Variable versus constant temperature acclimation regimes: Effects on hemoglobin isomorph profile in goldfish,Carassius auratus

1994 ◽  
Vol 13 (6) ◽  
pp. 445-450 ◽  
Author(s):  
A. H. Houston ◽  
Julie H. Gingras-Bedard
Keyword(s):  
Constant Temperature ◽  
Carassius Auratus ◽  
Temperature Acclimation ◽  
Goldfish Carassius Auratus ◽  
Variable Versus

Toxicity of Hydrogen Sulfide to Goldfish (Carassius auratus) as Influenced by Temperature, Oxygen, and Bioassay Techniques

1972 ◽  
Vol 29 (9) ◽  
pp. 1309-1317 ◽  
Author(s):  
Ira R. Adelman ◽  
Lloyd L. Smith Jr.
Keyword(s):  
Hydrogen Sulfide ◽  
Carassius Auratus ◽  
Temperature Acclimation ◽  
Effect Of Temperature ◽  
Goldfish Carassius Auratus ◽  
The Mean ◽  
Oxygen Concentrations

Bioassays were conducted to test the effect of temperature and oxygen on H2S toxicity to goldfish (Carassius auratus L.) and to investigate some factors that influence bioassay results. Relation of H2S toxicity to temperature is negatively logarithmic over the range of 6.5–25 C. The mean 96-hr TL50 at 6 C was 530 μg/liter and at 25 C was 4 μg/liter. At temperatures of 14, 20, and 26 C, most acute mortality from H2S ended by 11 days and the 11-day TL50's at these temperatures were significantly different. In bioassays with and without prior oxygen acclimation, decreasing oxygen concentrations increased H2S toxicity. In the former, mean TL50's were 62 and 48 μg/liter H2S at oxygen concentrations of 6 and 1.5 mg/liter, respectively, and in the latter, 71 and 53 μg/liter H2S at the same oxygen concentrations. Variability in bioassay results was not affected by test temperatures of 14, 20, and 26 C, and in most cases 1 week of temperature acclimation was adequate. Stocks of fish responded differently after 11 days of bioassay, although differences were not detected after 4 days of bioassay.

Hemoglobin Levels and Red Cell Ionic Composition in Goldfish (Carassius auratus) Exposed to Constant and Diurnally Cycling Temperatures

1981 ◽  
Vol 38 (10) ◽  
pp. 1182-1188 ◽  
Author(s):  
Ted F. Koss ◽  
Arthur H. Houston
Keyword(s):  
Constant Temperature ◽  
Carrying Capacity ◽  
Carassius Auratus ◽  
Ionic Composition ◽  
Oxygen Affinity ◽  
Blood Chemistry ◽  
Temperature Cycle ◽  
Red Cell ◽  
Temperature Conditions ◽  
Goldfish Carassius Auratus

Hematological response in goldfish (Carassius auratus) held at constant temperature (20, 25, 30 °C) was compared with that of animals exposed to a diurnal temperature cycle (25 ± 5 °C), with particular reference to overall oxygen carrying capacity and cellular electrolytes influencing haemoglobin–oxygen affinity. All groups were sampled at 03:00, 09:00, 15:00, and 21:00. Hemoglobin and hematocrit were determined, as were plasma and packed red cell levels of water, sodium, potassium, magnesium, calcium, and chloride. The latter were used to estimate mean cellular ion concentrations. Temperature treatment influenced response. Under constant temperature conditions hemoglobin levels tended to increase at higher temperatures. This was associated with compositional changes which would be expected to reduce affinity and facilitate oxygen release. By contrast, under cycling temperature conditions hemoglobin levels were elevated whereas erythrocytic electrolyte concentrations tended to be stable. Observations are discussed in terms of their bearing on the interpretation of studies on the acclimatory process.Key words: goldfish, Carassius auratus; blood chemistry, temperature, hemoglobin, O2-carrying capacity

SEM of Hepatocytes and Biliary Passages in Goldfish Liver

1977 ◽  
Vol 35 ◽  
pp. 556-557
Author(s):  
Waykin Nopanitaya ◽  
Joe W. Grisham ◽  
Johnny L. Carson
Keyword(s):  
Carassius Auratus ◽  
Cell Layer ◽  
Three Dimensional ◽  
Cell Types ◽  
Biliary System ◽  
Fish Food ◽  
Commercial Fish ◽  
Goldfish Carassius Auratus ◽  
Commercial Fish Food

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.

Induction of Gynogenesis and Androgenesis in Goldfish Carassius auratus (var. oranda)

2001 ◽  
Vol 36 (3-4) ◽  
pp. 195-198 ◽  
Author(s):  
I Paschos ◽  
L Natsis ◽  
C Nathanailides ◽  
I Kagalou ◽  
E Kolettas
Keyword(s):  
Carassius Auratus ◽  
Goldfish Carassius Auratus
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