthocyanin in eggplant was delphinidin-3-rutinoside, while the predominant pigment in violet pepper was assigned to delphinidin-3-transcoumaroylrutinoside- 5-glucoside. Since virtually all anthocyanins were delphinidin-based, the effect of acylation and glycosylation patterns on colour stability and antioxidant capacity could be assessed. Application of two in vitro-assays for antioxidant capacity assessment revealed that eggplant generally exhibited higher values compared to violet pepper which was ascribed to 3,5-diglycosylated structures predominating in the latter. The higher extent of acylation in violet pepper was reflected by a more purplish colour shade of the extracts, but did not translate into a higher stability against fading which again was attributed to additional glycosyl substitution at C5. These findings support the relevance of structurerelated activities of anthocyanins both for understanding food colour and their particular nutritional value.

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