scholarly journals Identification of Brassica oleracea monosomic alien chromosome addition lines with molecular markers reveals extensive gene duplication

1990 ◽  
Vol 223 (2) ◽  
pp. 198-204 ◽  
Author(s):  
J. Mitchell McGrath ◽  
Carlos F. Quiros ◽  
John J. Harada ◽  
Benoit S. Landry
Keyword(s):  
Molecular Markers ◽  
Gene Duplication ◽  
Brassica Oleracea ◽  
Alien Chromosome ◽  
Addition Lines ◽  
Chromosome Addition ◽  
Chromosome Addition Lines ◽  
Alien Chromosome Addition

scholarly journals Identification of wheat alien chromosome addition lines for breeding wheat with high phosphorus efficiency

2010 ◽  
Vol 60 (4) ◽  
pp. 371-379 ◽  
Author(s):  
Shiwen Wang ◽  
Lina Yin ◽  
Hiroyuki Tanaka ◽  
Kiyoshi Tanaka ◽  
Hisashi Tsujimoto
Keyword(s):  
Phosphorus Efficiency ◽  
Alien Chromosome ◽  
Addition Lines ◽  
Chromosome Addition ◽  
High Phosphorus ◽  
Chromosome Addition Lines ◽  
Alien Chromosome Addition

Generation of alien chromosome addition lines from synthetic Brassica napus: morphology, cytology, fertility, and chromosome transmission

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 374-383 ◽  
Author(s):  
J. Mitchell McGrath ◽  
Carlos F. Quiros
Keyword(s):  
Chromosome Complement ◽  
Alien Chromosome ◽  
Addition Lines ◽  
Backcross Generation ◽  
Seed Fertility ◽  
Chromosome Addition ◽  
Chromosome Transmission ◽  
Chromosome Addition Lines ◽  
Alien Chromosome Addition ◽  
Monosomic Addition

The chromosome complement of Brassica oleracea (2n = 18) was dissected by means of alien chromosome addition lines generated by successive backcrosses of either of two B. campestris accessions (2n = 20) to the resynthesized B. napus 'Hakuran' (2n = 38). Alien chromosome addition lines were characterized by chromosome counts, morphology, pollen and seed fertility, and transmission of chromosome-specific markers. Mean chromosome number in the first backcross generation was approximately 23.5 and was little influenced by the B. campestris accession. Fertility and isozyme marker transmission were also not affected by choice of B. campestris accession. Transmission of chromosome-specific markers to the BC2 was more variable than to the BC1, and appeared to be affected by the B. campestris recurrent accession. Twenty-five monosomic addition lines (2n = 21) were recovered in the second backcross generation, representing 7 of the 9 B. oleracea synteny groups. One monosomic alien chromosome decreased seed fertility but not pollen fertility. Only one monosomic addition could be reliably identified morphologically.Key words: chromosome markers, aneuploidy, restriction fragment length polymorphism, isoymes.

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