The 65-kDa cytosolic protein associated with warm temperature acclimation in goldfish, Carassius auratus

The effects of sex steroids on plasma lipids in female goldfish were studied in sexually regressed and maturing fish at 12 °C, and in recent postovulatory-regressed fish at 21 °C. Intraperitoneal injection of oestrone (E1), but not oestradiol (E2), raised plasma triglyceride (TG) concentrations in regressed fish, but oestrogen had no effect on plasma TG in either maturing fish at 12 °C or in recent postovulatory–regressed fish at 21 °C. Progesterone injection caused high levels of plasma TG in maturing fish at 12 °C. Fish injected with E1or E2 had higher plasma total cholesterol (TC) levels posttreatment compared with control fish in both experiments at 12 °C, but E1 and E2 were without effect on plasma TC at 21 °C. Both E1 and E2 raised plasma lipid phosphorus levels in all three experiments. Testosterone generally had little effect on plasma lipids. These results support the hypothesis that oestrogen is involved in lipid mobilization in teleosts, and it appears that this effect is sensitive to warm temperature. There was no support for a mammalian-like, progesterone-stimulated system for clearance of plasma TG in the female goldfish.

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Toxicity of Hydrogen Sulfide to Goldfish (Carassius auratus) as Influenced by Temperature, Oxygen, and Bioassay Techniques

Journal of the Fisheries Research Board of Canada ◽

10.1139/f72-199 ◽

1972 ◽

Vol 29 (9) ◽

pp. 1309-1317 ◽

Cited By ~ 20

Author(s):

Ira R. Adelman ◽

Lloyd L. Smith Jr.

Keyword(s):

Hydrogen Sulfide ◽

Carassius Auratus ◽

Temperature Acclimation ◽

Effect Of Temperature ◽

Goldfish Carassius Auratus ◽

The Mean ◽

Oxygen Concentrations

Bioassays were conducted to test the effect of temperature and oxygen on H2S toxicity to goldfish (Carassius auratus L.) and to investigate some factors that influence bioassay results. Relation of H2S toxicity to temperature is negatively logarithmic over the range of 6.5–25 C. The mean 96-hr TL50 at 6 C was 530 μg/liter and at 25 C was 4 μg/liter. At temperatures of 14, 20, and 26 C, most acute mortality from H2S ended by 11 days and the 11-day TL50's at these temperatures were significantly different. In bioassays with and without prior oxygen acclimation, decreasing oxygen concentrations increased H2S toxicity. In the former, mean TL50's were 62 and 48 μg/liter H2S at oxygen concentrations of 6 and 1.5 mg/liter, respectively, and in the latter, 71 and 53 μg/liter H2S at the same oxygen concentrations. Variability in bioassay results was not affected by test temperatures of 14, 20, and 26 C, and in most cases 1 week of temperature acclimation was adequate. Stocks of fish responded differently after 11 days of bioassay, although differences were not detected after 4 days of bioassay.

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SEM of Hepatocytes and Biliary Passages in Goldfish Liver

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080213 ◽

1977 ◽

Vol 35 ◽

pp. 556-557

Author(s):

Waykin Nopanitaya ◽

Joe W. Grisham ◽

Johnny L. Carson

Keyword(s):

Carassius Auratus ◽

Cell Layer ◽

Three Dimensional ◽

Cell Types ◽

Biliary System ◽

Fish Food ◽

Commercial Fish ◽

Goldfish Carassius Auratus ◽

Commercial Fish Food

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.

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