Comparative greenhouse study of Eucalyptus grandis in vitro plantlets and half-sib seedlings, I. Net photosynthesis

1989 ◽  
Vol 8 (8) ◽  
pp. 497-499 ◽  
Author(s):  
E. I. Warrag ◽  
M. S. Lesney ◽  
D. L. Rockwood
Keyword(s):  
Eucalyptus Grandis ◽  
Net Photosynthesis ◽  
Greenhouse Study ◽  
In Vitro Plantlets

scholarly journals 897 PB 511 ACCLIMATIZATION AND SUBSEQUENT GAS EXCHANGE, WATER RELATIONS, AND GROWTH OF MICROCULTURED APPLE SHOOTS AFTER TRANSPLANTING

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 562d-562
Author(s):  
Juan C Díaz ◽  
Kenneth Shackel ◽  
Ellen Sutter
Keyword(s):  
Gas Exchange ◽  
Relative Water Content ◽  
Water Status ◽  
Net Photosynthesis ◽  
Plant Water ◽  
Ms Medium ◽  
In Vitro Plantlets ◽  
Relative Water ◽  
Survival And Growth

Apple plantlets were cultured in a MS medium with agar and transplanted to a soilless mix. Before transplanting, plantlets were acclimatized in a chamber where humidity was linearly decreased from 99% to 75% (21 C) over a period of four days. Gas exchange measurements were made at 95% RH (21 C) and at an irradiance of 350 μmol m-2 s-1. Leaf conductance (g1) was measured with a porometer and net photosynthesis (Pn) with an IRGA. At the end of the gas exchange measurements, shoot relative water content (RWC) was determined. The results showed that plant water status was an important factor for plant survival and growth after transplanting. Acclimatization before transplanting allowed the plant to maintain a higher RWC, probably because of a better stomatal control of transpiration. Such higher water status was associated with higher growth rates in acclimatized than in non-acclimatized plants. There was a positive correlation of RWC with both a and Pn. Transplanted plants had higher values of g1 and Pn compared to in vitro plantlets. Photosynthesis of in vitro plantlets was limited by both stomatal and nonstomatal factors.

Sodium nitroprusside promotes multiplication and regeneration of Malus hupehensis in vitro plantlets

2008 ◽  
Vol 96 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Xiaojiao Han ◽  
Hongqiang Yang ◽  
Kaixuan Duan ◽  
Xinrong Zhang ◽  
Haizhou Zhao ◽  
...  
Keyword(s):  
Sodium Nitroprusside ◽  
Malus Hupehensis ◽  
In Vitro Plantlets

scholarly journals Shoot Multiplication and Callus Induction of Labisia pumila var. alata as Influenced by Different Plant Growth Regulators Treatments and Its Polyphenolic Activities Compared with the Wild Plant

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3229
Author(s):  
Mat Yunus Najhah ◽  
Hawa Z. E. Jaafar ◽  
Jaafar Juju Nakasha ◽  
Mansor Hakiman
Keyword(s):  
Callus Induction ◽  
Antioxidant Activities ◽  
Shoot Multiplication ◽  
Wild Plants ◽  
Wild Plant ◽  
Total Phenolic ◽  
Nodal Segment ◽  
Ms Medium ◽  
In Vitro Plantlets

This study aims to investigate whether the in vitro-cultured L. pumila var. alata has higher antioxidant activity than its wild plant. An 8-week-old L. pumila var. alata nodal segment and leaf explants were cultured onto Murashige and Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, and 6-benzylaminopurine (BAP)) for shoot multiplication and auxins (2,4-dichlorophenoxyacetic acid (2,4-D) and picloram) for callus induction, respectively. The results showed that 2 mg/L zeatin produced the optimal results for shoot and leaf development, and 0.5 mg/L 2,4-D produced the highest callus induction results (60%). After this, 0.5 mg/L 2,4-D was combined with 0.25 mg/L cytokinins and supplemented to the MS medium. The optimal results for callus induction (100%) with yellowish to greenish and compact texture were obtained using 0.5 mg/L 2,4-D combined with 0.25 mg/L zeatin. Leaves obtained from in vitro plantlets and wild plants as well as callus were extracted and analyzed for their antioxidant activities (DPPH and FRAP methods) and polyphenolic properties (total flavonoid and total phenolic content). When compared with leaf extracts of in vitro plantlets and wild plants of L. pumila var. alata, the callus extract displayed significantly higher antioxidant activities and total phenolic and flavonoid content. Hence, callus culture potentially can be adapted for antioxidant and polyphenolic production to satisfy pharmaceutical and nutraceutical needs while conserving wild L. pumila var. alata.

scholarly journals Expression Level of Mature miR172 in Wild Type and StSUT4-Silenced Plants of Solanum tuberosum Is Sucrose-Dependent

2021 ◽  
Vol 22 (3) ◽  
pp. 1455
Author(s):  
Varsha Garg ◽  
Aleksandra Hackel ◽  
Christina Kühn
Keyword(s):  
Solanum Tuberosum ◽  
Wild Type ◽  
Potato Plants ◽  
Mature Leaves ◽  
In Vitro Plantlets ◽  
Long Time ◽  
Short Time ◽  
Cut Leaves

In potato plants, the phloem-mobile miR172 is involved in the sugar-dependent transmission of flower and tuber inducing signal transduction pathways and a clear link between solute transport and the induction of flowering and tuberization was demonstrated. The sucrose transporter StSUT4 seems to play an important role in the photoperiod-dependent triggering of both developmental processes, flowering and tuberization, and the phenotype of StSUT4-inhibited potato plants is reminiscent to miR172 overexpressing plants. The first aim of this study was the determination of the level of miR172 in sink and source leaves of StSUT4-silenced as well as StSUT4-overexpressing plants in comparison to Solanum tuberosum ssp. Andigena wild type plants. The second aim was to investigate the effect of sugars on the level of miRNA172 in whole cut leaves, as well as in whole in vitro plantlets that were supplemented with exogenous sugars. Experiments clearly show a sucrose-dependent induction of the level of mature miR172 in short time as well as long time experiments. A sucrose-dependent accumulation of miR172 was also measured in mature leaves of StSUT4-silenced plants where sucrose export is delayed and sucrose accumulates at the end of the light period.

scholarly journals Frequências de cortes em nove leguminosas forrageiras tropicais herbáceas cultivadas ao sol e sob plantação florestal

2015 ◽  
Vol 67 (3) ◽  
pp. 809-818 ◽  
Author(s):  
M.L.F. Nicodemo ◽  
F.H.D. Souza ◽  
J.R.M. Pezzopane ◽  
J.C.T. Mendes ◽  
P. Tholon ◽  
...  
Keyword(s):  
Eucalyptus Grandis

O objetivo deste trabalho foi estudar a produção e o valor nutritivo de nove espécies de leguminosas forrageiras herbáceas tropicais, cultivadas sob dois níveis de irradiação luminosa e seis frequências de corte na estação chuvosa, em Anhembi (SP), Brasil. Parcelas puras foram estabelecidas ao sol e sob plantação de Eucalyptus grandis, com 8 anos de idade, com 1.000 árvores/ha, que interceptou mais de 80% da radiação luminosa incidente. Avaliou-se a taxa de acúmulo de matéria seca, a proteína bruta e a digestibilidade in vitro da matéria seca em amostras de plantas colhidas em intervalos de 30, 60, 90 e 180 dias, com cortes adicionais aos 120 e 150 dias. Sob pleno sol, as leguminosas testadas toleraram cortes mais frequentes (intervalos de 30 - 90 dias), exceto o Lablab. Na sombra, o grau de tolerância variou conforme a espécie; em termos de produção de MS e de persistência, a Centrosema e a Puerária se destacaram dentre as demais leguminosas comparadas, quando os cortes foram realizados em intervalos de 60 dias, enquanto o Arachis e o Estilosantes foram os mais produtivos quando os cortes ocorreram em intervalos de 120 dias, mantendo o valor nutricional. O Alysicarpus e a Aeschynomene não persistiram sob o nível de sombreamento que prevaleceu nesse experimento.

scholarly journals IBA AND MICROCUTTING COLLECTIONS IN THE MICROPROPAGATION OF Eucalyptus spp HYBRID CLONES.1

2017 ◽  
Vol 41 (6) ◽  
Author(s):  
Ricardo Gallo ◽  
Aloisio Xavier ◽  
Luciana Coelho de Moura ◽  
Brener de Almeida Oliveira ◽  
Heloisa Rocha do Nascimento ◽  
...  
Keyword(s):  
Eucalyptus Grandis ◽  
Stem Diameter ◽  
Residual Effects ◽  
Seedling Height ◽  
Seedling Vigor ◽  
Eucalyptus Urophylla ◽  
Ex Vitro ◽  
Semisolid Medium ◽  
Hybrid Clones

ABSTRACT This study aims to evaluate the effect of IBA concentrations and microcuttings successive collections in the micropropagation of Eucalyptus grandis x E. urophylla and Eucalyptus urophylla x E. globulus clones. Clumps containing six to eight buds of clones established in vitro were transferred to a 250 mL glass flask in JADS semisolid medium. Successive collections were performed every 20 days for Eucalyptus grandis x E. urophylla clone and every 30 days for Eucalyptus urophylla x E. globulus clone. The following variables were evaluated under in vitro conditions: number of shoots > 0.5 cm, number of microcuttings > 2 cm, length of the longest microcutting, and shoots vigor. Under ex vitro conditions, in the greenhouse and shade house, the following variables were evaluated: seedling height, percentage of survival, stem diameter, percentage of root observed at the lower end of the tube, and seedling vigor. In full sun (ex vitro), the following variables were analyzed: seedling height, stem diameter, survival, number of roots, root volume, seedling vigor, and shoot and root dry matter. Good in vitro microcuttings productivity was observed over the successive collections. IBA levels were adjusted for each clone, ranging from 0.25 to 0.50 mg L-1 for Eucalyptus grandis x E. urophylla clone, and from 0.75 to 1.0 mg L-1 for Eucalyptus urophylla x E. globulus clone. IBA concentrations led to residual effects under ex vitro conditions, providing good rooting and survival for Eucalyptus grandis x E. urophylla and Eucalyptus urophylla x E. globulus clones at IBA concentrations between 0.25 and 0.50 mg L-1 and between 0.50 and 1.0 mg L-1, respectively.

scholarly journals ASSESSMENT OF GENETIC STABILITY OF MICROPROPAGATED Eucalyptus globulus Labill HYBRID CLONES BY MEANS OF FLOW CYTOMETRY AND MICROSATELLITES MARKERS

2017 ◽  
Vol 41 (1) ◽  
Author(s):  
Leandro Silva Oliveira ◽  
Aloisio Xavier ◽  
Wagner Campos Otoni ◽  
José Marcello Salabert Campos ◽  
Lyderson Facio Viccini ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Microsatellite Markers ◽  
Genetic Stability ◽  
Culture Medium ◽  
Eucalyptus Grandis ◽  
Genetic Fidelity ◽  
Shoot Multiplication ◽  
Micropropagated Plants ◽  
Hybrid Clones

ABSTRACT Flow cytometry and microsatellite markers were used to determine a genetic fidelity of micropropagated plants from the two Eucalyptus urophylla x E. globulus clones and a Eucalyptus grandis x E. globulus clone derived from adult material. Clones were repeatedly subcultured for 25 subcultures on MS medium supplemented with BA (2.22 µM) and ANA (0.05 µM) for in vitro shoot multiplication. The elongation was performed in MS culture medium supplemented with AIB (2.46 µM) and BA(0.22 µM). The ex vitro rooting and acclimatization phases were lead at the same time. The micropropagated clones showed genetic stability by flow cytometry and microsatellite markers. The results proved that micropropagation, for purposes of rejuvenation, can be a viable technique to generate genetically stable or identical E. globulus hybrid clones.

Exogenous sucrose can decrease in vitro photosynthesis but improve field survival and growth of coconut (Cocos nucifera L.) in vitro plantlets

2005 ◽  
Vol 41 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Gabriela Fuentes ◽  
Carlos Talavera ◽  
Carlos Oropeza ◽  
Yves Desjardins ◽  
Jorge M. Santamaria
Keyword(s):  
Cocos Nucifera ◽  
In Vitro Plantlets ◽  
Survival And Growth ◽  
Cocos Nucifera L
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