Exogenous sucrose can decrease in vitro photosynthesis but improve field survival and growth of coconut (Cocos nucifera L.) in vitro plantlets

2005 ◽  
Vol 41 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Gabriela Fuentes ◽  
Carlos Talavera ◽  
Carlos Oropeza ◽  
Yves Desjardins ◽  
Jorge M. Santamaria
Keyword(s):  
Cocos Nucifera ◽  
In Vitro Plantlets ◽  
Survival And Growth ◽  
Cocos Nucifera L

Subsitusi Fitohormon Dengan Air Kelapa (Cocos nucifera L.) pada Medium Vacin and Went Terhadap Pertumbuhan Eksplan Anggrek Dendrobium sp Secara in Vitro

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Wulan Dari Neng Gumiwang ◽  
Tintrim Rahayu ◽  
Ari Hayati
Keyword(s):  
Root Length ◽  
Cocos Nucifera ◽  
Water Concentration ◽  
Coconut Water ◽  
Culture Bottle ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Cocos Nucifera L

The purpose of this research is to determine the concentration of young coconut water that is appropriate for the growth of orchid plantlets (Dendrobium sp.) In vitro. This study used an experimental method, descriptive data analysis to compare several different concentrations of coconut water. The design of this study uses a completely randomized design (CRD). The treatments consist of 0% coconut water concentration (as a control), 15%, 30% and 60%. Each concentration was carried out 5 replications and each repetition consisted of 5 Dendrobium sp plantlets in each culture bottle conducted for 40 HST, for observing the root length carried out for 50 HST. The highest number of shoots and leaves were produced at the same concentration, namely 150 ml / L coconut water treatment (15% concentration) with an average of 2.8 shoots and the average number of leaves 10.8 leaves. The average number of roots and the longest root length was produced at a concentration of 600 ml / L coconut water (60% concentration) with an average of 6 roots, and the longest root length was 0.5 cm.Keywords: Young coconut water, (Cocos nucifera L.), Dendrobium sp., in vitro, growth.ABSTRAKTujuan penelitian ini ialah menentukan konsentrasi air kelapa muda yang tepat untuk pertumbuhan planlet anggrek (Dendrobium sp.) secara in vitro. Penelitian ini menggunakan metode eksperimen, analisis data secara deskriptif untuk membandingan beberapa konsentrasi air kelapa yang berbeda. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap (RAL). Perlakukan terdiri dari konsentrasi air kelapa 0 % (sebagai kontrol), 15% , 30% dan 60%. Masing-masing konsentrasi dilakukan 5 kali ulangan dan setiap ulangan terdiri dari 5 planlet Dendrobium sp dalam setiap botol kultur yang dilakukan selama 40 HST, untuk pengamatan panjang akar dilakukan selama 50 HST. Jumlah tunas dan jumlah daun terbanyak dihasilkan pada konsentrasi yang sama, yaitu perlakuan air kelapa 150 ml/L (konsentrasi 15%)  dengan rata-rata jumlah tunas terbanyak 2,8 tunas dan rata-rata jumlah daun terbanyak 10,8 helai daun. Rata-rata jumlah akar terbanyak dan panjang akar terpanjang dihasilkan pada konsentrasi air kelapa 600 ml/L (Konsentrasi 60%) dengan rata-rata jumlah akar terbanyak sebanyak 6 akar, dan rata-rata panjang akar terpanjang 0,5 cm.Kata kunci : Air kelapa Muda (Cocos nucifera L.), Dendrobium sp., in vitro, pertumbuhan 

scholarly journals UJI DAYA HAMBAT ASAP CAIR TEMPURUNG KELAPA (Cocos nucifera L.) TERHADAP PERTUMBUHAN Escherichia coli SECARA In Vitro

2021 ◽  
Vol 9 (2) ◽  
pp. 81
Author(s):  
Rachel Daniella Dinda Maria Lumban Tobing ◽  
Made Ria Defiani ◽  
Ni Made Susun Parwanayoni
Keyword(s):  
Escherichia Coli ◽  
Cocos Nucifera ◽  
E Coli ◽  
Cocos Nucifera L

Daging yang terkontaminasi bakteri berpotensi menimbulkan penyakit yang berbahaya apabila dikonsumsi manusia. Salah  satu  kuman  khususnya bakteri  yang  mencemari  daging  baik  yang  mentah  atau  daging dengan  proses  pematangan  yang  kurang  sempurna  adalah Escherichia coli. Oleh sebab itu, E. coli perlu diminimalisir dengan cara menghambat pertumbuhannya. Salah satu cara alami untuk menghambat pertumbuhan E. coli adalah dengan uji antibakteri menggunakan asap cair. Asap cair dapat diperoleh melalui proses pirolisis dari berbagai biomassa yang mengandung selulosa, hemiselulosa, dan lignin seperti pada tempurung kelapa (Cocos nucifera L.). Asap cair mengandung senyawa fenol dan asam yang bersifat antimikroba dan antioksidan. Penelitian ini menggunakan metode sumur difusi. Analisis data menggunakan Rancangan Acak Lengkap (RAL) dengan uji ragam (ANOVA) apabila data memiliki beda nyata pada taraf uji 5% (P?5) maka dilanjutjan uji Duncan. Asap cair tempurung kelapa mampu menghambat pertumbuhan E.coli yang ditandai dengan terbentuknya zona hambat. Konsentrasi asap cair tempurung kelapa yang efektif menghambat pertumbuhan E. coli adalah konsentrasi 50% dengan diameter zona hambat 16,66 mm dan MIC pada konsentrasi 10% dengan diameter zona hambat 9,33 mm. Hasil uji fitokimia asap cair tempurung kelapa positif mengandung senyawa fenol, flavonoid, triterpenoid, dan saponin serta kadar fenol 2,403% dan kadar saponin 5,50%.

scholarly journals Comparative DNA Analysis of Coconut (Cocos nucifera L.) palms with polyembryonic and monoembryonic origins

2020 ◽  
pp. 21-29
Author(s):  
Daisy Jane Toting ◽  
Tessie Nuñez ◽  
Dilberto Ferraren
Keyword(s):  
Dna Analysis ◽  
Cocos Nucifera ◽  
Polyacrylamide Gel Electrophoresis ◽  
The Philippines ◽  
Pcr Products ◽  
Planting Materials ◽  
Cocos Nucifera L ◽  
Dna Primers

Makapuno is a rare, high-value coconut in the Philippines known for its extraordinary thick gelatinous meat with various uses in the food industry. Homozygous makapuno embryos do not germinate in vivo so plantlets are produced in vitro. where one plantlet grows from an embryo. Rare cases of polyembryony were observed in makapuno hybrids developed bythe Visayas State University, Knowledge of the genetic control of polyembryony may be used to increase the production of planting materials of these rare coconut types. DNA analysis of two sets of twins (polyembryonic), three monoembryonic hybrid palms, and their monoembryonic parental cultivars Coconiño and tall makapuno was done using seven DNA primers to determine differences which may be associated with polyembryony in the hybrids. Polyacrylamide Gel Electrophoresis of PCR products showed DNA fragments amplified by primers CAC2 and CAC56 which are unique to the twins suggesting that polyembryony might have a genetic origin.

Lauric acid improves the growth of zygotic coconut (Cocos nucifera L.) embryos in vitro

2011 ◽  
Vol 106 (2) ◽  
pp. 317-327 ◽  
Author(s):  
Arturo López-Villalobos ◽  
Peter F. Dodds ◽  
Roland Hornung
Keyword(s):  
Lauric Acid ◽  
Cocos Nucifera ◽  
Cocos Nucifera L

Morphological and agronomical characteristics of coconut (Cocos nucifera L.) palms produced from in vitro cultured zygotic embryos

2013 ◽  
Vol 49 (5) ◽  
pp. 599-604 ◽  
Author(s):  
Yoboué Koffi ◽  
Oulo N’Nan-Alla ◽  
Jean-Louis Konan Konan ◽  
Bernard Malaurie ◽  
Florent Engelmann
Keyword(s):  
Cocos Nucifera ◽  
Zygotic Embryos ◽  
Cocos Nucifera L

scholarly journals EFFECT OF DIFFERENT BRANDS OF ACTIVATED CARBON ON GROWTH AND DEVELOPMENT OF COCONUT (Cocos Nucifera L) EMBRYOS IN VITRO

CORD ◽  
1998 ◽  
Vol 14 (02) ◽  
pp. 34
Author(s):  
Zosimo S. Bonaobra III ◽  
Erlinda P. Rillo ◽  
Osmundo D. Orense
Keyword(s):  
Growth And Development ◽  
Cocos Nucifera ◽  
Primary Root ◽  
Shoot Length ◽  
Zygotic Embryos ◽  
Percentage Germination ◽  
Significant Difference ◽  
Initial Culture ◽  
Cocos Nucifera L

The effect of different brands of activated charcoal [Merck GR (Art. 2186), Sigma acid washed (C‑4386), Sigma neutralized (C‑3790), and Duchefa neutralized (C‑1302)] on growth and development ofcoconut zygotic embryos in vitro was evaluated. Analysis of data noted after one month revealed that there was no significant difference on percentage germination, shoot length, and number ofprimary root of cv. Laguna Tall embryos cultured in Y3 liquid medium supplemented with 2.5gll AC of different brands. Length of primary root of embryos cultured in Duchefa neutralized AC was significantly different from those in Sigma neutralized AC only after one month from initial culture. For the succeeding periods (2‑4 months), no significant difiference was observed among the treatments in terms of increment in plant height, number of scale and true leaves and length ofprimary root. Statistical analysis revealed thatpercentage ofseedlings with primary, secondary and tertiary roots did not differ significantly among the treatments 1‑4 months from initial culture. Results suggest that any brand (even alternately) can be used satisfactorily in the in vitro culture of coconut embryos.

scholarly journals PHYSIOLOGICAL ASPECTS OF IN VITRO-GROWN COCONUT (Cocos nucifera L.) PLANTS DURING ACCLIMATIZATION

CORD ◽  
1999 ◽  
Vol 15 (02) ◽  
pp. 34
Author(s):  
C S Ranasinghe ◽  
L K Weerakoon ◽  
Y M H Liyanage ◽  
D T Mathes
Keyword(s):  
Light Intensity ◽  
Chlorophyll Content ◽  
Environmental Conditions ◽  
Cocos Nucifera ◽  
Low Light ◽  
Leaf Chlorophyll Content ◽  
Leaf Chlorophyll ◽  
Low Light Intensity ◽  
Cocos Nucifera L

The physiological status of in vitro-grown coconut (Cocos nucifera L.) plants during acclimatization was studied using nursery-raised seedlings as the control.  The percentage of open stomata in leaves of in vitro-grown coconut plants was high at the initial stage of acclimatization but decreased during the course of acclimatization indicating an improvement in stomatal regulation.  A progressive increase in the stomatal density, epicuticular wax deposition and leaf thickness in in vitro-grown plants was observed during acclimatization. As a result of the low light intensity, the epidermal cells of in vitro-grown plants were narrower and longer when compared to the control.  With the exposure of plants to increased light intensity, the cells became wider and shorter as observed in the control.   The leaf chlorophyll content was high in in vitro-grown plants under low light intensity.  With increasing light intensity, a reduction in leaf chlorophyll content in vitro-grown plants was observed and at the later stages of acclimatization, it was comparable to that of the control. Variations in the rates of photosynthesis and transpiration in vitro-grown plants were observed in response to the changing environmental conditions.  However, at the end of acclimatization, where the plants were ready to be transferred to the field, the physiological statuses of in vitro-grown coconut plants were comparable to that of nursery raised seedlings. The present study revealed that the embryo-cultured coconut plants could adjust well to the changing environmental conditions during acclimatization.

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