scholarly journals Identification of wheat alien chromosome addition lines for breeding wheat with high phosphorus efficiency

2010 ◽  
Vol 60 (4) ◽  
pp. 371-379 ◽  
Author(s):  
Shiwen Wang ◽  
Lina Yin ◽  
Hiroyuki Tanaka ◽  
Kiyoshi Tanaka ◽  
Hisashi Tsujimoto
Keyword(s):  
Phosphorus Efficiency ◽  
Alien Chromosome ◽  
Addition Lines ◽  
Chromosome Addition ◽  
High Phosphorus ◽  
Chromosome Addition Lines ◽  
Alien Chromosome Addition

Generation of alien chromosome addition lines from synthetic Brassica napus: morphology, cytology, fertility, and chromosome transmission

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 374-383 ◽  
Author(s):  
J. Mitchell McGrath ◽  
Carlos F. Quiros
Keyword(s):  
Chromosome Complement ◽  
Alien Chromosome ◽  
Addition Lines ◽  
Backcross Generation ◽  
Seed Fertility ◽  
Chromosome Addition ◽  
Chromosome Transmission ◽  
Chromosome Addition Lines ◽  
Alien Chromosome Addition ◽  
Monosomic Addition

The chromosome complement of Brassica oleracea (2n = 18) was dissected by means of alien chromosome addition lines generated by successive backcrosses of either of two B. campestris accessions (2n = 20) to the resynthesized B. napus 'Hakuran' (2n = 38). Alien chromosome addition lines were characterized by chromosome counts, morphology, pollen and seed fertility, and transmission of chromosome-specific markers. Mean chromosome number in the first backcross generation was approximately 23.5 and was little influenced by the B. campestris accession. Fertility and isozyme marker transmission were also not affected by choice of B. campestris accession. Transmission of chromosome-specific markers to the BC2 was more variable than to the BC1, and appeared to be affected by the B. campestris recurrent accession. Twenty-five monosomic addition lines (2n = 21) were recovered in the second backcross generation, representing 7 of the 9 B. oleracea synteny groups. One monosomic alien chromosome decreased seed fertility but not pollen fertility. Only one monosomic addition could be reliably identified morphologically.Key words: chromosome markers, aneuploidy, restriction fragment length polymorphism, isoymes.

scholarly journals Production of disomic wheat-barley chromosome addition lines using Hordeum bulbosum crosses

1981 ◽  
Vol 37 (2) ◽  
pp. 215-219 ◽  
Author(s):  
A. K. M. R. Islam ◽  
K. W. Shepherd
Keyword(s):  
Female Parent ◽  
Barley Chromosome ◽  
Hordeum Bulbosum ◽  
Alien Chromosome ◽  
Addition Lines ◽  
Transmission Frequency ◽  
Chromosome Addition ◽  
Chromosome Addition Lines ◽  
Disomic Addition Lines ◽  
Wheat Parent

SUMMARYThe possibility of using Hordeum bulbosum Crosses to facilitate production of disomic wheat–barley addition lines from monosomic additions was investigated. Aneuhaploids with 22 chromosomes were obtained in the expected gametic frequencies after crossing monosomic, disomio and monotelo-disomic addition lines, involving four different barley chromosomes, as the female parent with tetraploid H. bulbosum. Thus the added barley chromosomes were not eliminated when preferential elimination of the bulbosum chromosomes took place in the hybrid embryos. Disomic addition lines were obtained after treating the aneuhaploids with colchicine. This method could have wider application in the production of other wheat–alien chromosome disomic addition lines, especially where the transmission frequency of the alien chromosome through the pollen is very low, but its use will depend on the wheat parent being crossarle with H. bulbosum and the alien chromosome being retained during the elimination of bulbosum chromosomes.

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